{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:19:54Z","timestamp":1790745594076,"version":"4.1.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian National Health and Medical Research Council","award":["1046812"],"award-info":[{"award-number":["1046812"]}]},{"name":"Australian National Health and Medical Research Council","award":["1098337"],"award-info":[{"award-number":["1098337"]}]},{"name":"Australian National Health and Medical Research Council","award":["1131932"],"award-info":[{"award-number":["1131932"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Melioidosis is a tropical infectious disease which is being increasingly recognised throughout the globe. Infection occurs in humans and animals, typically through direct exposure to soil or water containing the environmental bacterium\n                      <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                      . Case clusters of melioidosis have been described in humans following severe weather events and in exotic animals imported into melioidosis endemic zones. Direct transmission of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      between animals and\/or humans has been documented but is considered extremely rare. Between March 2015 and October 2016 eight fatal cases of melioidosis were reported in slender-tailed meerkats (\n                      <jats:italic>Suricata suricatta)<\/jats:italic>\n                      on display at a Wildlife Park in Northern Australia. To further investigate the melioidosis case cluster we sampled the meerkat enclosure and adjacent park areas and performed whole-genome sequencing (WGS) on all culture-positive\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      environmental and clinical isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      WGS confirmed that the fatalities were caused by two different\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      sequence types (STs) but that seven of the meerkat isolates were highly similar on the whole-genome level. Used concurrently with detailed pathology data, our results demonstrate that the seven cases originated from a single original source, but routes of infection varied amongst meerkats belonging to the clonal outbreak cluster. Moreover, in some instances direct transmission may have transpired through wounds inflicted while fighting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Collectively, this study supports the use of high-resolution WGS to enhance epidemiological investigations into transmission modalities and pathogenesis of melioidosis, especially in the instance of a possible clonal outbreak scenario in exotic zoological collections. Such findings from an animal outbreak have important One Health implications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-019-2198-9","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T04:04:20Z","timestamp":1576728260000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Melioidosis fatalities in captive slender-tailed meerkats (Suricata suricatta): combining epidemiology, pathology and whole-genome sequencing supports variable mechanisms of transmission with one health implications"],"prefix":"10.1186","volume":"15","author":[{"given":"Audrey","family":"Rachlin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy","family":"Shilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica R.","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Kaestli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Kleinecke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Rigas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Benedict","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Gurry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart J.","family":"Currie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,19]]},"reference":[{"issue":"11","key":"2198_CR1","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1056\/NEJMra1204699","volume":"367","author":"WJ Wiersinga","year":"2012","unstructured":"Wiersinga WJ, Currie BJ, Peacock SJ. Melioidosis. N Engl J Med. 2012;367(11):1035\u201344.","journal-title":"N Engl J Med"},{"issue":"2","key":"2198_CR2","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","volume":"18","author":"AC Cheng","year":"2005","unstructured":"Cheng AC, Currie BJ. