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In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3.<\/jats:p>\n                    <jats:p>Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Positive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-020-02489-y","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T13:03:48Z","timestamp":1597064628000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3456-7438","authenticated-orcid":false,"given":"Jan","family":"Plut","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urska","family":"Jamnikar-Ciglenecki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irena","family":"Golinar-Oven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Knific","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Stukelj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"2489_CR1","doi-asserted-by":"publisher","unstructured":"Phan TG, Giannitti F, Rossow S, Marthaler D, Knutson TP, Li L, et al. 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In accordance with Directive 2010\/63\/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes and Slovenian Animal Protection Law (Uradni list RS \u0161t. 38\/2013 and 21\/2018), non-experimental clinical veterinary practices and practices not likely to cause pain, suffering distress or lasting harm equivalent to, or higher than that caused by the introduction of a needle, is not considered as an experiment on animals and any consent by national ethics committee is deemed unnecessary. Verbal and written consents for participation in the study were obtained from farm owners\/farm managers; this is stated in the document Resolution: 5-5-2020\/3 issued by Committee for Animal Welfare of Veterinary faculty.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"281"}}