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Therefore, the objectives of this study were to investigate the antimicrobial resistance patterns and biofilm formation in CoNS isolated from cow milk samples submitted to the Onderstepoort Milk Laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 142 confirmed CoNS isolates were used for this study. Biofilm formation was identified in 18% of CoNS tested.<jats:italic>Staphylococcus chromogenes<\/jats:italic>(11%) had the highest proportion of biofilm formation followed by<jats:italic>S. haemolyticus<\/jats:italic>(4.0%),<jats:italic>S. epidermidis<\/jats:italic>,<jats:italic>S. hominis, S. xylosus, and S. simulans<\/jats:italic>with 1% respectively. Ninety percent (90%) of CoNS were resistant to at least one antimicrobial (AMR) and 51% were multidrug-resistant (MDR). Resistance among CoNS was the highest to ampicillin (90%) and penicillin (89%), few isolates resistant to cefoxitin and vancomycin, 9% respectively. Similarly, MDR-<jats:italic>S. haemolyticus<\/jats:italic>(44%), MDR-<jats:italic>S. epidermidis<\/jats:italic>(65%), and MDR-<jats:italic>S<\/jats:italic>.<jats:italic>chromogenes<\/jats:italic>(52%) were mainly resistant to penicillins. The most common resistance patterns observed were resistance to penicillin-ampicillin (16%) and penicillin-ampicillin-erythromycin (10%). Only 42% of biofilm positive CoNS were MDR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The majority of CoNS in this study were resistance to penicillins. In addition, most isolates were \u03b2-lactam resistant and MDR. Biofilm formation among the CoNS in this study was uncommon and there was no significant difference in the proportion of MDR-CoNS based on the ability to form a biofilm.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-019-2175-3","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T17:03:29Z","timestamp":1574787809000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory"],"prefix":"10.1186","volume":"15","author":[{"given":"Lufuno","family":"Phophi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge-Marie","family":"Petzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4609-5151","authenticated-orcid":false,"given":"Daniel Nenene","family":"Qekwana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,26]]},"reference":[{"key":"2175_CR1","doi-asserted-by":"publisher","first-page":"2433","DOI":"10.3168\/jds.S0022-0302(04)73366-4","volume":"87","author":"A Pitk\u00e4l\u00e4","year":"2010","unstructured":"Pitk\u00e4l\u00e4 A, Haveri M, Py\u00f6r\u00e4l\u00e4 S, Myllys V, Honkanen-Buzalski T. 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