{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:09:09Z","timestamp":1791223749610,"version":"4.1.0"},"reference-count":89,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Military Nursing Innovation and Incubation Project","award":["2021HL098"],"award-info":[{"award-number":["2021HL098"]}]},{"name":"Nursing Innovation and Incubation Program of PLA General Hospital Sixth Medical Center","award":["HLCX202105"],"award-info":[{"award-number":["HLCX202105"]}]},{"name":"Youth Incubation Program of Medical Science and Technology of PLA","award":["21QNPY114"],"award-info":[{"award-number":["21QNPY114"]}]},{"name":"Beijing municipal education commission general project","award":["KM202010011010"],"award-info":[{"award-number":["KM202010011010"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Complement Med Ther"],"DOI":"10.1186\/s12906-025-05007-5","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:20:39Z","timestamp":1753183239000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Investigation of differential Multi-Mode antibacterial mechanisms of essential oils of Satureja montana L. and Leptospermum scoparium J.R.Forst. &amp; G.Forst. Against Porphyromonas gingivalis"],"prefix":"10.1186","volume":"25","author":[{"given":"Yue","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuli","family":"Hui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rifat Nowshin","family":"Raka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junsong","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongwei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"5007_CR1","doi-asserted-by":"crossref","unstructured":"Kinane DF. Causation and pathogenesis of periodontal disease. Periodontol 2000. 2001;25(1):8\u201320.","DOI":"10.1034\/j.1600-0757.2001.22250102.x"},{"key":"5007_CR2","doi-asserted-by":"publisher","first-page":"53","DOI":"10.3389\/fmicb.2016.00053","volume":"7","author":"KY How","year":"2016","unstructured":"How KY, Song KP, Chan KG. Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016;7:53.","journal-title":"Front Microbiol"},{"key":"5007_CR3","doi-asserted-by":"publisher","first-page":"104483","DOI":"10.1016\/j.archoralbio.2019.104483","volume":"107","author":"L Zhang","year":"2019","unstructured":"Zhang L, Gao L, Xu C, Li X, Wang P, Zhang C, Zhao C. Porphyromonas gingivalis lipopolysaccharide promotes T- helper 17 cell differentiation from human CD4+ Na\u00efve T cells via toll-like receptor-2 in vitro. Arch Oral Biol. 2019;107:104483.","journal-title":"Arch Oral Biol"},{"key":"5007_CR4","doi-asserted-by":"publisher","first-page":"262","DOI":"10.3389\/fcimb.2019.00262","volume":"9","author":"L Jia","year":"2019","unstructured":"Jia L, Han N, Du J, Guo L, Luo Z, Liu Y. Pathogenesis of important virulence factors of Porphyromonas gingivalis via toll-like receptors. Front Cell Infect Microbiol. 2019;9:262.","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"5007_CR5","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1111\/prd.12337","volume":"84","author":"C Janakiram","year":"2020","unstructured":"Janakiram C, Dye BA. A public health approach for prevention of periodontal disease. Periodontol 2000. 2020;84(1):202\u201314.","journal-title":"Periodontol 2000"},{"issue":"6","key":"5007_CR6","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1902\/jop.2002.73.6.675","volume":"73","author":"R Buchmann","year":"2002","unstructured":"Buchmann R, Nunn ME, Van Dyke TE, Lange DE. Aggressive periodontitis: 5-year follow-up of treatment. J Periodontol. 2002;73(6):675\u201383.","journal-title":"J Periodontol"},{"issue":"12","key":"5007_CR7","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1111\/jcpe.12004","volume":"39","author":"M Feres","year":"2012","unstructured":"Feres M, Soares GM, Mendes JA, Silva MP, Faveri M, Teles R, Socransky SS, Figueiredo LC. Metronidazole alone or with amoxicillin as adjuncts to non-surgical treatment of chronic periodontitis: a 1-year double-blinded, placebo-controlled, randomized clinical trial. J Clin Periodontol. 2012;39(12):1149\u201358.","journal-title":"J Clin Periodontol"},{"issue":"3","key":"5007_CR8","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1902\/jop.2014.140478","volume":"86","author":"A Mombelli","year":"2015","unstructured":"Mombelli A, Almaghlouth A, Cionca N, Courvoisier DS, Giannopoulou C. Differential benefits of amoxicillin\u2013metronidazole in different phases of periodontal therapy in a randomized controlled crossover clinical trial. J Periodontol. 2015;86(3):367\u201375.","journal-title":"J Periodontol"},{"issue":"Suppl 22","key":"5007_CR9","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1111\/jcpe.13264","volume":"47","author":"W Teughels","year":"2020","unstructured":"Teughels W, Feres M, Oud V, Mart\u00edn C, Matesanz P, Herrera D. Adjunctive effect of systemic antimicrobials in periodontitis therapy: A systematic review and meta-analysis. J Clin Periodontol. 