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The aim of the study was to determine the frequency of\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                      infection and isolate and identify the genotypes of\n                      <jats:italic>T. gondii<\/jats:italic>\n                      in stray cats in the Mashhad suburb.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and\n                      <jats:italic>T. gondii<\/jats:italic>\n                      -specific PCR. The brain samples were investigated by\n                      <jats:italic>T. gondii<\/jats:italic>\n                      -specific PCR and consequently examined by mice bioassay. The DNA of\n                      <jats:italic>T. gondii<\/jats:italic>\n                      isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the present study,\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      -like oocysts were microscopically observed in 2.2% (4\/175) fecal samples. The presence of\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T\n                      <jats:italic>. gondii<\/jats:italic>\n                      DNA was detected in 4% (7\/175) microscopy-negative samples using PCR.\n                      <jats:italic>T. gondii<\/jats:italic>\n                      was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many\n                      <jats:italic>T. gondii<\/jats:italic>\n                      cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis. The isolated genotyped was type II. Besides, eight\n                      <jats:italic>Toxoplasma-<\/jats:italic>\n                      positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Parasitological and molecular results showed low frequency of\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      infection in the stray cats, and identified the genotype of\n                      <jats:italic>T. gondii<\/jats:italic>\n                      isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-019-2176-2","type":"journal-article","created":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T05:02:43Z","timestamp":1576904563000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran"],"prefix":"10.1186","volume":"15","author":[{"given":"Majid","family":"Khodaverdi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0754-1278","authenticated-orcid":false,"given":"Gholamreza","family":"Razmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,21]]},"reference":[{"key":"2176_CR1","volume-title":"Toxoplasmosis of animals and humans","author":"JP Dubey","year":"2010","unstructured":"Dubey JP. 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Sampling of stray cats was carried out with the permission and cooperation of the Mashhad Municipality and the Veterinary Organization of Khorasan Razavi Province.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"463"}}