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CGR has also many species of ticks and mosquitos that serve as competent vectors for important animal and human pathogens. In this study, we tested dogs from six different animal shelters in the CGR for Rocky Mountain spotted fever (RMSF), anaplasmosis, Lyme disease, canine ehrlichiosis and canine heartworm disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sera from 157 shelter dogs were tested for antibodies to RMSF agent,<jats:italic>Rickettsia rickettsii<\/jats:italic>, using an indirect immunofluorescence assay. Sixty-six dogs (42.0%) were positive for either IgM or IgG, or both IgM and IgG antibodies to<jats:italic>R. rickettsii<\/jats:italic>. Moreover, the same set of sera (<jats:italic>n<\/jats:italic>\u2009=\u2009157) plus an and additional sera (<jats:italic>n<\/jats:italic>\u2009=\u200975) from resident dogs at the same shelters were tested using the SNAP 4Dx Plus. Of 232 dogs tested, two (0.9%) were positive for antibodies to<jats:italic>Anaplasma phagocytophilum\/A. platys<\/jats:italic>, nine (3.9%) were positive for antibodies to<jats:italic>Borrelia burgdorferi<\/jats:italic>, 23 (9.9%) for positive for antibodies to<jats:italic>Ehrlichia canis\/E. ewingii<\/jats:italic>, and 13 (5.6%) were positive for<jats:italic>Dirofilaria immitis<\/jats:italic>antigen. Co-infection with two or more etiologic agents was detected in five animals. Three dogs had antibodies to both<jats:italic>B. burgdorferi<\/jats:italic>and<jats:italic>E. canis\/E. ewingii<\/jats:italic>, and two dogs were positive for<jats:italic>D. immitis<\/jats:italic>antigen and antibodies to<jats:italic>B. burgdorferi<\/jats:italic>and<jats:italic>E. canis\/E. ewingii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Shelter dogs in the CGR are exposed to a number of important vector-borne pathogens. Further studies are required to ascertain the roles these animals play in maintenance and transmission of these pathogens.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-020-02440-1","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T07:02:49Z","timestamp":1593500569000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Serosurvey of arthropod-borne diseases among shelter dogs in the Cumberland Gap Region of the United States"],"prefix":"10.1186","volume":"16","author":[{"given":"Gilbert","family":"Patterson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Tanhauser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn","family":"Spangler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Faulkner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vina","family":"Faulkner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Kish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Gruszynski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemant","family":"Naikare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele D.","family":"Coarsey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5506-5763","authenticated-orcid":false,"given":"Ashutosh","family":"Verma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,6,30]]},"reference":[{"key":"2440_CR1","unstructured":"American Heartworm Society. 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All sample collections were handled in strict accordance with U.S. Animal Welfare Act, its amendments and associated Regulations (https:\/\/www.nal.usda.gov\/awic\/animal-welfare-act). Memoranda of Understanding (MOUs) established between all parties provided informed consent for collection of blood samples and analysis of clinical disease.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"221"}}