2020;47(Suppl 22):257\u201381.","journal-title":"J Clin Periodontol"},{"key":"5007_CR10","volume-title":"Periodontology - New insights","author":"I-G Sufaru","year":"2022","unstructured":"Sufaru I-G, Martu M-A, Luchian I, Teslaru S, Stoleriu S, Stratul S-I, Rusu D, Tatarciuc MS, Solomon SM. Advances in locally delivered antimicrobials for periodontitis treatment. In: Sridharan G, editor. Periodontology - New insights. Rijeka: IntechOpen; 2022."},{"issue":"5","key":"5007_CR11","doi-asserted-by":"publisher","first-page":"430","DOI":"10.3390\/antibiotics13050430","volume":"13","author":"I Ilyes","year":"2024","unstructured":"Ilyes I, Boariu M, Rusu D, Iorio-Siciliano V, Vela O, Boia S, Radulescu V, \u0218urlin P, Jentsch H, Lodin A, et al. Comparative study of systemic vs. local antibiotics with subgingival instrumentation in stage III\u2013IV periodontitis: A retrospective analysis. Antibiotics. 2024;13(5):430.","journal-title":"Antibiotics"},{"key":"5007_CR12","doi-asserted-by":"crossref","unstructured":"Rams TE, Sautter JD, van Winkelhoff AJ. Emergence of antibiotic-resistant Porphyromonas gingivalis in united States periodontitis patients. Antibiot (Basel). 2023; 12(11).","DOI":"10.3390\/antibiotics12111584"},{"key":"5007_CR13","doi-asserted-by":"publisher","first-page":"143956","DOI":"10.1016\/j.chemosphere.2024.143956","volume":"370","author":"U Salma","year":"2025","unstructured":"Salma U, Nishimura Y, Tokumura M, Hossain A, Watanabe K, Noro K, Raknuzzaman M, Amagai T, Makino M. Occurrence, seasonal variation, and environmental risk of multiclass antibiotics in the urban surface water of the Buriganga river, Bangladesh. Chemosphere. 2025;370:143956.","journal-title":"Chemosphere"},{"key":"5007_CR14","doi-asserted-by":"crossref","unstructured":"Abdulkareem A, Abdulbaqi H, Gul S, Milward M, Chasib N, Alhashimi R. Classic vs. novel antibacterial approaches for eradicating dental biofilm as adjunct to periodontal debridement: an evidence-based overview. Antibiot (Basel). 2021; 11(1).","DOI":"10.3390\/antibiotics11010009"},{"key":"5007_CR15","doi-asserted-by":"crossref","unstructured":"Abdallah EM, Alhatlani BY, de Paula Menezes R, Martins CHG. Back to nature: medicinal plants as promising sources for antibacterial drugs in the post-antibiotic era. Plants (Basel) 2023, 12(17).","DOI":"10.3390\/plants12173077"},{"issue":"3","key":"5007_CR16","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","volume":"20","author":"AG Atanasov","year":"2021","unstructured":"Atanasov AG, Zotchev SB, Dirsch VM, Orhan IE, Banach M, Rollinger JM, Barreca D, Weckwerth W, Bauer R, Bayer EA, et al. Natural products in drug discovery: advances and opportunities. Nat Rev Drug Discovery. 2021;20(3):200\u201316.","journal-title":"Nat Rev Drug Discovery"},{"issue":"3","key":"5007_CR17","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1007\/s10787-024-01631-8","volume":"33","author":"MA Anwar","year":"2025","unstructured":"Anwar MA, Sayed GA, Hal DM, Hafeez M, Shatat AS, Salman A, Eisa NM, Ramadan A, El-Shiekh RA, Hatem S, et al. Herbal remedies for oral and dental health: a comprehensive review of their multifaceted mechanisms including antimicrobial, anti-inflammatory, and antioxidant pathways. Inflammopharmacology. 2025;33(3):1085\u2013160.","journal-title":"Inflammopharmacology"},{"issue":"18","key":"5007_CR18","doi-asserted-by":"publisher","first-page":"e37641","DOI":"10.1016\/j.heliyon.2024.e37641","volume":"10","author":"MS Refaey","year":"2024","unstructured":"Refaey MS, Abosalem EF, Yasser El-Basyouni R, Elsheriri SE, Elbehary SH, Fayed MAA. Exploring the therapeutic potential of medicinal plants and their active principles in dental care: A comprehensive review. Heliyon. 2024;10(18):e37641.","journal-title":"Heliyon"},{"key":"5007_CR19","doi-asserted-by":"crossref","unstructured":"Kumar M, Prakash S, Radha, Kumari N, Pundir A, Punia S, Saurabh V, Choudhary P, Changan S, Dhumal S et al. Beneficial role of antioxidant secondary metabolites from medicinal plants in maintaining oral health. Antioxid (Basel). 2021; 10(7).","DOI":"10.3390\/antiox10071061"},{"key":"5007_CR20","doi-asserted-by":"crossref","unstructured":"Maccelli A, Vitanza L, Imbriano A, Fraschetti C, Filippi A, Goldoni P, Maurizi L, Ammendolia MG, Crestoni ME, Fornarini S et al. Satureja montana L. essential oils: chemical profiles\/phytochemical screening, antimicrobial activity and O\/W nanoemulsion formulations. Pharmaceutics. 2019; 12(1).","DOI":"10.3390\/pharmaceutics12010007"},{"key":"5007_CR21","first-page":"119","volume":"8","author":"H Said-Al Ahl","year":"2016","unstructured":"Said-Al Ahl H, Wahby MS. Effect of nitrogen and phosphorus application on herb and essential oil composition of Satureja montana L. \u2018carvacrol\u2019 chemotype. J Chem Pharm Res. 2016;8:119\u201328.","journal-title":"J Chem Pharm Res"},{"key":"5007_CR22","doi-asserted-by":"crossref","unstructured":"Said-Al Ahl HAH, Ka\u010d\u00e1niova M, Mahmoud AA, Hikal WM, \u010cmikov\u00e1 N, Szczepanek M, B\u0142aszczyk K, Al-Balawi SM, Bianchi A, Smaoui S et al. Phytochemical characterization and biological activities of essential oil from Satureja montana L., a medicinal plant grown under the influence of fertilization and planting dates. Biology (Basel). 