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Endophytic actinomycetes were isolated from the gel of <jats:italic>A. ferox<\/jats:italic> Mill by surface sterilization technique using actinomycete isolation agar. The isolate with a promising antibacterial activity was identified using 16S rRNA sequence analysis. The minimum inhibitory concentration (MIC) of the extract was assessed by the micro-dilution method and its effect on the respiratory chain dehydrogenase (RCD) activity was ascertained by the iodonitrotetrazolium chloride (INT) assay. Fourier transform-infrared spectrophotometer (FTIR) and gas chromatography-mass spectrophotometry (GC-MS) were employed to identify functional groups and the chemical constituents, respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The actinobacterium was found to be <jats:italic>Streptomyces olivaceus<\/jats:italic> CP016795.1. Its extract displayed noteworthy antibacterial activity (MIC \u22641\u2009mg\/mL) against <jats:italic>Staphylococcus aureus<\/jats:italic> (ATCC 25925), <jats:italic>Bacillus cereus<\/jats:italic> (ATCC 10102), and <jats:italic>Escherichia coli<\/jats:italic> (ATCC 25922); and showed an inhibitory effect on the RCD activity. FTIR spectrum displayed hydroxyl, amine, and aromatic groups, and the GC\u2013MS revealed 5-Hydroxymethylfurfural as the main constituent (19.47%).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p><jats:italic>S. olivaceus<\/jats:italic> CP016795.1 can serve as a potential source of effective antibacterial compounds.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12906-022-03733-8","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T12:02:51Z","timestamp":1664798571000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity"],"prefix":"10.1186","volume":"22","author":[{"given":"Tsolanku Sidney","family":"Maliehe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melusi","family":"Mbambo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Londeka Sibusisiwe","family":"Ngidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jabulani Siyabonga Emmanuel","family":"Shandu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ofentse Jacob","family":"Pooe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Masoko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tlou Nelson","family":"Selepe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"issue":"11","key":"3733_CR1","doi-asserted-by":"publisher","first-page":"6432","DOI":"10.1016\/j.sjbs.2021.07.009","volume":"28","author":"N Mujaddidi","year":"2021","unstructured":"Mujaddidi N, Nisa S, Al Ayoubi S, Bibi Y, Khan S, Sabir M, et al. 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