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It is considered as a neglected tropical disease with highest prevalence throughout tropical and subtropical regions, particularly in Africa, Asia, Europe, and Australia. There are few trematocidal drugs available in the market. Most are resistant and have elevated side effects. Therefore, alternative trematocidal drugs need to discover. This study was conducted to evaluate three plants leaf extracts (from<jats:italic>Senna alata<\/jats:italic>,<jats:italic>S. alexandrina,<\/jats:italic>and<jats:italic>S. occidentalis<\/jats:italic>) as effective remedies against gastrointestinal trematode parasite (<jats:italic>Paramphistomum gracile<\/jats:italic>) of ruminants<jats:italic>.<\/jats:italic>Live adult parasites were collected in 0.1\u2009M phosphate-buffered saline (PBS) from fresh autopsied goat\u2019s rumen. Parasites were incubated in leaf extracts of<jats:italic>S. alata<\/jats:italic>,<jats:italic>S. alexandrina<\/jats:italic>and,<jats:italic>S. occidentalis<\/jats:italic>individually and in combination (1:1) ratio at 37\u2009\u00b1\u20091\u00b0C. Treatment media contain extracts at different concentrations (10, 20 and 40\u2009mg\/mL) in 10\u2009mL of 0.1\u2009M PBS with 1% dimethylsulphoxide (DMSO). Parasites in control group were incubated in PBS without extract. The efficacy of three<jats:italic>Senna<\/jats:italic>extracts was evaluated on the basis of dose-dependent motility and mortality of the trematode. Immediately after paralysis, all treated parasites were collected for histology, SEM and biochemical study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Dose-dependent efficacy was observed in terms of motility and time of mortality in all treated parasites after exposure in various concentrations of the<jats:italic>Senna<\/jats:italic>plant extracts.<jats:italic>S. occidentalis<\/jats:italic>and<jats:italic>S. alexandrina<\/jats:italic>showed better efficacy in combination than comparing with individual treatment groups. Histological study and scanning electron microscopic observations revealed conspicuous deformity of surface architecture in all treated parasites. Scanning electron micrographs also revealed shrinkage, vacuolization, infoldings and blebbings on the body surface of treated worms. Activities of tegumental enzymes were inhibited in all treatment groups compared to control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The overall findings from this study revealed that all three<jats:italic>Senna<\/jats:italic>leaf extracts individually and in combination showed potential antitrematocidal activity against<jats:italic>Paramphistomum gracile<\/jats:italic>by damaging body tegument and neural propagation. Thus, this study confirmed that all three<jats:italic>Senna<\/jats:italic>extracts can be considered as a potential drug-like candidate in indigenous system of traditional medicine against trematode infections in livestock.<\/jats:p><\/jats:sec><jats:sec><jats:title>Graphical abstract<\/jats:title><\/jats:sec>","DOI":"10.1186\/s12917-019-2094-3","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T04:28:23Z","timestamp":1571804903000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["An in vitro confirmation of the ethonopharmacological use of Senna plants as anthelmintic against rumen fluke Paramphistomum gracile"],"prefix":"10.1186","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8750-9018","authenticated-orcid":false,"given":"Saptarshi","family":"Roy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larisha Mawkhlieng","family":"Lyndem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,22]]},"reference":[{"key":"2094_CR1","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1016\/0020-7519(91)90150-6","volume":"21","author":"PF Rolfe","year":"1991","unstructured":"Rolfe PF, Boray JC, Nichols P, Collins GH. 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All the animals were stayed at the slaughterhouse in the farm and normally fed was given. Moreover, the owner slough the goat for selling meat in their shop, from there we are only collected the parasite from the rumen. So there is no need for any animals for rearing into the animal house, Hence, no animal ethics approval was needed for this research work.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"360"}}