2024; 13(5).","DOI":"10.3390\/biology13050328"},{"issue":"3","key":"5007_CR23","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1016\/j.ijfoodmicro.2010.11.022","volume":"144","author":"TLC de Oliveira","year":"2011","unstructured":"de Oliveira TLC, de Ara\u00fajo Soares R, Ramos EM, das Gra\u00e7as Cardoso M, Alves E, Piccoli RH. Antimicrobial activity of Satureja montana L. essential oil against Clostridium perfringens type A inoculated in mortadella-type sausages formulated with different levels of sodium nitrite. Int J Food Microbiol. 2011;144(3):546\u201355.","journal-title":"Int J Food Microbiol"},{"key":"5007_CR24","doi-asserted-by":"crossref","unstructured":"Chizzola R. Volatile oil composition of four populations of Satureja Montana L. from Southern France. Acta Hort. 2003;598.","DOI":"10.17660\/ActaHortic.2003.598.20"},{"key":"5007_CR25","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.foodchem.2014.10.042","volume":"173","author":"SM Nabavi","year":"2015","unstructured":"Nabavi SM, Marchese A, Izadi M, Curti V, Daglia M, Nabavi SF. Plants belonging to the genus Thymus as antibacterial agents: from farm to pharmacy. Food Chem. 2015;173:339\u201347.","journal-title":"Food Chem"},{"key":"5007_CR26","first-page":"879","volume":"80","author":"S Bagi\u0144ska","year":"2024","unstructured":"Bagi\u0144ska S, Golonko A, \u015awis\u0142ocka R, Lewandowski W. Monoterpenes as medicinal agents: exploring the pharmaceutical potential of p-cymene, p-cymenene, and \u03b3-terpinene. Acta Pol Pharm - Drug Res. 2024;80:879\u201392.","journal-title":"Acta Pol Pharm - Drug Res"},{"key":"5007_CR27","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/B978-0-323-90788-0.00002-0","volume-title":"Biochemistry, nutrition, and therapeutics of black Cumin seed","author":"H Ak","year":"2023","unstructured":"Ak H, G\u00dcL\u015eEn \u0130, Canbek \u0130. Chapter Seventeen - Neuroprotective effects of black Cumin seed and seed oil. In: Mariod AA, editor. Biochemistry, nutrition, and therapeutics of black Cumin seed. San Diego: Academic; 2023. pp. 201\u201330."},{"key":"5007_CR28","doi-asserted-by":"crossref","unstructured":"Cical\u0103u GIP, Babes PA, Calniceanu H, Popa A, Ciavoi G, Iova GM, Ganea M, Scrobot\u0103 I. Anti-inflammatory and antioxidant properties of carvacrol and magnolol, in periodontal disease and diabetes mellitus. Molecules. 2021; 26(22).","DOI":"10.3390\/molecules26226899"},{"key":"5007_CR29","doi-asserted-by":"publisher","first-page":"106004","DOI":"10.1016\/j.archoralbio.2024.106004","volume":"164","author":"FL Kendlbacher","year":"2024","unstructured":"Kendlbacher FL, Bloch S, Hager-Mair FF, Sch\u00e4ffer C, Andrukhov O. Red-complex bacteria exhibit distinctly different interactions with human periodontal ligament stromal cells compared to Fusobacterium nucleatum. Arch Oral Biol. 2024;164:106004.","journal-title":"Arch Oral Biol"},{"key":"5007_CR30","doi-asserted-by":"crossref","unstructured":"Ben Lagha A, Vaillancourt K, Maquera Huacho P, Grenier D. Effects of Labrador tea, peppermint, and winter savory essential oils on Fusobacterium nucleatum. Antibiot (Basel). 2020; 9(11).","DOI":"10.3390\/antibiotics9110794"},{"issue":"1","key":"5007_CR31","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1038\/s41522-019-0084-7","volume":"5","author":"A El-Awady","year":"2019","unstructured":"El-Awady A, de Sousa Rabelo M, Meghil MM, Rajendran M, Elashiry M, Stadler AF, Foz AM, Susin C, Romito GA, Arce RM, et al. Polymicrobial synergy within oral biofilm promotes invasion of dendritic cells and survival of consortia members. Npj Biofilms Microbiomes. 2019;5(1):11.","journal-title":"Npj Biofilms Microbiomes"},{"key":"5007_CR32","doi-asserted-by":"crossref","unstructured":"Groeger S, Zhou Y, Ruf S, Meyle J. Pathogenic mechanisms of Fusobacterium nucleatum on oral epithelial cells. Frontiers in Oral Health. 2022; 3:2022.","DOI":"10.3389\/froh.2022.831607"},{"issue":"3","key":"5007_CR33","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1111\/j.1574-695X.2008.00481.x","volume":"54","author":"A Saito","year":"2008","unstructured":"Saito A, Inagaki S, Kimizuka R, Okuda K, Hosaka Y, Nakagawa T, Ishihara K. Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis. FEMS Immunol Med Microbiol. 