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This work aimed to analyse the diversity of staphylococcal species in food-producing animals in Dakar\/Senegal, and to determine the antimicrobial resistance phenotype\/genotype and virulence factors of recovered isolates. Nasal samples of 149 cows and 199 chickens (348 animals) were collected from one slaughterhouse and a local market respectively, and were inoculated on selective media for staphylococci recovery. For <jats:italic>S. aureus<\/jats:italic> isolates, molecular typing (<jats:italic>spa<\/jats:italic>-type, MLST) was performed by PCR\/sequencing, and the presence of 27 virulence genes (exfoliative and toxic shock toxins, PVL, haemolysins and enterotoxins) as well as the gene <jats:italic>scn<\/jats:italic> were analysed by PCR. Susceptibility to twelve antibiotics was studied by disc-diffusion method for all staphylococci; the resistance genes involved were screened by PCR.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>Staphylococcus<\/jats:italic> spp. was present in 3 and 26.8% of chicken and cow nasal samples, respectively. Seven <jats:italic>S. aureus<\/jats:italic> isolates and forty isolates of other staphylococcal species were identified. <jats:italic>S. aureus<\/jats:italic> isolates were recovered from cow (<jats:italic>n<\/jats:italic>\u2009=\u20096) and chicken (<jats:italic>n<\/jats:italic>\u2009=\u20091) samples, belonging to four genetic lineages: t084\/ST15 (<jats:italic>n<\/jats:italic>\u2009=\u20091); t10579\/ST291 (<jats:italic>n<\/jats:italic>\u2009=\u20093); t355, t4690\/ST152 (<jats:italic>n<\/jats:italic>\u2009=\u20092); and t6618\/ST6 (n\u2009=\u20091). All <jats:italic>S. aureus<\/jats:italic> were methicillin-susceptible, penicillin-resistant (<jats:italic>blaZ<\/jats:italic>), and two of them were also tetracycline-resistant [<jats:italic>tet<\/jats:italic>(K)]. All the isolates carried at least one of the virulence genes tested. The PVL genes were detected in three ST15 and ST152 isolates. They all harboured haemolysins encoding genes and lacked the <jats:italic>scn<\/jats:italic> gene. The other staphylococci recovered were <jats:italic>S. sciuri<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u200916), <jats:italic>S. simulans<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u200911), <jats:italic>S. hyicus<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20095), <jats:italic>S. haemolyticus<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20094), <jats:italic>S. chromogenes<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20093), and <jats:italic>S. hominis<\/jats:italic> (n\u2009=\u20091); they were all methicillin-susceptible and 27.5% tetracycline-resistant [<jats:italic>tet<\/jats:italic>(K) and <jats:italic>tet<\/jats:italic>(L)].<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>A low prevalence of <jats:italic>S. aureus<\/jats:italic> was detected among food-producing animals, all susceptible to methicillin. However, the presence of virulence genes (<jats:italic>lukF\/lukS-PV<\/jats:italic>, <jats:italic>eta<\/jats:italic>, <jats:italic>tst<\/jats:italic>, <jats:italic>sea<\/jats:italic> and <jats:italic>see<\/jats:italic>) is worrisome to the extent that they could be transferred to derived food and therefore, to humans.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-019-2137-9","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T14:03:24Z","timestamp":1572876204000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Genetic characterisation of staphylococci of food-producing animals in Senegal. PVL detection among MSSA"],"prefix":"10.1186","volume":"15","author":[{"given":"Olouwafemi Mistourath","family":"Mama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Modou","family":"Dieng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bocar","family":"Hanne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ruiz-Ripa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Codou Gueye Mar","family":"Diop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3709-1690","authenticated-orcid":false,"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,4]]},"reference":[{"key":"2137_CR1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1111\/j.1469-0691.2009.03110.x","volume":"16","author":"JAJW Kluytmans","year":"2010","unstructured":"Kluytmans JAJW. 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No animal was sampled alive or sacrificed for the purpose of this study. Thus, according to the Spanish National law for the protection of animals (RD53\/2013) that transcribes the EU directive 2010\/63\/UE, no ethics committee approval was deemed necessary. Furthermore, ethic committee approval was not deemed necessary based on the Article 7.1 (on the recommendations for animal welfare) and the Article 7.8 (on the use of animals in research and education) of the OIE Terrestrial Animal Health Code followed by Senegal.At the time of processing the entity responsible for the animals was the slaughterhouse and authorization for sample collection was obtained from the directing board of the slaughterhouse. Also, the chicken sampling was authorized verbally by the owner which is responsible for the animals.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"391"}}