{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T22:41:24Z","timestamp":1771540884863,"version":"3.50.1"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T00:00:00Z","timestamp":1547769600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National key Research and Development Program of China","award":["2016YFD0500600"],"award-info":[{"award-number":["2016YFD0500600"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s12917-019-1773-4","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T14:04:01Z","timestamp":1547820241000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay"],"prefix":"10.1186","volume":"15","author":[{"given":"Hongfen","family":"Bian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3070-9518","authenticated-orcid":false,"given":"Fei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumin","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengchao","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiqing","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,1,18]]},"reference":[{"key":"1773_CR1","doi-asserted-by":"crossref","unstructured":"DA B RSB. Coronavirus genome structure and replication. Current TopICA in Microbiology and Immunology. 2005:1\u201330.","DOI":"10.1007\/3-540-26765-4_1"},{"key":"1773_CR2","unstructured":"A B, R K, K T, A C, M. A (1998) Further analysis of the genome of porcine epidemic diarrhoea virus. Adv Exp Med Biol 781\u2013786."},{"key":"1773_CR3","unstructured":"D S, B P (2012) Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes 2:167\u20132175."},{"key":"1773_CR4","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1136\/vr.132.6.129","volume":"6","author":"A Pijpers","year":"1993","unstructured":"Pijpers A, van Nieuwstadt A, Terpstra C, Verheijden J. Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs. Vet Rec. 1993;6:129\u201331.","journal-title":"Vet Rec"},{"key":"1773_CR5","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1136\/vr.100.12.243","volume":"12","author":"E Wood","year":"1977","unstructured":"Wood E. An apparently new syndrome of porcine epidemic diarrhoea. Vet Rec. 1977;12:243\u20132444.","journal-title":"Vet Rec"},{"key":"1773_CR6","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.tvjl.2015.02.017","volume":"2","author":"K Jung","year":"2015","unstructured":"Jung K, Saif LJ. Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet J. 2015;2:134\u201343.","journal-title":"Vet J"},{"issue":"19","key":"1773_CR7","doi-asserted-by":"publisher","first-page":"10910","DOI":"10.1128\/JVI.01919-12","volume":"86","author":"J Bi","year":"2012","unstructured":"Bi J, Zeng S, Xiao S, et al. Complete genome sequence of porcine epidemic diarrhea virus strain AJ1102 isolated from a suckling piglet with acute diarrhea in China[J]. J Virol. 2012;86(19):10910\u20131.","journal-title":"J Virol"},{"issue":"8","key":"1773_CR8","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.3201\/eid1803.120002","volume":"18","author":"W Li","year":"2011","unstructured":"Li W, Li H, Liu Y, et al. New variants of porcine epidemic diarrhea virus. China Emerging infectious diseases. 2011;18(8):1350.","journal-title":"China Emerging infectious diseases"},{"key":"1773_CR9","unstructured":"1 DSS, 1 BKK, 3 JSO, 3 GWH, 2 JSY, 2 HJM, et al (2006) Multiplex reverse transcription-PCR for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group A rotavirus. J Vet Diagn Investig 3:278\u2013281."},{"key":"1773_CR10","doi-asserted-by":"publisher","first-page":"2224","DOI":"10.1021\/acs.analchem.7b04595","volume":"3","author":"X Li","year":"2018","unstructured":"Li X, Bian H, Yu S, Xiao W, Shen J, Lan C, et al. A rapid method for antigen-specific Hybridoma clone isolation. Anal Chem. 2018;3:2224\u20139.","journal-title":"Anal Chem"},{"key":"1773_CR11","first-page":"191","volume":"1\u20132","author":"HS K I","year":"1997","unstructured":"K I HS, T O SS. Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR. J Virol Methods. 1997;1\u20132:191\u20135.","journal-title":"J Virol Methods"},{"key":"1773_CR12","first-page":"313","volume":"2","author":"K K","year":"1992","unstructured":"K K, H K, T K, T N, Y I, T S, et al. Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate. J Vet Med Sci. 1992;2:313\u201321.","journal-title":"J Vet Med Sci"},{"key":"1773_CR13","doi-asserted-by":"crossref","unstructured":"DG1 D, S2 L, F2 O, A2 S, T2 C, MH2 F, et al. Porcine epidemic diarrhea virus: an overview of current virological and serological diagnostic methods. Virus Res. 2016:60\u201370.","DOI":"10.1016\/j.virusres.2016.05.013"},{"key":"1773_CR14","first-page":"9","volume":"1","author":"R Lrvc","year":"2005","unstructured":"Lrvc R, Val\u00ed\u010dek L, \u0160m\u00edd B, Nevor\u00e1nkov\u00e1 Z. An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces. Vet Microbiol. 2005;1:9\u201317.","journal-title":"Vet Microbiol"},{"key":"1773_CR15","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1136\/vr.103959","volume":"181","author":"L KS","year":"2017","unstructured":"KS L, M Y, J K, D K, G H, W N, et al. Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus. Vet Rec. 2017;181:596\u2013601.","journal-title":"Vet Rec"},{"key":"1773_CR16","unstructured":"FX1 W, DY2 Y, YN3 J, L3 H, ZY2 S, Q2 H, et al. Reverse transcription cross-priming amplification-nucleic acid test strip for rapid detection of porcine epidemic diarrhea virus. Sci Rep. 2016:24702."