2008;54(3):349\u201355.","journal-title":"FEMS Immunol Med Microbiol"},{"issue":"6","key":"5007_CR34","doi-asserted-by":"publisher","first-page":"619","DOI":"10.3390\/biom14060619","volume":"14","author":"M Antoniadou","year":"2024","unstructured":"Antoniadou M, Rozos G, Vaou N, Zaralis K, Ersanli C, Alexopoulos A, Dadamogia A, Varzakas T, Tzora A, Voidarou C. Comprehensive bio-screening of phytochemistry and biological capacity of oregano (Origanum vulgare) and salvia triloba extracts against oral cariogenic and food-origin pathogenic bacteria. Biomolecules. 2024;14(6):619.","journal-title":"Biomolecules"},{"issue":"4","key":"5007_CR35","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1177\/2156587217700772","volume":"22","author":"M Fani","year":"2017","unstructured":"Fani M, Kohanteb J. In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. J Evidence-Based Complement Altern Med. 2017;22(4):660\u20136.","journal-title":"J Evidence-Based Complement Altern Med"},{"issue":"6","key":"5007_CR36","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1080\/08927014.2018.1485892","volume":"34","author":"PM Maquera-Huacho","year":"2018","unstructured":"Maquera-Huacho PM, Coradi TC, Ferreira CM, Serignoli FR, Ferreira BEA, \u00c9lcio M, Spolidorio DMP. Vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces. Biofouling. 2018;34(6):699\u2013709.","journal-title":"Biofouling"},{"key":"5007_CR37","doi-asserted-by":"crossref","unstructured":"Mathew C, Tesfaye W, Rasmussen P, Peterson GM, Bartholomaeus A, Sharma M, Thomas J. M\u0101nuka oil - a review of antimicrobial and other medicinal properties. Pharmaceuticals (Basel). 2020; 13(11).","DOI":"10.3390\/ph13110343"},{"issue":"6","key":"5007_CR38","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1080\/10412905.1999.9712001","volume":"11","author":"F Christoph","year":"1999","unstructured":"Christoph F, H. KK, and, Stahl-Biskup E. The composition of commercial Manuka oils from new Zealand. J Essent Oil Res. 1999;11(6):705\u201310.","journal-title":"J Essent Oil Res"},{"issue":"1","key":"5007_CR39","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1080\/0028825X.1998.9512551","volume":"36","author":"NG Porter","year":"1998","unstructured":"Porter NG, SP E, NM A, HA J, W. VKJ, and, Dean CM. Variability in essential oil chemistry and plant morphology within a Leptospermum scoparium population. N Z J Bot. 1998;36(1):125\u201333.","journal-title":"N Z J Bot"},{"issue":"9","key":"5007_CR40","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1016\/j.phytochem.2004.03.019","volume":"65","author":"MH Douglas","year":"2004","unstructured":"Douglas MH, van Klink JW, Smallfield BM, Perry NB, Anderson RE, Johnstone P, Weavers RT. Essential oils from new Zealand manuka: triketone and other chemotypes of Leptospermum scoparium. Phytochemistry. 2004;65(9):1255\u201364.","journal-title":"Phytochemistry"},{"issue":"8","key":"5007_CR41","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1055\/s-2001-18350","volume":"67","author":"F Christoph","year":"2001","unstructured":"Christoph F, Kaulfers PM, Stahl-Biskup E. Vitro evaluation of the antibacterial activity of beta-triketones admixed to Melaleuca oils. Planta Med. 2001;67(8):768\u201371.","journal-title":"Planta Med"},{"key":"5007_CR42","doi-asserted-by":"publisher","first-page":"156321","DOI":"10.1016\/j.phymed.2024.156321","volume":"136","author":"NA Nammunige","year":"2025","unstructured":"Nammunige NA, Agnew-Francis KA, Fernando DD, Taylor S, Lu H, Chow S, Hartel G, Okano S, Williams CM. Fischer K: \u03b2-Triketones from Leptospermum scoparium (m\u0101nuka) oil show potential as scabicides. Phytomedicine. 2025;136:156321.","journal-title":"Phytomedicine"},{"issue":"4","key":"5007_CR43","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1111\/j.1399-302X.2005.00216.x","volume":"20","author":"SK Filoche","year":"2005","unstructured":"Filoche SK, Soma K, Sissons CH. Antimicrobial effects of essential oils in combination with chlorhexidine digluconate. Oral Microbiol Immunol. 2005;20(4):221\u20135.","journal-title":"Oral Microbiol Immunol"},{"issue":"2","key":"5007_CR44","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.ejon.2009.01.002","volume":"13","author":"W Maddocks-Jennings","year":"2009","unstructured":"Maddocks-Jennings W, Wilkinson JM, Cavanagh HM, Shillington D. Evaluating the effects of the essential oils Leptospermum scoparium (manuka) and Kunzea ericoides (kanuka) on radiotherapy induced mucositis: a randomized, placebo controlled feasibility study. Eur J Oncol Nurs. 2009;13(2):87\u201393.","journal-title":"Eur J Oncol Nurs"},{"issue":"3","key":"5007_CR45","doi-asserted-by":"publisher","first-page":"212","DOI":"10.4103\/0970-4388.135827","volume":"32","author":"S Rupesh","year":"2014","unstructured":"Rupesh S, Winnier JJ, Nayak UA, Rao AP, Reddy NV, Peter J. Evaluation of the effects of Manuka honey on salivary levels of mutans Streptococci in children: a pilot study. J Indian Soc Pedod Prev Dent. 2014;32(3):212\u20139.","journal-title":"J Indian Soc Pedod Prev Dent"},{"issue":"1","key":"5007_CR46","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1046\/j.0902-0055.2003.00111.x","volume":"19","author":"K Takarada","year":"2004","unstructured":"Takarada K, Kimizuka R, Takahashi N, Honma K, Okuda K, Kato T. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol. 2004;19(1):61\u20134.","journal-title":"Oral Microbiol Immunol"},{"issue":"8","key":"5007_CR47","doi-asserted-by":"publisher","first-page":"3261","DOI":"10.1128\/IAI.01727-14","volume":"82","author":"AW Aruni","year":"2014","unstructured":"Aruni AW, Zhang K, Dou Y, Fletcher H. Proteome analysis of coinfection of epithelial cells with Filifactor alocis and Porphyromonas gingivalis shows modulation of pathogen and host regulatory pathways. Infect Immun. 2014;82(8):3261\u201374.","journal-title":"Infect Immun"},{"issue":"1","key":"5007_CR48","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1186\/s12906-023-03890-4","volume":"23","author":"Y Yuan","year":"2023","unstructured":"Yuan Y, Sun J, Song Y, Raka RN, Xiang J, Wu H, Xiao J, Jin J, Hui X. Antibacterial activity of oregano essential oils against Streptococcus mutans in vitro and analysis of active components. BMC Complement Med Ther. 2023;23(1):61.","journal-title":"BMC Complement Med Ther"},{"issue":"7","key":"5007_CR49","doi-asserted-by":"publisher","first-page":"e16585","DOI":"10.1111\/jfpp.16585","volume":"46","author":"Y Yuan","year":"2022","unstructured":"Yuan Y, Geng X, Wu H, Kumar R, Wang J, Xiao J, Tian H. Chemical composition, antimicrobial activities, and microencapsulation by complex coacervation of tea tree essential oils. J Food Process Preserv. 2022;46(7):e16585.","journal-title":"J Food Process Preserv"},{"issue":"3","key":"5007_CR50","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1111\/j.1601-5037.2009.00389.x","volume":"7","author":"I Rasooli","year":"2009","unstructured":"Rasooli I, Shayegh S, Astaneh S. The effect of Mentha spicata and Eucalyptus camaldulensis essential oils on dental biofilm. Int J Dent Hyg. 2009;7(3):196\u2013203.","journal-title":"Int J Dent Hyg"},{"issue":"1","key":"5007_CR51","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1186\/s12906-017-1900-3","volume":"17","author":"L Heyman","year":"2017","unstructured":"Heyman L, Houri-Haddad Y, Heyman SN, Ginsburg I, Gleitman Y, Feuerstein O. Combined antioxidant effects of Neem extract, bacteria, red blood cells and lysozyme: possible relation to periodontal disease. BMC Complement Altern Med. 2017;17(1):399.","journal-title":"BMC Complement Altern Med"},{"issue":"1","key":"5007_CR52","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1128\/iai.19.1.254-264.1978","volume":"19","author":"J Slots","year":"1978","unstructured":"Slots J, Gibbons RJ. Attachment of Bacteroides melaninogenicus subsp. Asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect Immun. 1978;19(1):254\u201364.","journal-title":"Infect Immun"},{"issue":"1","key":"5007_CR53","doi-asserted-by":"publisher","first-page":"18313","DOI":"10.1038\/s41598-020-74977-y","volume":"10","author":"Z He","year":"2020","unstructured":"He Z, Zhang X, Song Z, Li L, Chang H, Li S, Zhou W. Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease. Sci Rep. 2020;10(1):18313.","journal-title":"Sci Rep"},{"issue":"9","key":"5007_CR54","doi-asserted-by":"publisher","first-page":"e0185027","DOI":"10.1371\/journal.pone.0185027","volume":"12","author":"K Fujise","year":"2017","unstructured":"Fujise K, Kikuchi Y, Kokubu E, Okamoto-Shibayama K, Ishihara K. Effect of extracytoplasmic function Sigma factors on autoaggregation, hemagglutination, and cell surface properties of Porphyromonas gingivalis. PLoS ONE. 2017;12(9):e0185027.","journal-title":"PLoS ONE"},{"issue":"2","key":"5007_CR55","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.peptides.2012.03.017","volume":"35","author":"M Abdolhosseini","year":"2012","unstructured":"Abdolhosseini M, Nandula SR, Song J, Hirt H, Gorr SU. Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activity. Peptides. 2012;35(2):231\u20138.","journal-title":"Peptides"},{"key":"5007_CR56","doi-asserted-by":"crossref","unstructured":"Selvaraj A, Jayasree T, Valliammai A, Pandian SK. Myrtenol attenuates MRSA biofilm and virulence by suppressing SarA expression dynamism. Front Microbiol. 2019;10.","DOI":"10.3389\/fmicb.2019.02027"},{"issue":"2","key":"5007_CR57","doi-asserted-by":"publisher","first-page":"159","DOI":"10.3109\/10408419109113512","volume":"18","author":"M Rosenberg","year":"1991","unstructured":"Rosenberg M. Basic and applied aspects of microbial adhesion at the hydrocarbon:water interface. Crit Rev Microbiol. 1991;18(2):159\u201373.","journal-title":"Crit Rev Microbiol"},{"key":"5007_CR58","doi-asserted-by":"publisher","first-page":"111570","DOI":"10.1016\/j.colsurfb.2021.111570","volume":"200","author":"NG Fischer","year":"2021","unstructured":"Fischer NG, Aparicio C. The salivary pellicle on dental biomaterials. Colloids Surf B Biointerfaces. 