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The resistance of biofilms to antibiotics has led to a search for alternative approaches in biofilm elimination. Antioxidants work synergistically with antibacterial agents against biofilms. Hence recent research has suggested plants as candidates in the development of new alternatives in biofilm treatments and as antioxidants due to the presence of phytocompounds which are responsible for their bioactivities. <jats:italic>Hoslundi<\/jats:italic>a <jats:italic>opposita Vahl<\/jats:italic> is one of the plants used by traditional healers to treat wounds and other infections, this makes it a potential candidate for drug discovery hence, in this study, we investigate the antibiofilm and antioxidant activity of methanolic extract of <jats:italic>hoslundia opposita V<\/jats:italic>ahl from Uganda. We also identify phytochemicals responsible for its bioactivity.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Method<\/jats:title>\n                <jats:p>the plant was extracted by maceration using methanol, and the extract was investigated for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. The antibiofilm activity using microtiter plate assay (MTP) assay where the Minimum biofilm inhibitory concentration required to inhibit 50% or 90% of the biofilm (MBIC<jats:sub>50<\/jats:sub> and MBIC<jats:sub>90<\/jats:sub>) and Minimum biofilm eradication concentration required to remove 50% or 90% of the biofilm (MBEC<jats:sub>50<\/jats:sub> and MBEC<jats:sub>90<\/jats:sub>) were measured. It was further analysed for its phytochemical composition using quantitative screening, as well as Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography mass-spectrometry (LC-MS).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>H. Opposita Vahl<\/jats:italic> extract showed good antioxidant activity with of 249.6\u00a0mg\/mL. It inhibited the growth of <jats:italic>P. aeruginosa<\/jats:italic> and <jats:italic>S. aureus biofilms with<\/jats:italic> MBIC<jats:sub>50<\/jats:sub> of 28.37\u00a0mg\/mL and 10\u00a0mg\/mL, respectively. It showed the ability to eradicate <jats:italic>P. aeruginosa<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> biofilms with MBEC<jats:sub>50<\/jats:sub> of 23.85 and 39.01\u00a0mg\/mL respectively. Phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, and phenols. GC-MS analysis revealed 122 compounds in the extract of which, 23 have evidence of antioxidant or antibiofilm activity in literature. The most abundant compounds were; 1,4- Citric acid, Tetracontane-1,40-diol (43.43.3%, 1, Olean-12-en-28-oic acid, 3-hydroxy-, methyl ester, (3.beta) (15.36%) 9-Octadecenamide (12.50%), Squalene (11.85%) Palmitic Acid 4TMS (11.28%), and alpha Amyrin (11.27%). The LC-MS identified 115 and 57 compounds in multiple reaction mode (MRM) and scan modes respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p><jats:italic>H. opposita Vahl<\/jats:italic> showed antibiofilm and antioxidant activity due to bioactive compounds identified, hence the study justifies its use for wound healing. It can be utilised in further development of new drugs as antibiofilm and antioxidants.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12906-024-04540-z","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T09:01:45Z","timestamp":1718614905000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing"],"prefix":"10.1186","volume":"24","author":[{"given":"Catherine","family":"Namuga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruna","family":"Muwonge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerebba","family":"Nasifu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Sekandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahalu","family":"Sekulima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Baptist","family":"Kirabira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"issue":"7","key":"4540_CR1","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1089\/wound.2014.0557","volume":"4","author":"SL Percival","year":"2015","unstructured":"Percival SL, McCarty SM, Lipsky B. 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