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Samples and analyses included in the present study were performed in accordance with current Regulation concerning of Maintenance of Bovine Brucellosis free Health Status (Directive 97\/12\/CE, adopted by Italian Law 196\/199). No experimental protocols were applied; consequently, the need for ethics approval is deemed unnecessary by the IRB of IZSPLV, because the activities described in this study are not part of the pertinence field of current Regulation on the protection of animals used for scientific purposes (Directive 2010\/63\/UE, adopted by Italian Law 26\/2014).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"257"}}