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DNA topology is a consolidated target for drug discovery. In this regard, topoisomerase IB \u2013 one of the enzymes controlling DNA topology \u2013 has been poisoned by hundreds of compounds that increase DNA fragility and cell death. Aromathecins are novel molecules with a multiheterocyclic ring scaffold that have higher stability than camptothecins.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Aromathecins showed strong activity against both forms of <jats:italic>L. infantum<\/jats:italic> parasites, free-living promastigotes and intra-macrophagic amastigotes harbored in ex vivo splenic explant cultures obtained from infected BALB\/c mice. However, they prevented the relaxation activity of leishmanial topoisomerase IB weakly, which suggests that the inhibition of topoisomerase IB partially explains the antileishmanial effect of these compounds. The effect of aromathecins was also studied against a strain resistant to camptothecin, and results suggested that the trafficking of these compounds is not through the ABCG6 transporter.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Aromathecins are promising novel compounds against canine leishmaniasis that can circumvent potential resistances based on drug efflux pumps.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12917-019-2153-9","type":"journal-article","created":{"date-parts":[[2019,11,9]],"date-time":"2019-11-09T17:03:13Z","timestamp":1573318993000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antiparasitic effect of synthetic aromathecins on Leishmania infantum"],"prefix":"10.1186","volume":"15","author":[{"given":"Rosa M.","family":"Reguera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"\u00c1lvarez-Velilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara","family":"Dom\u00ednguez-Asenjo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camino","family":"Guti\u00e9rrez-Corbo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Bala\u00f1a-Fouce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Cushman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2361-3785","authenticated-orcid":false,"given":"Yolanda","family":"P\u00e9rez-Pertejo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,9]]},"reference":[{"key":"2153_CR1","doi-asserted-by":"publisher","first-page":"718","DOI":"10.1016\/j.pt.2017.05.005","volume":"33","author":"G Mir\u00f3","year":"2017","unstructured":"Mir\u00f3 G, Petersen C, Cardoso L, Bourdeau P, Baneth G, Solano-Gallego L, Pennisi MG, Ferrer L, Oliva G. 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