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev. 2005;18(2):383\u2013416.","journal-title":"Clin Microbiol Rev"},{"issue":"11","key":"2198_CR3","doi-asserted-by":"publisher","first-page":"e900","DOI":"10.1371\/journal.pntd.0000900","volume":"4","author":"BJ Currie","year":"2010","unstructured":"Currie BJ, Ward L, Cheng AC. The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study. PLoS Negl Trop Dis. 2010;4(11):e900.","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"2198_CR4","doi-asserted-by":"publisher","first-page":"177","DOI":"10.4269\/ajtmh.2001.65.177","volume":"65","author":"BJ Currie","year":"2001","unstructured":"Currie BJ, Mayo M, Anstey NM, Donohoe P, Haase A, Kemp DJ. A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates. Am J Trop Med Hyg. 2001;65(3):177\u20139.","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"2198_CR5","doi-asserted-by":"publisher","first-page":"e2072","DOI":"10.1371\/journal.pntd.0002072","volume":"7","author":"D Limmathurotsakul","year":"2013","unstructured":"Limmathurotsakul D, Kanoksil M, Wuthiekanun V, Kitphati R, deStavola B, Day NP, Peacock SJ. Activities of daily living associated with acquisition of melioidosis in Northeast Thailand: a matched case-control study. PLoS Negl Trop Dis. 2013;7(2):e2072.","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"2198_CR6","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1038\/nrmicro1385","volume":"4","author":"WJ Wiersinga","year":"2006","unstructured":"Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ. Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei. Nat Rev Microbiol. 2006;4(4):272\u201382.","journal-title":"Nat Rev Microbiol"},{"issue":"3","key":"2198_CR7","doi-asserted-by":"publisher","first-page":"e91682","DOI":"10.1371\/journal.pone.0091682","volume":"9","author":"DS Sarovich","year":"2014","unstructured":"Sarovich DS, Price EP, Webb JR, Ward LM, Voutsinos MY, Tuanyok A, Mayo M, Kaestli M, Currie BJ. Variable virulence factors in Burkholderia pseudomallei (melioidosis) associated with human disease. PLoS One. 2014;9(3):e91682.","journal-title":"PLoS One"},{"issue":"2\u20133","key":"2198_CR8","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0001-706X(99)00065-0","volume":"74","author":"J Low Choy","year":"2000","unstructured":"Low Choy J, Mayo M, Janmaat A, Currie BJ. Animal melioidosis in Australia. Acta Trop. 2000;74(2\u20133):153\u20138.","journal-title":"Acta Trop"},{"issue":"1","key":"2198_CR9","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1055\/s-0034-1398389","volume":"36","author":"BJ Currie","year":"2015","unstructured":"Currie BJ. Melioidosis: evolving concepts in epidemiology, pathogenesis, and treatment. Semin Respir Crit Care Med. 2015;36(1):111\u201325.","journal-title":"Semin Respir Crit Care Med"},{"issue":"7","key":"2198_CR10","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1439-0450.2004.00797.x","volume":"51","author":"LD Sprague","year":"2004","unstructured":"Sprague LD, Neubauer H. Melioidosis in animals: a review on epizootiology, diagnosis and clinical presentation. J Vet Med B Infect Dis Vet Public Health. 2004;51(7):305\u201320.","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"issue":"9","key":"2198_CR11","doi-asserted-by":"publisher","first-page":"1924","DOI":"10.1017\/S0950268816000285","volume":"144","author":"AC Hoger","year":"2016","unstructured":"Hoger AC, Mayo M, Price EP, Theobald V, Harrington G, Machunter B, Choy JL, Currie BJ, Kaestli M. The melioidosis agent Burkholderia pseudomallei and related opportunistic pathogens detected in faecal matter of wildlife and livestock in northern Australia. Epidemiol Infect. 