},{"key":"1773_CR17","doi-asserted-by":"crossref","unstructured":"Kim YK, Lim S-I, Cho I-S, Cheong K-M, Lee E-J, Lee S-O, et al. A novel diagnostic approach to detecting porcine epidemic diarrhea virus: the lateral immunochromatography assay. J Virol Methods. 2015:4\u20138.","DOI":"10.1016\/j.jviromet.2015.08.024"},{"key":"1773_CR18","doi-asserted-by":"publisher","first-page":"3704","DOI":"10.1007\/s12274-015-0870-3","volume":"11","author":"L Rivas","year":"2015","unstructured":"Rivas L, Adl E-M, Serrano L, Altet L, Francino O, S\u00e1nchez A, et al. Triple lines gold nanoparticle-based lateral flow assay for enhanced and simultaneous detection of Leishmania DNA and endogenous control. Nano Res. 2015;11:3704\u201314.","journal-title":"Nano Res"},{"key":"1773_CR19","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1007\/s00604-014-1362-y","volume":"3\u20134","author":"Y Man","year":"2015","unstructured":"Man Y, Lv X, Iqbal J, Peng G, Song D, Zhang C, et al. Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor alpha using gold nanoparticles as labels. Microchim Acta. 2015;3\u20134:597\u2013604.","journal-title":"Microchim Acta"},{"key":"1773_CR20","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1007\/s00604-017-2121-7","volume":"5","author":"FD Nardo","year":"2017","unstructured":"Nardo FD, Baggiani C, Giovannoli C, Spano G, Anfossi L. Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins. Microchim Acta. 2017;5:1295\u2013304.","journal-title":"Microchim Acta"},{"issue":"5","key":"1773_CR21","first-page":"269","volume":"13","author":"DS Song","year":"2005","unstructured":"Song DS, Oh JS, Kang BK, et al. Fecal shedding of a highly cell-culture-adapted porcine epidemic diarrhea virus after oral inoculation in pigs [J]. Journal of Swine Health and Production. 2005;13(5):269\u201372.","journal-title":"Journal of Swine Health and Production"},{"issue":"2","key":"1773_CR22","doi-asserted-by":"publisher","first-page":"e00165","DOI":"10.1128\/genomeA.00165-14","volume":"2","author":"A Jia","year":"2014","unstructured":"Jia A, Feng X, Liu Q, et al. Complete genome sequence of CHYJ130330, a highly virulent strain of porcine epidemic diarrhea virus in South China[J]. Genome announcements. 2014;2(2):e00165\u201314.","journal-title":"Genome announcements"},{"key":"1773_CR23","doi-asserted-by":"publisher","first-page":"4393","DOI":"10.1039\/C7AN01227F","volume":"142","author":"S H","year":"2017","unstructured":"H S, F X, M X, Q F, Z C, S Z, et al. A new lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for rapid detection of tetrodotoxin. Analyst. 2017;142:4393\u20138.","journal-title":"Analyst"},{"key":"1773_CR24","doi-asserted-by":"crossref","unstructured":"Xiao M, Fu Q, Shen H, Chen Y, Xiao W, Yan D, et al. A turn-on competitive immunochromatographic strips integrated with quantum dots and gold nano-stars for cadmium ion detection. Talanta. 2018:644\u20139.","DOI":"10.1016\/j.talanta.2017.10.002"},{"key":"1773_CR25","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1007\/s00604-016-2028-8","volume":"3","author":"W Wang","year":"2017","unstructured":"Wang W, Liu L, Song S, Xu L, Kuang H, Zhu J, et al. Identification and quantification of eight Listeria monocytogene serotypes from Listeria spp. using a gold nanoparticle-based lateral flow assay. Microchim Acta. 2017;3:715\u201324.","journal-title":"Microchim Acta"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-019-1773-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12917-019-1773-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-019-1773-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,22]],"date-time":"2020-11-22T22:46:54Z","timestamp":1606085214000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-019-1773-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,18]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["1773"],"URL":"https:\/\/doi.org\/10.1186\/s12917-019-1773-4","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,18]]},"assertion":[{"value":"5 June 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 January 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 January 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All samples were obtained from healthy or naturally infected animals in the field by qualifiers. Therefore, no aggressive operation was conducted against pigs for sampling purpose and no pigs were sacrificed in this study. The study used mice for generation of mAbs and those mice had to be sacrificed. The mice were euthanized by cervical dislocation. Thumb and forefinger were used to press down on the mice\u2019s head. The other hand grabbed the mice\u2019s tail and pulled it slightly back to the top to make the cervical spine disengage. The spinal cord was separated from the brain and the animal died immediately. There was no destruction of brain or section of major blood vessels in the neck during the process. All animal experiments were performed using protocols approved by Laboratory Animal Ethics Committee of Jinan University.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Publisher\u2019s Note"}}],"article-number":"32"}}