2021;200:111570.","journal-title":"Colloids Surf B Biointerfaces"},{"key":"5007_CR59","doi-asserted-by":"crossref","unstructured":"Papaioannou W, Panagopoulos A, Koletsi-Kounari H, Kontou E, Makou M. Adhesion of Porphyromonas gingivalis and biofilm formation on different types of orthodontic brackets. Int J Dent. 2012; 2012:471380.","DOI":"10.1155\/2012\/471380"},{"issue":"1","key":"5007_CR60","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1038\/s41401-019-0228-6","volume":"41","author":"Y Liu","year":"2020","unstructured":"Liu Y, Grimm M, Dai W-t, Hou M-c, Xiao Z-X, Cao Y. CB-Dock: a web server for cavity detection-guided protein\u2013ligand blind Docking. Acta Pharmacol Sin. 2020;41(1):138\u201344.","journal-title":"Acta Pharmacol Sin"},{"issue":"1","key":"5007_CR61","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1186\/s12906-022-03800-0","volume":"22","author":"M Wei","year":"2022","unstructured":"Wei M, Liu F, Raka RN, Xiang J, Xiao J, Han T, Guo F, Yang S, Wu H. In vitro and in Silico analysis of \u2018taikong blue\u2019 lavender essential oil in LPS-induced HaCaT cells and RAW264.7 murine macrophages. BMC Complement Med Ther. 2022;22(1):324.","journal-title":"BMC Complement Med Ther"},{"key":"5007_CR62","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/B978-0-323-44329-6.00002-4","volume-title":"Equine internal medicine (Fourth Edition)","author":"JL Davis","year":"2018","unstructured":"Davis JL. Chapter 2 - Pharmacologic principles. In: Reed SM, Bayly WM, Sellon DC, editors. Equine internal medicine (Fourth Edition). Missouri: Saunders; 2018. pp. 79\u2013137."},{"issue":"1","key":"5007_CR63","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/omi.12149","volume":"32","author":"JW Smalley","year":"2017","unstructured":"Smalley JW, Olczak T. Heme acquisition mechanisms of Porphyromonas gingivalis \u2013 strategies used in a polymicrobial community in a heme-limited host environment. Mol Oral Microbiol. 2017;32(1):1\u201323.","journal-title":"Mol Oral Microbiol"},{"issue":"4","key":"5007_CR64","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1016\/j.eujim.2016.02.011","volume":"8","author":"FJ Barros","year":"2016","unstructured":"Barros FJ, Costa RJO, Ces\u00e1rio FRAS, Rodrigues LB, da Costa JGM, Coutinho HDM, Galvao HBF, de Menezes IRA. Activity of essential oils of Piper aduncum Anf and Cinnamomum zeylanicum by evaluating osmotic and morphologic fragility of erythrocytes. Eur J Integr Med. 2016;8(4):505\u201312.","journal-title":"Eur J Integr Med"},{"issue":"2","key":"5007_CR65","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1111\/j.1742-7843.2008.00354.x","volume":"104","author":"J He","year":"2009","unstructured":"He J, Lin J, Li J, Zhang J-H, Sun X-M, Zeng C-M. Dual effects of Ginkgo biloba leaf extract on human red blood cells. Basic Clin Pharmacol Toxicol. 2009;104(2):138\u201344.","journal-title":"Basic Clin Pharmacol Toxicol"},{"issue":"1","key":"5007_CR66","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1111\/j.1600-0757.2009.00329.x","volume":"52","author":"JP Lewis","year":"2010","unstructured":"Lewis JP. Metal uptake in host-pathogen interactions: role of iron in Porphyromonas gingivalis interactions with host organisms. Periodontol 2000. 2010;52(1):94\u2013116.","journal-title":"Periodontol 2000"},{"key":"5007_CR67","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/B978-0-443-19143-5.00038-4","volume-title":"Recent frontiers of phytochemicals","author":"I Mohanty","year":"2023","unstructured":"Mohanty I, Mishra R, Panda AK, Mahanty A, Bisht SS. Chapter 26 - Phytochemicals in downregulation of quorum sensing. In: Pati S, Sarkar T, Lahiri D, editors. Recent frontiers of phytochemicals. San Diego: Academic; 2023. pp. 455\u201362."},{"issue":"1","key":"5007_CR68","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.femsre.2004.09.001","volume":"29","author":"T Olczak","year":"2005","unstructured":"Olczak T, Simpson W, Liu X, Genco CA. Iron and Heme utilization in Porphyromonas gingivalis. FEMS Microbiol Rev. 2005;29(1):119\u201344.","journal-title":"FEMS Microbiol Rev"},{"key":"5007_CR69","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.jdsr.2021.09.003","volume":"57","author":"Y Hasegawa","year":"2021","unstructured":"Hasegawa Y, Nagano K. Porphyromonas gingivalis FimA and Mfa1 fimbriae: current insights on localization, function, biogenesis, and genotype. Jpn Dent Sci Rev. 2021;57:190\u2013200.","journal-title":"Jpn Dent Sci Rev"},{"issue":"4","key":"5007_CR70","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1016\/j.foodchem.2009.01.086","volume":"115","author":"E-Y Jeong","year":"2009","unstructured":"Jeong E-Y, Jeon J-H, Kim H-W, Kim M-G, Lee H-S. Antimicrobial activity of leptospermone and its derivatives against human intestinal bacteria. Food Chem. 2009;115(4):1401\u20134.","journal-title":"Food Chem"},{"issue":"2","key":"5007_CR71","doi-asserted-by":"publisher","first-page":"392","DOI":"10.15835\/nbha4229645","volume":"42","author":"A Weso\u0141Owska","year":"2014","unstructured":"Weso\u0141Owska A, Grzeszczuk M, Jadczak D. Influence of harvest term on the content of carvacrol, p-cymene, \u03b3-terpinene and \u03b2-caryophyllene in the essential oil of Satureja montana. