{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:11:16Z","timestamp":1777702276692,"version":"3.51.4"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Zoological Gardens of South Africa"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s12917-017-1261-7","type":"journal-article","created":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T19:55:44Z","timestamp":1512676544000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In silico and phylogenetic analyses of partial BbRAP-1, BbCP2, BbSBP-4 and Bb\u03b2TUB gene sequences of Babesia bovis isolates from cattle in South Africa"],"prefix":"10.1186","volume":"13","author":[{"given":"Phillip Senzo","family":"Mtshali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moses Sibusiso","family":"Mtshali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,12,8]]},"reference":[{"key":"1261_CR1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1017\/S0031182004005967","volume":"129","author":"F Jongejan","year":"2004","unstructured":"Jongejan F, Uilenberg G. The global importance of ticks. Parasitology. 2004;129:S3\u201314.","journal-title":"Parasitology"},{"key":"1261_CR2","first-page":"278","volume-title":"Infectious diseases of livestock","author":"AJ de Vos","year":"1994","unstructured":"de Vos AJ, Potgieter FT. Bovine babesiosis. In: JAW C, Thomson GR, Tustin RC, editors. Infectious diseases of livestock. South Africa: Oxford University Press; 1994. p. 278\u201394."},{"key":"1261_CR3","unstructured":"OIE. Bovine babesiosis: OIE Terrestrial Manual [Internet]. Available from: \nhttp:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/2.04.02_bovine_babesiosis.pdf\n\n (2010). Accessed 29 Aug 2013."},{"key":"1261_CR4","doi-asserted-by":"crossref","first-page":"S247","DOI":"10.1017\/S0031182004005190","volume":"129","author":"R Bock","year":"2004","unstructured":"Bock R, Jackson L, de Vos AJ, Jorgensen W. Babesiosis of cattle. Parasitology. 2004;129:S247\u201369.","journal-title":"Parasitology"},{"key":"1261_CR5","first-page":"443","volume-title":"Disease of cattle in the tropics","author":"M Ristic","year":"1981","unstructured":"Ristic M. Babesiosis. In: Ristic M, McIntyre I, editors. Disease of cattle in the tropics. The Netherlands: Martinus Nijhof Publishers; 1981. p. 443\u201368."},{"key":"1261_CR6","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1749-6632.2000.tb05333.x","volume":"916","author":"A de Vos","year":"2000","unstructured":"de Vos A, Bock RE. Vaccination against bovine babesiosis. Ann N Y Acad Sci. 2000;916:540\u20135.","journal-title":"Ann N Y Acad Sci"},{"issue":"1\u20132","key":"1261_CR7","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1865-1682.2010.01123.x","volume":"57","author":"JJ Ojeda","year":"2010","unstructured":"Ojeda JJ, Orozco L, Flores R, Figueroa JV, Alvarez JA. Validation of an attenuated live vaccine against babesiosis in native cattle in an endemic area. Transbound Emerg Dis. 2010;57(1\u20132):84\u20136.","journal-title":"Transbound Emerg Dis"},{"issue":"7","key":"1261_CR8","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/S0020-7519(97)00034-9","volume":"27","author":"LL Callow","year":"1997","unstructured":"Callow LL, Dalgliesh RJ, de Vos AJ. Development of effective living vaccines against bovine babesiosis: the longest field trial? Int J Parasitol. 1997;27(7):747\u201367.","journal-title":"Int J Parasitol"},{"issue":"1\u20132","key":"1261_CR9","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1865-1682.2010.01118.x","volume":"57","author":"A Torina","year":"2010","unstructured":"Torina A, Agnone A, Sireci G, Mosqueda JJ, Blanda V, Albanese I, La Farina M, Cerrone A, Cusumano F, Caracappa S. Characterization of the apical membrane antigen-1 in Italian strains of Babesia bigemina. Transbound Emerg Dis. 2010;57(1\u20132):52\u20136.","journal-title":"Transbound Emerg Dis"},{"issue":"3\u20134","key":"1261_CR10","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.vetpar.2012.01.020","volume":"187","author":"CAN Ramos","year":"2012","unstructured":"Ramos CAN, Ara\u00fajo FR, Alves LC, de Souza IIF, Guedes DS Jr, Soares CO. Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis. Vet Parasitol. 2012;187(3\u20134):548\u201352.","journal-title":"Vet Parasitol"},{"issue":"10","key":"1261_CR11","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1371\/journal.ppat.0030148","volume":"3","author":"KA Brayton","year":"2007","unstructured":"Brayton KA, Lau AOT, Herndon DR, Hannick L, Kappmeyer LS, Berens SJ, Bidwell SL, Brown W, Crabtree J, Fadrosh D, Feldblum T, Forberger HA, Haas BJ, Howell JM, Khouri H, Koo H, Mann DJ, Norimine J, Paulsen IT, Radune D, Nene VM. Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa. PLoS Pathog. 2007;3(10):1401\u201313.","journal-title":"PLoS Pathog"},{"issue":"1\u20132","key":"1261_CR12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0304-4017(93)90008-B","volume":"50","author":"JV Figueroa","year":"1993","unstructured":"Figueroa JV, Chieves LP, Johnson GS, Buening GM. Multiplex polymerase chain reaction based assay for the detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood. Vet Parasitol. 1993;50(1\u20132):69\u201381.","journal-title":"Vet Parasitol"},{"key":"1261_CR13","first-page":"95","volume":"41","author":"TA Hall","year":"1999","unstructured":"Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95\/98\/NT. Nucleic Acids Symp Ser. 1999;41:95\u20138.","journal-title":"Nucleic Acids Symp Ser"},{"issue":"10","key":"1261_CR14","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","volume":"28","author":"K Tamura","year":"2011","unstructured":"Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731\u20139.","journal-title":"Mol Biol Evol"},{"issue":"2","key":"1261_CR15","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF01731581","volume":"16","author":"M Kimura","year":"1980","unstructured":"Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111\u201320.","journal-title":"J Mol Evol"},{"issue":"4","key":"1261_CR16","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","volume":"39","author":"J Felsenstein","year":"1985","unstructured":"Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39(4):783\u201391.","journal-title":"Evolution"},{"issue":"1\u20132","key":"1261_CR17","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.vetpar.2006.01.037","volume":"138","author":"N Yokoyama","year":"2006","unstructured":"Yokoyama N, Okamura M, Igarashi I. Erythrocyte invasion by Babesia parasites: current advances in the elucidation of the molecular interactions between the protozoan ligands and host receptors in the invasion stage. Vet Parasitol. 2006;138(1\u20132):22\u201332.","journal-title":"Vet Parasitol"},{"key":"1261_CR18","first-page":"9","volume":"1","author":"M Florin-Christensen","year":"2007","unstructured":"Florin-Christensen M, Schnittger L, Dominguez M, Mesplet M, Rodriguez A, Ferreri L, Asenzo G, Wilkowsky S, Farber M, Echaide I, Suarez C. Search for Babesia bovis vaccine candidates. Parassitologia. 2007;1:9\u201312.","journal-title":"Parassitologia"},{"issue":"2","key":"1261_CR19","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1006\/expr.1998.4321","volume":"90","author":"CE Suarez","year":"1998","unstructured":"Suarez CE, Palmer GH, H\u04e7tzel I, Hines SA, McElwain TF. Sequence and functional analysis of the intergenic regions separating babesial rhoptry-associated protein-1 (rap-1) genes. Exp Parasitol. 1998;90(2):189\u201394.","journal-title":"Exp Parasitol"},{"issue":"1","key":"1261_CR20","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.molbiopara.2004.02.006","volume":"136","author":"FR Gaffar","year":"2004","unstructured":"Gaffar FR, Yatsuda AP, Franssen FF, Vries EA. A Babesia bovis merozoite protein with a domain architecture highly similar to the thrombospondin-related anonymous protein (TRAP) present in Plasmodium sporozoites. Mol Biochem Parasitol. 2004;136(1):25\u201334.","journal-title":"Mol Biochem Parasitol"},{"issue":"2\u20132","key":"1261_CR21","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.molbiopara.2011.06.005","volume":"179","author":"M Mesplet","year":"2011","unstructured":"Mesplet M, Palmer GH, Pedroni MJ, Echaide I, Florin-Christensen M, Schnittger L, Lau AOT. Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair. Mol Biochem Parasitol. 2011;179(2\u20132):111\u20133.","journal-title":"Mol Biochem Parasitol"},{"issue":"4","key":"1261_CR22","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.exppara.2007.11.010","volume":"118","author":"YK Goo","year":"2008","unstructured":"Goo YK, Jia H, Aboge GO, Terkawi MA, Kuriki K, Nakamura C, Kumagai A, Zhou J, Lee EG, Nishikawa Y, Igarashi I, Fujisaki K, Xuan X. Babesia gibsoni: serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP. Exp Parasitol. 2008;118(4):555\u201360.","journal-title":"Exp Parasitol"},{"issue":"2","key":"1261_CR23","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1128\/CVI.00388-10","volume":"18","author":"MA Terkawi","year":"2011","unstructured":"Terkawi MA, Huyen NX, Wibowo PE, Seuseu FJ, AbouLaila M, Ueno A, Goo YK, Yokoyama N, Xuan X, Igarashi I. Spherical body protein 4 is a new serological antigen for global detection of Babesia bovis infection in cattle. Clin Vaccine Immunol. 