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Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are <jats:italic>M. bovis<\/jats:italic>-specific. Laboratory diagnosis is therefore important. Serological tests to detect <jats:italic>M. bovis<\/jats:italic> antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and\/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (<jats:italic>Se<\/jats:italic>) and specificity <jats:italic>(Sp)<\/jats:italic> of two commercially available ELISA tests (ID Screen\u00ae ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of <jats:italic>M. bovis<\/jats:italic> infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer\u2019s instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of <jats:italic>Se<\/jats:italic> and <jats:italic>Sp<\/jats:italic> values using latent class analysis.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The <jats:italic>Se<\/jats:italic> of WB, the ID Screen\u00ae ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding <jats:italic>Sp<\/jats:italic> of the three tests were 99.6, 98.6 and 89.6%, respectively.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen\u00ae ELISA. Based on our results, the ID Screen\u00ae ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-019-2117-0","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T18:12:13Z","timestamp":1572027133000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis"],"prefix":"10.1186","volume":"15","author":[{"given":"Anna-Maria","family":"Andersson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Asp\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3747-8054","authenticated-orcid":false,"given":"Henk J.","family":"Wisselink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bregtje","family":"Smid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Ridley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinikka","family":"Pelkonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiina","family":"Autio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klara T\u00f8lb\u00f8ll","family":"Lauritsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Kens\u00f8","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Gaurivaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Tardy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,25]]},"reference":[{"issue":"Suppl 1","key":"2117_CR1","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1111\/tbed.12860","volume":"65","author":"MJ Calcutt","year":"2018","unstructured":"Calcutt MJ, Lysnyansky I, Sachse K, Fox LK, Nicholas RAJ, Ayling RD. 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In Finland, serum samples were submitted for diagnostic purposes to the Finnish Food Authority with a consent from the farmers participating in a research project \u201c<i>Mycoplasma bovis<\/i> in dairy herds\u201d. Samples for the purpose of health screening or disease treatment fall under the Finnish Animal Diseases Act (441\/2013) and do not require further ethical approval, as they are intended to maintain the well-being of animals. In France, serum samples were acquired for diagnosis purpose with a consent from the farmers to reuse them for non-commercial, research activities. Sample collection for diagnosis purpose in France does not require approval by our local ethics committee (Anses\/ENVA\/UPEC). In the Netherlands, serum sampling of the calves was granted an exemption from requiring ethics approval by the institutional Animal Experiment Commission \u201cDier Experimenten Commissie (DEC) Lelystad (2,013,111.b)\u201d, because sampling was performed for diagnostic purposes. Consent for participation was obtained verbally from the farmers. In Sweden, serum sampling of cattle is systematically performed each year for disease screening on behalf of the Swedish Board of Agriculture, and a consent to use a subset of these samples in the present study was given by the Board. Use of such serum samples do not demand ethical permission according to Swedish national law, as stated in the code of statute SJVSF 2019:9 (). This exemption from ethical permission was confirmed by Djurskyddsorganet (the Animal Welfare Body, SVA, Sweden). All Swedish cattle herds are affiliated to a voluntary or mandatory surveillance program, as regulated by SJVFS 1993:42 and SJVFS 2003:31 (), respectively. Consent by each farmer is given by registration to one of these surveillance programs. In the UK, serum samples were submitted to APHA for diagnostic purposes. Samples from veterinary interventions such blood sample collection for the purpose of health screening or disease treatment fall under the Veterinary Surgeons Act 1966 and do not require further ethical approval, as they are intended to maintain the well-being of animals.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"369"}}