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2014;42(2):392\u20137.","journal-title":"Notulae Botanicae Horti Agrobotanici Cluj-Napoca"},{"issue":"2","key":"5007_CR72","doi-asserted-by":"publisher","first-page":"e0229076","DOI":"10.1371\/journal.pone.0229076","volume":"15","author":"EJ Muturi","year":"2020","unstructured":"Muturi EJ, Selling GW, Doll KM, Hay WT, Ramirez JL. Leptospermum scoparium essential oil is a promising source of mosquito larvicide and its toxicity is enhanced by a biobased emulsifier. PLoS ONE. 2020;15(2):e0229076.","journal-title":"PLoS ONE"},{"issue":"1","key":"5007_CR73","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1186\/s12906-025-04834-w","volume":"25","author":"FR Maybodi","year":"2025","unstructured":"Maybodi FR, Herandi V, Vaezpour MS. Effect of aromatherapy with Lemongrass (Cymbopogon citratus) on the anxiety of patients undergoing scaling and root planning: a randomized clinical trial. BMC Complement Med Ther. 2025;25(1):100.","journal-title":"BMC Complement Med Ther"},{"issue":"1","key":"5007_CR74","doi-asserted-by":"publisher","first-page":"14143","DOI":"10.1038\/s41598-024-63657-w","volume":"14","author":"J Czakert","year":"2024","unstructured":"Czakert J, Kandil FI, Boujnah H, Tavakolian P, Blakeslee SB, Stritter W, Dommisch H, Seifert G. Scenting serenity: influence of essential-oil vaporization on dental anxiety - a cluster-randomized, controlled, single-blinded study (AROMA_dent). Sci Rep. 2024;14(1):14143.","journal-title":"Sci Rep"},{"issue":"10","key":"5007_CR75","doi-asserted-by":"publisher","first-page":"1935","DOI":"10.1902\/jop.2007.070120","volume":"78","author":"DH Fine","year":"2007","unstructured":"Fine DH, Markowitz K, Furgang D, Goldsmith D, Ricci-Nittel D, Charles CH, Peng P, Lynch MC. Effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens. J Periodontol. 2007;78(10):1935\u201342.","journal-title":"J Periodontol"},{"key":"5007_CR76","doi-asserted-by":"crossref","unstructured":"Morozumi T, Kubota T, Abe D, Shimizu T, Nohno K, Yoshie H. Microbiological effect of essential oils in combination with subgingival ultrasonic instrumentation and mouth rinsing in chronic periodontitis patients. Int J Dent. 2013; 2013:146479.","DOI":"10.1155\/2013\/146479"},{"key":"5007_CR77","doi-asserted-by":"crossref","unstructured":"Smalley J, Olczak T. Haem acquisition mechanisms of Porphyromonas gingivalis - strategies used in polymicrobial community in a haem-limited host environment. Mol Oral Microbiol. 2015;32.","DOI":"10.1111\/omi.12149"},{"issue":"3","key":"5007_CR78","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1128\/JB.01270-08","volume":"191","author":"SG Dashper","year":"2009","unstructured":"Dashper SG, Ang CS, Veith PD, Mitchell HL, Lo AW, Seers CA, Walsh KA, Slakeski N, Chen D, Lissel JP, et al. Response of Porphyromonas gingivalis to Heme limitation in continuous culture. J Bacteriol. 2009;191(3):1044\u201355.","journal-title":"J Bacteriol"},{"key":"5007_CR79","doi-asserted-by":"crossref","unstructured":"M\u0105czka W, Twardawska M, Grabarczyk M, Wi\u0144ska K. Carvacrol- a natural phenolic compound with antimicrobial properties. Antibiot (Basel). 2023; 12(5).","DOI":"10.3390\/antibiotics12050824"},{"issue":"1","key":"5007_CR80","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1038\/s41598-020-79713-0","volume":"11","author":"NM Wijesundara","year":"2021","unstructured":"Wijesundara NM, Lee SF, Cheng Z, Davidson R, Rupasinghe HPV. Carvacrol exhibits rapid bactericidal activity against Streptococcus pyogenes through cell membrane damage. Sci Rep. 2021;11(1):1487.","journal-title":"Sci Rep"},{"issue":"5","key":"5007_CR81","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1016\/j.jbiosc.2010.06.010","volume":"110","author":"P Rattanachaikunsopon","year":"2010","unstructured":"Rattanachaikunsopon P, Phumkhachorn P. Assessment of factors influencing antimicrobial activity of carvacrol and Cymene against Vibrio cholerae in food. J Biosci Bioeng. 