2016;144(9):1924\u201332.","journal-title":"Epidemiol Infect"},{"issue":"2","key":"2198_CR12","doi-asserted-by":"publisher","first-page":"325","DOI":"10.3201\/eid1802.111347","volume":"18","author":"D Limmathurotsakul","year":"2012","unstructured":"Limmathurotsakul D, Thammasart S, Warrasuth N, Thapanagulsak P, Jatapai A, Pengreungrojanachai V, Anun S, Joraka W, Thongkamkoon P, Saiyen P, et al. Melioidosis in animals, Thailand, 2006-2010. Emerg Infect Dis. 2012;18(2):325\u20137.","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"2198_CR13","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3390\/tropicalmed3010031","volume":"3","author":"S Sim","year":"2018","unstructured":"Sim S, Ong C, Gan Y, Wang D, Koh V, Tan Y, Wong M, Chew J, Ling S, Tan B, et al. Melioidosis in Singapore: clinical, veterinary, and environmental perspectives. Trop Med Infect Dis. 2018;3(1):31.","journal-title":"Trop Med Infect Dis"},{"issue":"5","key":"2198_CR14","doi-asserted-by":"publisher","first-page":"E389","DOI":"10.1111\/tbed.12315","volume":"63","author":"T Kasantikul","year":"2016","unstructured":"Kasantikul T, Sommanustweechai A, Polsrila K, Kongkham W, Chaisongkram C, Sanannu S, Kongmakee P, Narongwanichgarn W, Bush M, Sermswan RW, et al. Retrospective study on fatal Melioidosis in captive zoo animals in Thailand. Transbound Emerg Dis. 2016;63(5):E389\u201394.","journal-title":"Transbound Emerg Dis"},{"issue":"6","key":"2198_CR15","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1038\/icb.1950.70","volume":"28","author":"GS Cottew","year":"1950","unstructured":"Cottew GS. Melioidosis in sheep in Queens land; a description of the causal organism. Aust J of Exp Biol Med Sci. 1950;28(6):677\u201383.","journal-title":"Aust J of Exp Biol Med Sci"},{"issue":"6","key":"2198_CR16","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1111\/j.1751-0813.1992.tb07492.x","volume":"69","author":"JC Forbes-Faulkner","year":"1992","unstructured":"Forbes-Faulkner JC, Townsend WL, Thomas AD. Pseudomonas pseudomallei infection in camels. Aust Vet J. 1992;69(6):148.","journal-title":"Aust Vet J"},{"issue":"10","key":"2198_CR17","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1111\/j.1751-0813.2004.tb12607.x","volume":"82","author":"A Janmaat","year":"2004","unstructured":"Janmaat A, Low Choy J, Currie B. Melioidosis in an alpaca (Lama pacos). Aust Vet J. 2004;82(10):622\u201333.","journal-title":"Aust Vet J"},{"issue":"2","key":"2198_CR18","doi-asserted-by":"publisher","first-page":"287","DOI":"10.4269\/ajtmh.14-0115","volume":"91","author":"W Tonpitak","year":"2014","unstructured":"Tonpitak W, Sornklien C, Chawanit M, Pavasutthipaisit S, Wuthiekanun V, Hantrakun V, Amornchai P, Thaipadungpanit J, Day NPJ, Yingst S, et al. Short report: fatal Melioidosis in goats in Bangkok, Thailand. Am J Trop Med Hyg. 2014;91(2):287\u201390.","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"2198_CR19","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1111\/j.1751-0813.1986.tb02953.x","volume":"63","author":"P Ketterer","year":"1986","unstructured":"Ketterer P, Webster W, Shield J, Arthur R, Blackall P, Thomas A. Melioidosis in intensive piggeries in south eastern Queensland. Aust Vet J. 1986;63(5):146\u20139.","journal-title":"Aust Vet J"},{"issue":"24","key":"2198_CR20","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1136\/vr.130.24.525","volume":"130","author":"D Dance","year":"1992","unstructured":"Dance D, King C, Aucken H, Knott C, West P, Pitt T. An outbreak of melioidosis in imported primates in Britain. Vet Rec. 1992;130(24):525\u20139.","journal-title":"Vet Rec"},{"key":"2198_CR21","unstructured":"Kongmakee UM, Noimoon S, Banlunara W. Melioidosis in Captive Slender Tailed Meerkats (Suricata suricatta). In: Proceedings of the 14th Chulalongkorn University Veterinary Conference; 2015 Apr 20-22; Bangkok, Thailand: p. 211\u2013212."},{"key":"2198_CR22","doi-asserted-by":"crossref","unstructured":"Sarovich DS, Garin B, De Smet B, Kaestli M, Mayo M, Vandamme P, Jacobs J, Lompo P, Tahita MC, Tinto H, et al. Phylogenomic Analysis Reveals an Asian Origin for African Burkholderia pseudomallei and Further Supports Melioidosis Endemicity in Africa. mSphere. 