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Albendazole (ALB), the nowadays anti-trichinellosis drug, has side effects and insufficient effectiveness.\n                      <jats:italic>Ficus<\/jats:italic>\n                      species have ubiquitous health benefits. The current work aimed to assess the in vitro anti-trichinellosis potential of seven\n                      <jats:italic>Ficus<\/jats:italic>\n                      species compared to the reference drug, ALB.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      Fourteen crude extracts from seven\n                      <jats:italic>Ficus<\/jats:italic>\n                      species (spp.) leaves were prepared using 70% methanol and aqueous solvents.\n                      <jats:italic>Trichinella spiralis<\/jats:italic>\n                      (\n                      <jats:italic>T. spiralis<\/jats:italic>\n                      ) adult worms were isolated from infected mice, cultivated on a 24-well tissue culture plate, and subjected to the crude\n                      <jats:italic>Ficus<\/jats:italic>\n                      spp. extracts and ALB in culture media. The efficacy of the tested compounds was evaluated using parasitological analysis and electron microscopy (SEM). Preliminary phytochemical screening and total phenolic content were performed on\n                      <jats:italic>Ficus<\/jats:italic>\n                      spp. extracts showing the most promising effects. In addition, GC-MS analysis was performed on the most active extract to determine its chemical composition.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The parasitological analysis revealed that\n                      <jats:italic>Ficus microcarpa<\/jats:italic>\n                      (\n                      <jats:italic>F.m<\/jats:italic>\n                      ) had the most promising effects, followed by\n                      <jats:italic>Ficus sycomorus<\/jats:italic>\n                      (\n                      <jats:italic>F.s<\/jats:italic>\n                      ) and\n                      <jats:italic>Ficus nitida<\/jats:italic>\n                      (\n                      <jats:italic>F.n<\/jats:italic>\n                      ) with the methanolic extracts\u2019 LC\n                      <jats:sub>50<\/jats:sub>\n                      of 12.8\u00a0\u00b5g\/mL, 31.5\u00a0\u00b5g\/mL, and 42.9\u00a0\u00b5g\/mL, respectively, compared to the ALB (52.5\u00a0\u00b5g\/mL). SEM results documented changes and a loss of the typical tegumental structure and morphology in adult\n                      <jats:italic>T. spiralis<\/jats:italic>\n                      worms. The GC-MS analysis of\n                      <jats:italic>F.m<\/jats:italic>\n                      methanolic extract yielded 26 compounds, including fatty acids, esters, tricyclic diol, and glucosinolates. Molecular docking studies further supported these results, with high binding affinities among the major compounds of\n                      <jats:italic>F.m<\/jats:italic>\n                      and the tubulin beta chain of\n                      <jats:italic>T. spiralis<\/jats:italic>\n                      , an essential protein for parasite survival.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The current study emphasizes the promising potential of\n                      <jats:italic>Ficus<\/jats:italic>\n                      species, especially\n                      <jats:italic>F.m<\/jats:italic>\n                      , as natural sources for the development of antiparasitic agents for the therapy of trichinellosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12906-026-05500-5","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:14:17Z","timestamp":1786680857000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical investigation and anti-trichinellosis activity of some ficus species leaves: in silico supported in vitro study"],"prefix":"10.1186","volume":"26","author":[{"given":"El-Sayed S.","family":"Abdel-Hameed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezzat E. 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