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Recently, various recombinant proteins have been used in serological tests for diagnosing toxoplasmosis. The production of these antigens is associated with live tachyzoites obtained from cell cultures or laboratory animals for genomic extraction to amplify target genes. Synthetic genes have gained a key role in recombinant protein production. For the first time, we demonstrated the production of the recombinant protein of the\n                      <jats:italic>T. gondii<\/jats:italic>\n                      dense granular antigen 8 (TgGRA8) gene based on commercial gene synthesis. Recombinant TgGRA8 plasmids were successfully expressed in an\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      system. The recombinant protein was affinity-purified and characterized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Furthermore, the diagnostic potential of the recombinant protein was assessed using 306 field serum samples from goats via indirect enzyme-linked immunosorbent assay (iELISA) and the latex agglutination test (LAT).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Western blotting using known positive serum samples from goats identified a single antigen at the expected molecular weight of TgGRA8 (27\u2009kDa). iELISA illustrated that 15.40% of goat samples were positive for\n                      <jats:italic>T. gondii<\/jats:italic>\n                      -specific IgG antibodies. In addition, TgGRA8 provided high sensitivity and specificity, with significant concordance (91.83) and kappa values (0.69) compared with the results obtained using LAT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings highlight the production of a recombinant protein from a synthetic TgGRA8 gene and the ability to detect\n                      <jats:italic>T. gondii<\/jats:italic>\n                      infection in field samples. The sensitivity and specificity of TgGRA8 demonstrated that this protein could be a good serological marker for detecting specific IgG in goat sera.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-020-02719-3","type":"journal-article","created":{"date-parts":[[2021,1,9]],"date-time":"2021-01-09T05:06:49Z","timestamp":1610168809000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goats"],"prefix":"10.1186","volume":"17","author":[{"given":"Charoonluk","family":"Jirapattharasate","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruenruetai","family":"Udonsom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apichai","family":"Prachasuphap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kodcharad","family":"Jongpitisub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panadda","family":"Dhepakson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,9]]},"reference":[{"key":"2719_CR1","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","volume":"363","author":"JG Montoya","year":"2004","unstructured":"Montoya JG, Liesenfeld O. 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MUVS-2018-03-09). The animals were handled humanely in strict accordance with the requirements of the Animal Ethics Procedures and Guidelines of Institute of Animals for Scientific purpose Development (IAD), Thailand. Before signing a consent, the owners of the selected farms were informed of the study and provided their approval for sampling of the goats. All samples collection process was conducted by local authorities and veterinarians.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"27"}}