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Every year, <jats:italic>Salmonella<\/jats:italic> infections pose a serious threat to the poultry industry in developing countries, especially China. In this study, a total of 84 <jats:italic>Salmonella<\/jats:italic> isolates recovered from sick and healthy-looking chickens in central China were characterized by serotyping, MLST-based strain typing, presence of potential virulence factors, and antimicrobial resistance profiles.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Result<\/jats:title>\n<jats:p>Data showed that the main serotypes of <jats:italic>Salmonella<\/jats:italic> isolates in central China were <jats:italic>Salmonella enterica<\/jats:italic> serovar Gallinarum biovar Pullorum<jats:italic>, Salmonella enterica<\/jats:italic> serovar Gallinarum biovar Gallinarum, <jats:italic>Salmonella enterica<\/jats:italic> serovar Enteritidis and <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium, and among them, <jats:italic>S<\/jats:italic>. Pullorum was the dominant type in both sick and healthy-looking chickens, accounting for 43.9 and 46.5%, respectively, while <jats:italic>S<\/jats:italic>. Enteritidis was only found in healthy-looking chickens. All isolates exhibited higher resistance rates to ampicillin (97.6%), tetracycline (58.3%) and colistin (51.2%), and among these isolates, 49.5% were resistant to more than three drugs in different combinations. <jats:italic>S<\/jats:italic>. Enteritidis was the most severe multidrug-resistant serotype, which showed higher resistance rates to colistin, meropenem and ciprofloxacin. Multilocus sequence typing (MLST) revealed that <jats:italic>S<\/jats:italic>. Gallinarum and <jats:italic>S<\/jats:italic>. Enteritidis isolates were clustered in clade 1, which belonged to two and one STs, respectively. All <jats:italic>S<\/jats:italic>. Typhimurium isolates were clustered in clade 3, and belonged to three STs. However, <jats:italic>S<\/jats:italic>. Pullorum were distributed in three clades, which belonged to 7 STs. Twenty-seven virulence-associated genes were detected, and expected <jats:italic>cdtB<\/jats:italic>, which was absent in all the isolates, the other 26 genes were conserved in the closely related <jats:italic>Salmonella<\/jats:italic> serogroup D (<jats:italic>S<\/jats:italic>. Enteritidis, <jats:italic>S<\/jats:italic>. Pullorum, and <jats:italic>S<\/jats:italic>. Gallinarum).<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p><jats:italic>Salmonella<\/jats:italic> serogroup D was the major subgroup, and <jats:italic>S<\/jats:italic>. Pullorum was the most common type in sick and healthy-looking chickens in central China. Drug resistance assays showed serious multiple antimicrobial resistances, and <jats:italic>S<\/jats:italic>. Enteritidis was the most severe drug-resistant serotype. MLST showed that there was correlation between serotypes and genotypes in most <jats:italic>Salmonella<\/jats:italic> isolates, except <jats:italic>S<\/jats:italic>. Pullorum, which showed complicated genetic diversity firstly. These results provide important epidemiological information for us to control <jats:italic>Salmonella<\/jats:italic> in chickens.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12917-020-02513-1","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T12:02:32Z","timestamp":1597924952000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Characterization of Salmonella spp. isolated from chickens in Central China"],"prefix":"10.1186","volume":"16","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiluo","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyuan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingping","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huabin","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zishu","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"2513_CR1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.vetmic.2018.07.013","volume":"223","author":"Q Li","year":"2018","unstructured":"Li Q, Wang X, Xia J, Yuan Y, Yin C, Xu L, Li Y, Jiao X. 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The experiments were approved by the Ethics Committee of Hubei Academy of Agricultural Sciences according to Hubei Province Laboratory Animal Management Regulations \u2013 2005. The animals used in this study were derived from commercial sources, and the owners\u2019 consent was not required.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"299"}}