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Several transmission routes, including those related to reproduction, have been described. Indeed, the pathogen can colonize the female reproductive tract after artificial insemination (AI) with contaminated semen. <jats:italic>Lactobacillus<\/jats:italic> spp.-based probiotics have been used for vaginal dysbiosis treatment in women and cows although their role in controlling cervico-vaginal infections due to <jats:italic>M. bovis<\/jats:italic> is unknown. The objective of the present work is to assess the viability of <jats:italic>M. bovis<\/jats:italic> (PG45, NCTC 10131) in experimentally contaminated cervical mucus after the addition of <jats:italic>Lactobacillus<\/jats:italic> spp. at different concentrations as a competing agent and pH acidifier.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The addition of probiotic at a concentration higher than 10<jats:sup>8<\/jats:sup> colony forming units (CFU\/mL had a detrimental effect (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) on mycoplasma viability in cervical mucus. This coincided with a significant LAB growth and an important decrease in pH from 8.4 to 5.6 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). However, after the addition of less concentrated probiotic, <jats:italic>M. bovis<\/jats:italic> survival was not affected and there was no significant LAB growth despite the drop of pH from 8.4 to 6.73 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The addition of concentrations higher than 10<jats:sup>8<\/jats:sup>\u2009CFU\/mL of <jats:italic>Lactobacillus<\/jats:italic> spp. negatively affects <jats:italic>M. bovis<\/jats:italic> viability in bovine cervical mucus under in vitro conditions. Although the effect observed on the pathogen viability seems to be related to the pH decrease after LAB proliferation in cervical mucus, further studies are necessary to elucidate if other factors are implicated. Nevertheless, the administration of <jats:italic>Lactobacillus<\/jats:italic> spp.-based probiotics might be used in the future to control <jats:italic>M. bovis<\/jats:italic> proliferation in the cervico-vaginal tract of cows.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12917-020-02454-9","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T11:03:32Z","timestamp":1595243012000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus"],"prefix":"10.1186","volume":"16","author":[{"given":"A.","family":"Garc\u00eda-Gal\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"De la Fe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Gomis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Bataller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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Verbal informed consent was obtained from the owner of the animals and this procedure was approved by the committee of the General Directorate of Agriculture, Livestock and, Fisheries of Valencian Community (2017\/VSC\/PEA\/00245) and the University of Murcia committee (REGA ES300305440012).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"251"}}