2016;1(2).","DOI":"10.1128\/mSphere.00089-15"},{"issue":"11","key":"2198_CR23","doi-asserted-by":"publisher","first-page":"2052","DOI":"10.3201\/eid2111.141802","volume":"21","author":"BJ Currie","year":"2015","unstructured":"Currie BJ, Price EP, Mayo M, Kaestli M, Theobald V, Harrington I, Harrington G, Sarovich DS. Use of whole-genome sequencing to link Burkholderia pseudomallei from air sampling to Mediastinal Melioidosis, Australia. Emerg Infect Dis. 2015;21(11):2052\u20134.","journal-title":"Emerg Infect Dis"},{"key":"2198_CR24","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/1471-2334-7-68","volume":"7","author":"BJ Currie","year":"2007","unstructured":"Currie BJ, Gal D, Mayo M, Ward L, Godoy D, Spratt BG, LiPuma JJ. Using BOX-PCR to exclude a clonal outbreak of melioidosis. BMC Infect Dis. 2007;7:68.","journal-title":"BMC Infect Dis"},{"issue":"1","key":"2198_CR25","doi-asserted-by":"publisher","first-page":"68","DOI":"10.4269\/ajtmh.15-0627","volume":"94","author":"SNJ Chapple","year":"2016","unstructured":"Chapple SNJ, Price EP, Sarovich DS, McRobb E, Mayo M, Kaestli M, Spratt BG, Currie BJ. Burkholderia pseudomallei genotype distribution in the Northern Territory, Australia. Am J Trop Med Hyg. 2016;94(1):68\u201372.","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"2198_CR26","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1128\/JCM.41.5.2068-2079.2003","volume":"41","author":"D Godoy","year":"2003","unstructured":"Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, Spratt BG. Multilocus sequence typing and evolutionary relationships among the causative agents of Melioidosis and Glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol. 2003;41(5):2068\u201379.","journal-title":"J Clin Microbiol"},{"issue":"7","key":"2198_CR27","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.3201\/eid1707.100614","volume":"17","author":"M Mayo","year":"2011","unstructured":"Mayo M, Kaesti M, Harrington G, Cheng AC, Ward L, Karp D, Jolly P, Godoy D, Spratt BG, Currie BJ. Burkholderia pseudomallei in unchlorinated domestic bore water, Tropical Northern Australia. Emerg Infect Dis. 2011;17(7):1283\u20135.","journal-title":"Emerg Infect Dis"},{"issue":"9","key":"2198_CR28","doi-asserted-by":"publisher","first-page":"992","DOI":"10.1099\/jmm.0.000312","volume":"65","author":"EP Price","year":"2016","unstructured":"Price EP, MacHunter B, Spratt BG, Wagner DM, Currie BJ, Sarovich DS. Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species. J Medical Microbiol. 2016;65(9):992\u20137.","journal-title":"J Medical Microbiol"},{"key":"2198_CR29","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1186\/1741-7007-7-78","volume":"7","author":"T Pearson","year":"2009","unstructured":"Pearson T, Giffard P, Beckstrom-Sternberg S, Auerbach R, Hornstra H, Tuanyok A, Price EP, Glass MB, Leadem B, Beckstrom-Sternberg JS, et al. Phylogeographic reconstruction of a bacterial species with high levels of lateral gene transfer. BMC Biol. 2009;7:78.","journal-title":"BMC Biol"},{"issue":"12","key":"2198_CR30","doi-asserted-by":"publisher","first-page":"e1381","DOI":"10.1371\/journal.pntd.0001381","volume":"5","author":"J Dale","year":"2011","unstructured":"Dale J, Price EP, Hornstra H, Busch JD, Mayo M, Godoy D, Wuthiekanun V, Baker A, Foster JT, Wagner DM, et al. Epidemiological tracking and population assignment of the non-clonal bacterium, Burkholderia pseudomallei. PLoS Negl Trop Dis. 2011;5(12):e1381.","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"2198_CR31","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1128\/AEM.03013-15","volume":"82","author":"EP Price","year":"2016","unstructured":"Price EP, Sarovich DS, Smith EJ, MacHunter B, Harrington G, Theobald V, Hall CM, Hornstra HM, McRobb E, Podin Y, et al. Unprecedented Melioidosis cases in northern Australia caused by an Asian Burkholderia pseudomallei strain identified by using large-scale comparative genomics. Appl Environ Microbiol. 2016;82(3):954\u201363.","journal-title":"Appl Environ Microbiol"},{"issue":"11","key":"2198_CR32","first-page":"e000139","volume":"3","author":"A Aziz","year":"2017","unstructured":"Aziz A, Sarovich DS, Harris TM, Kaestli M, McRobb E, Mayo M, Currie BJ, Price EP. Suspected cases of intracontinental Burkholderia pseudomallei sequence type homoplasy resolved using whole-genome sequencing. Microb Genom. 