{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:22:48Z","timestamp":1792790568358,"version":"4.5.0"},"reference-count":12,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T00:00:00Z","timestamp":1606953600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"publisher","award":["2017YFD0501200"],"award-info":[{"award-number":["2017YFD0501200"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p><jats:italic>Fasciola hepatica<\/jats:italic> is an important zoonotic parasite that causes fasciolosis in a broad range of animals. No information is available about the prevalence of <jats:italic>F. hepatica<\/jats:italic> in P\u00e8re David\u2019s deer (<jats:italic>Elaphurus davidianus<\/jats:italic>), an endangered species in the world. Therefore, the purpose of the study was to evaluate the prevalence of fasciolosis in P\u00e8re David\u2019s deer in the Dafeng Elk National Natural Reserve, Jiangsu province, China.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>In this study, 142 fecal samples from P\u00e8re David\u2019s deer were analyzed for <jats:italic>F. hepatica<\/jats:italic> by microscopy and nest-PCR. Only one sample was positive for <jats:italic>F. hepatica<\/jats:italic> according to microscopy examination, while 18 of 142 (12.68, 95%CI: 2.841\u201322.45%) samples were positive for <jats:italic>F. hepatica<\/jats:italic> according to nest-PCR results.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This is the first report of prevalence of <jats:italic>F. hepatica<\/jats:italic> in P\u00e8re David\u2019s deer. The prevalence data indicated that <jats:italic>F. hepatica<\/jats:italic> was also present in this endangered animal, which may cause a potential threat to this precious species.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12917-020-02694-9","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T11:03:41Z","timestamp":1606993421000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["First report on the prevalence of Fasciola hepatica in the endangered P\u00e8re David\u2019s deer (Elaphurus davidianus) in China"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1452-1299","authenticated-orcid":false,"given":"Si-Yang","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Zhi","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Min","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Min","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,3]]},"reference":[{"key":"2694_CR1","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/B978-0-12-800182-0.00002-7","volume":"85","author":"CA Alvarez Rojas","year":"2014","unstructured":"Alvarez Rojas CA, Jex AR, Gasser RB, Scheerlinck JP. Techniques for the diagnosis of Fasciola infections in animals: room for improvement. Adv Parasitol. 2014;85:65\u2013107.","journal-title":"Adv Parasitol"},{"issue":"6","key":"2694_CR2","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1016\/j.pt.2016.03.002","volume":"32","author":"JM Kelley","year":"2016","unstructured":"Kelley JM, Elliott TP, Beddoe T, Anderson G, Skuce P, Spithill TW. Current threat of Triclabendazole resistance in Fasciola hepatica. Trends Parasitol. 2016;32(6):458\u201369.","journal-title":"Trends Parasitol"},{"key":"2694_CR3","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.ijppaw.2018.12.010","volume":"8","author":"JL Zhang","year":"2019","unstructured":"Zhang JL, Si HF, Zhou XZ, Shang XF, Li B, Zhang JY. High prevalence of fasciolosis and evaluation of the efficacy of anthelmintics against Fasciola hepatica in buffaloes in Guangxi, China. 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All P\u00e8re David\u2019s deer were handled in accordance with good animal practices required by the Animal Ethics Procedures and Guidelines of the People\u2019s Republic of China.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"473"}}