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Results of nested PCR assay targeting 18S rRNA gene and partial gene sequencing revealed that seven animals were infected with <jats:italic>Babesia<\/jats:italic> species, five dogs (5\/115, 4.3%) and two cats (2\/25, 8.0%). Sequence analysis showed that four dogs (3.5%) were positive for <jats:italic>Babesia canis<\/jats:italic>, and the other one for <jats:italic>Babesia vogeli<\/jats:italic> (0.87%). The two cats were infected by <jats:italic>Babesia hongkongensis.<\/jats:italic><\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The findings of this study will expand knowledge of the distribution of <jats:italic>Babesia<\/jats:italic> species and provide important epidemiological information for the control of animal babesiosis in China.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12917-020-02500-6","type":"journal-article","created":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T17:02:34Z","timestamp":1597683754000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular evidence of piroplasm infection in companion animals in Hunan Province, China"],"prefix":"10.1186","volume":"16","author":[{"given":"Jinming","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyun","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiquan","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,17]]},"reference":[{"issue":"1","key":"2500_CR1","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1111\/j.1476-4431.2009.00493.x","volume":"20","author":"AL Ayoob","year":"2010","unstructured":"Ayoob AL, Prittie J, Hackner SG. 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All the procedures were conducted according to the Animal Ethics Procedures and Guidelines of the People\u2019s Republic of China.Each of the pets\u2019 owners wrote consent and consented to this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"297"}}