2017;3(11):e000139.","journal-title":"Microb Genom"},{"issue":"4","key":"2198_CR33","doi-asserted-by":"publisher","first-page":"1144","DOI":"10.1128\/JCM.03453-14","volume":"53","author":"E McRobb","year":"2015","unstructured":"McRobb E, Sarovich DS, Price EP, Kaestli M, Mayo M, Keim P, Currie BJ. Tracing Melioidosis Back to the source: using whole-genome sequencing to investigate an outbreak originating from a contaminated domestic water supply. J Clin Microbiol. 2015;53(4):1144.","journal-title":"J Clin Microbiol"},{"issue":"1","key":"2198_CR34","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1111\/j.1365-2958.2004.04528.x","volume":"56","author":"MP Stevens","year":"2005","unstructured":"Stevens MP, Stevens JM, Jeng RL, Taylor LA, Wood MW, Hawes P, Monaghan P, Welch MD, Galyov EE. Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei. Mol Microbiol. 2005;56(1):40\u201353.","journal-title":"Mol Microbiol"},{"issue":"1","key":"2198_CR35","first-page":"69","volume":"63","author":"A Dodin","year":"1986","unstructured":"Dodin A, Galimand M. origin, course and recession of an infectious disease, melioidosis, in temperate countries. Arch Inst Pasteur Tunis. 1986;63(1):69\u201373.","journal-title":"Arch Inst Pasteur Tunis"},{"issue":"12","key":"2198_CR36","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1097\/01.inf.0000145548.79395.da","volume":"23","author":"A Ralph","year":"2004","unstructured":"Ralph A, McBride J, Currie BJ. Transmission of Burkholderia pseudomallei via breast milk in northern Australia. Pediatr Infect Dis J. 2004;23(12):1169\u201371.","journal-title":"Pediatr Infect Dis J"},{"issue":"9","key":"2198_CR37","doi-asserted-by":"publisher","first-page":"3418","DOI":"10.1128\/JCM.01219-14","volume":"52","author":"D Limmathurotsakul","year":"2014","unstructured":"Limmathurotsakul D, Holden MTG, Coupland P, Price EP, Chantratita N, Wuthiekanun V, Amornchai P, Parkhill J, Peacock SJ. Microevolution of Burkholderia pseudomallei during an acute infection. J Clin Microbiol. 2014;52(9):3418.","journal-title":"J Clin Microbiol"},{"issue":"1","key":"2198_CR38","doi-asserted-by":"publisher","first-page":"e1000725","DOI":"10.1371\/journal.ppat.1000725","volume":"6","author":"E Price","year":"2010","unstructured":"Price E, Hornstra HM, Limmathurotsakul D, Max TL, Sarovich D, Vogler AJ, Dale JL, Ginther JL, Leadem B, Colman RE, et al. Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis. PLoS Path. 2010;6(1):e1000725.","journal-title":"PLoS Path"},{"issue":"8","key":"2198_CR39","first-page":"e000117","volume":"3","author":"DS Sarovich","year":"2017","unstructured":"Sarovich DS, Chapple SNJ, Price EP, Mayo M, Holden MTG, Peacock SJ, Currie BJ. Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island. Microb Genom. 2017;3(8):e000117.","journal-title":"Microb Genom"},{"issue":"3","key":"2198_CR40","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1111\/j.1751-0813.2000.tb10593.x","volume":"78","author":"C Hicks","year":"2000","unstructured":"Hicks C, Kinoshita R, Ladds P. Pathology of melioidosis in captive marine mammals. Aust Vet J. 2000;78(3):193\u20135.","journal-title":"Aust Vet J"},{"key":"2198_CR41","unstructured":"Zachary JF, McGavin MD. In: Pathologic basis of veterinary disease-E-book: 5th edition. St. Louis: Elsevier Health Sciences; 2013. p.120\u2013127."},{"issue":"6","key":"2198_CR42","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1111\/j.1751-0813.1970.tb15788.x","volume":"46","author":"RJ Rogers","year":"1970","unstructured":"Rogers RJ, Andersen DJ. Intra-uterine infection of a pig by Pseudomonas pseudomallei. Aust Vet J. 1970;46(6):292.","journal-title":"Aust Vet J"},{"key":"2198_CR43","first-page":"405","volume":"5","author":"J Cullen","year":"2012","unstructured":"Cullen J, Brown DL. Hepatobiliary system and exocrine pancreas. Pathol Basis Vet Dis. 2012;5:405\u201357.","journal-title":"Pathol Basis Vet Dis"},{"key":"2198_CR44","doi-asserted-by":"crossref","unstructured":"Lopez A, Martinson SA. Respiratory system, mediastinum and pleurae. Pathol Basis Vet Dis. 2017:471\u2013560.","DOI":"10.1016\/B978-0-323-35775-3.00009-6"},{"key":"2198_CR45","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/S0368-1742(63)80038-7","volume":"73","author":"A Omar","year":"1963","unstructured":"Omar A. Pathology of melioidosis in pigs, goats and a horse. J Comp Pathol Ther. 1963;73:359\u2013IN334.","journal-title":"J Comp Pathol Ther"},{"key":"2198_CR46","volume-title":"Melioidosis: a century of observation and research","author":"N Ketheesan","year":"2012","unstructured":"Ketheesan N. Melioidosis: a century of observation and research. Amsterdam: Elsevier; 2012."