2011;18(2):337\u201342.","journal-title":"Clin Vaccine Immunol"},{"issue":"11","key":"1261_CR24","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S0020-7519(00)00105-3","volume":"30","author":"S Cacci\u00f2","year":"2000","unstructured":"Cacci\u00f2 S, Camm\u00e0 C, Onuma M, Severini C. The \u03b2-tubulin gene of Babesia and Theileria parasites is an informative marker for species discrimination. Int J Parasitol. 2000;30(11):1181\u20135.","journal-title":"Int J Parasitol"},{"issue":"11","key":"1261_CR25","doi-asserted-by":"crossref","first-page":"7180","DOI":"10.1128\/IAI.73.11.7180-7189.2005","volume":"73","author":"SJ Berens","year":"2005","unstructured":"Berens SJ, Brayton KA, Molloy JB, Bock RE, Lew AE, McElwain TF. Merozoite surface antigen 2 proteins of Babesia bovis vaccine breakthrough isolates contain a unique hypervariable region composed of degenerate repeats. Infect Immun. 2005;73(11):7180\u20139.","journal-title":"Infect Immun"},{"issue":"4","key":"1261_CR26","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1128\/IAI.70.4.2039-2048.2002","volume":"70","author":"J Norimine","year":"2002","unstructured":"Norimine J, Suarez CE, McElwain TF, Florin-Christensen M, Brown WC. Immunodominant epitopes in Babesia bovis rhoptry-associated protein 1 that elicit memory CD4+-T-lymphocyte responses in B. bovis-immune individuals are located in the amino-terminal domain. Infect Immun. 2002;70(4):2039\u201348.","journal-title":"Infect Immun"},{"issue":"1\u20132","key":"1261_CR27","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.vetpar.2010.04.011","volume":"172","author":"M AbouLaila","year":"2010","unstructured":"AbouLaila M, Yokoyama N, Igarashi I. Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood. Vet Parasitol. 2010;172(1\u20132):65\u201370.","journal-title":"Vet Parasitol"},{"issue":"1\u20132","key":"1261_CR28","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.vetpar.2009.07.031","volume":"166","author":"MG Silva","year":"2009","unstructured":"Silva MG, Henriques G, S\u00e1nchez C, Marques PX, Suarez CE, Oova A. First survey for Babesia bovis and Babesia bigemina infection in cattle from central and southern regions of Portugal using serological and DNA detection methods. Vet Parasitol. 2009;166(1\u20132):66\u201372.","journal-title":"Vet Parasitol"},{"key":"1261_CR29","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/1756-3305-3-113","volume":"3","author":"M Mesplet","year":"2010","unstructured":"Mesplet M, Echaide I, Dominguez M, Mosqueda JJ, Suarez CE, Schnittger L, Florin-Christensen M. Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis. Parasit Vectors. 2010;3:113.","journal-title":"Parasit Vectors"},{"issue":"2","key":"1261_CR30","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.exppara.2007.04.009","volume":"117","author":"K Okubo","year":"2007","unstructured":"Okubo K, Yokoyama N, Govind Y, Alhassan A, Igarashi I. Babesia bovis: effects of cysteine protease inhibitors on in vitro growth. Exp Parasitol. 2007;117(2):214\u20137.","journal-title":"Exp Parasitol"},{"issue":"1","key":"1261_CR31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-6851(99)00167-X","volume":"105","author":"BJ Ruef","year":"2000","unstructured":"Ruef BJ, Dowling SC, Conley PG, Perryman LE, Brown WC, Jasmer DP, Rice-Ficht AC. A unique Babesia bovis spherical body protein is conserved among geographical isolates and localizes to the infected erythrocyte membrane. Mol Biochem Parasitol. 2000;105(1):1\u201312.","journal-title":"Mol Biochem Parasitol"},{"issue":"1\u20132","key":"1261_CR32","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.molbiopara.2011.02.006","volume":"178","author":"MA Terkawi","year":"2011","unstructured":"Terkawi MA, Seuseu FJ, Eko-Wibowo P, Huyen NX, Minoda Y, AbouLaila M, Kawai S, Yokoyama N, Xuan X, Igarashi I. Secretion of a new spherical body protein of Babesia bovis into the cytoplasm of infected erythrocytes. Mol Biochem Parasitol. 2011;178(1\u20132):40\u20135.","journal-title":"Mol Biochem Parasitol"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-017-1261-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T19:56:02Z","timestamp":1512676562000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-017-1261-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["1261"],"URL":"https:\/\/doi.org\/10.1186\/s12917-017-1261-7","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"article-number":"383"}}