2010;110(5):614\u20139.","journal-title":"J Biosci Bioeng"},{"issue":"1","key":"5007_CR82","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/S0740-0020(03)00046-7","volume":"21","author":"CF Bagamboula","year":"2004","unstructured":"Bagamboula CF, Uyttendaele M, Debevere J. Inhibitory effect of thyme and Basil essential oils, carvacrol, thymol, estragol, Linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol. 2004;21(1):33\u201342.","journal-title":"Food Microbiol"},{"issue":"1","key":"5007_CR83","doi-asserted-by":"publisher","first-page":"4417","DOI":"10.1038\/s41598-022-08217-w","volume":"12","author":"LGV Sousa","year":"2022","unstructured":"Sousa LGV, Castro J, Cavaleiro C, Salgueiro L, Tom\u00e1s M, Palmeira-Oliveira R, Martinez-Oliveira J, Cerca N. Synergistic effects of carvacrol, \u03b1-terpinene, \u03b3-terpinene, \u03c1-cymene and Linalool against Gardnerella species. Sci Rep. 2022;12(1):4417.","journal-title":"Sci Rep"},{"issue":"14","key":"5007_CR84","doi-asserted-by":"publisher","first-page":"4484","DOI":"10.1128\/AEM.00340-07","volume":"73","author":"SA Burt","year":"2007","unstructured":"Burt SA, van der Zee R, Koets AP, de Graaff AM, van Knapen F, Gaastra W, Haagsman HP, Veldhuizen EJ. Carvacrol induces heat shock protein 60 and inhibits synthesis of Flagellin in Escherichia coli O157:H7. Appl Environ Microbiol. 2007;73(14):4484\u201390.","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"5007_CR85","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1128\/AEM.68.4.1561-1568.2002","volume":"68","author":"A Ultee","year":"2002","unstructured":"Ultee A, Bennik MH, Moezelaar R. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol. 2002;68(4):1561\u20138.","journal-title":"Appl Environ Microbiol"},{"key":"5007_CR86","doi-asserted-by":"crossref","unstructured":"Miladi H, Zmantar T, Kouidhi B, Al Qurashi Y, Bakhrouf A, Chaabouni Y, Kacem M, Chaieb K. Synergistic effect of eugenol, carvacrol, thymol, p-cymene and \u03b3-terpinene on Inhibition of drug resistance and biofilm formation of oral bacteria. Microb Pathog. 2017;112.","DOI":"10.1016\/j.micpath.2017.09.057"},{"key":"5007_CR87","unstructured":"Alnaimat S, Wainwright M, Jaber S, Amasha R. Mechanism of the antibacterial action of (Leptospermum scoparium) oil on methicillin-resistant Staphylococcus aureus (MRSA)and E. coli. The 2nd Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-2). Antalya, Turkey. 2015."},{"issue":"6","key":"5007_CR88","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1055\/s-2000-8604","volume":"66","author":"F Christoph","year":"2000","unstructured":"Christoph F, Kaulfers PM, Stahl-Biskup E. A comparative study of the in vitro antimicrobial activity of tea tree oils s.l. with special reference to the activity of beta-triketones. Planta Med. 2000;66(6):556\u201360.","journal-title":"Planta Med"},{"key":"5007_CR89","doi-asserted-by":"publisher","first-page":"106401","DOI":"10.1016\/j.pestbp.2025.106401","volume":"210","author":"S McComic","year":"2025","unstructured":"McComic S, Chen R, Mishra S, Geldenhuys W, Cantrell C, Burgess E, Anderson T, Swale D. Mode of toxicity of the \u03b2-triketone leptospermone to Aedes aegypti mosquitoes. Pestic Biochem Physiol. 2025;210:106401.","journal-title":"Pestic Biochem Physiol"}],"container-title":["BMC Complementary Medicine and Therapies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12906-025-05007-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12906-025-05007-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12906-025-05007-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T19:21:53Z","timestamp":1757272913000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmccomplementalternmed.biomedcentral.com\/articles\/10.1186\/s12906-025-05007-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,22]]},"references-count":89,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["5007"],"URL":"https:\/\/doi.org\/10.1186\/s12906-025-05007-5","relation":{},"ISSN":["2662-7671"],"issn-type":[{"value":"2662-7671","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,22]]},"assertion":[{"value":"31 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"N\/A. This study strictly complied with relevant laws and regulations of the People\u2019s Republic of China, including the Biosecurity Law of the People\u2019s Republic of China, the Regulations on the Administration of Human Genetic Resources, the Regulations on the Bio-safety Management of Pathogenic Microbe Labs, and the Regulations on the Management of Medical Waste, in the acquisition, transportation, storage, and use of biological samples. The collected saliva samples only served as a substrate material to promote bacterial adhesion within the scope of this study. From acquisition to use, these samples posed no harm to human health, involved no sensitive personal information, and were unrelated to any commercial interests. It has been examined and evaluated by the Ethical Review Board of the Sixth Medical Center of the People\u2019s Liberation Army General Hospital, and the ethical review has been waived. Informed consent was obtained from all individual participants involved in the study accordance with the Regulations on the Administration of Human Genetic Resources and the Ethical Review Measures of Life Science and Medical Research Involving Human Participants. All the study procedures adhered to the tenets of the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"N\/A.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"283"}}