},{"issue":"3","key":"2198_CR47","doi-asserted-by":"publisher","first-page":"e2105","DOI":"10.1371\/journal.pntd.0002105","volume":"7","author":"D Limmathurotsakul","year":"2013","unstructured":"Limmathurotsakul D, Dance DAB, Wuthiekanun V, Kaestli M, Mayo M, Warner J, Wagner DM, Tuanyok A, Wertheim H, Yoke Cheng T, et al. Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei. PLoS Negl Trop Dis. 2013;7(3):e2105.","journal-title":"PLoS Negl Trop Dis"},{"issue":"8","key":"2198_CR48","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1016\/0923-2508(92)90107-Y","volume":"143","author":"X de Lamballerie","year":"1992","unstructured":"de Lamballerie X, Zandotti C, Vignoli C, Bollet C, de Micco P. A one-step microbial DNA extraction method using \u201cChelex 100\u201d suitable for gene amplification. Res Microbiol. 1992;143(8):785\u201390.","journal-title":"Res Microbiol"},{"issue":"1","key":"2198_CR49","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1128\/JCM.44.1.85-90.2006","volume":"44","author":"RT Novak","year":"2006","unstructured":"Novak RT, Glass MB, Gee JE, Gal D, Mayo MJ, Currie BJ, Wilkins PP. Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei. J Clin Microbiol. 2006;44(1):85\u201390.","journal-title":"J Clin Microbiol"},{"key":"2198_CR50","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1186\/1756-0500-7-618","volume":"7","author":"DS Sarovich","year":"2014","unstructured":"Sarovich DS, Price EP. SPANDx: a genomics pipeline for comparative analysis of large haploid whole genome re-sequencing datasets. BMC Res. 2014;7:618.","journal-title":"BMC Res"},{"key":"2198_CR51","doi-asserted-by":"crossref","unstructured":"Wilgenbusch JC, Swofford D: Inferring evolutionary trees with PAUP*. Curr Protoc Bioinformatics 2003, Chapter 6:Unit 6 4.","DOI":"10.1002\/0471250953.bi0604s00"},{"issue":"W1","key":"2198_CR52","doi-asserted-by":"publisher","first-page":"W242","DOI":"10.1093\/nar\/gkw290","volume":"44","author":"I Letunic","year":"2016","unstructured":"Letunic I, Bork P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res. 2016;44(W1):W242\u20135.","journal-title":"Nucleic Acids Res"},{"key":"2198_CR53","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1186\/1471-2105-11-595","volume":"11","author":"KA Jolley","year":"2010","unstructured":"Jolley KA, Maiden MCJ. BIGSdb: scalable analysis of bacterial genome variation at the population level. BMC Bioinform. 2010;11:595.","journal-title":"BMC Bioinform"},{"issue":"4","key":"2198_CR54","doi-asserted-by":"publisher","first-page":"ofz091","DOI":"10.1093\/ofid\/ofz091","volume":"6","author":"JR Webb","year":"2019","unstructured":"Webb JR, Sarovich DS, Price E, Ward LM, Mayo M, Currie BJ. Burkholderia pseudomallei lipopolysaccharide genotype does not correlate with severity or outcome in melioidosis: host risk factors remain the critical determinant. Open Forum Infect Dis. 2019;6(4):ofz091.","journal-title":"Open Forum Infect Dis"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-019-2198-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12917-019-2198-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-019-2198-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T18:02:52Z","timestamp":1665252172000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-019-2198-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["2198"],"URL":"https:\/\/doi.org\/10.1186\/s12917-019-2198-9","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"23 May 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 November 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 December 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that there are no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"458"}}