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The aim of the present study was to study the antibiotic susceptibility in isolates of <jats:italic>Staphylococcus<\/jats:italic> spp. and <jats:italic>Escherichia<\/jats:italic> (<jats:italic>E<\/jats:italic>.) <jats:italic>coli<\/jats:italic> obtained from healthy pigs originating from nineteen herds enrolled in a study on herd health management in Lira district, northern Uganda. Skin and nasal swabs were analyzed for the presence of <jats:italic>Staphylococcus<\/jats:italic> spp., and selectively cultivated to investigate the presence of methicillin-resistant <jats:italic>Staphylococcus<\/jats:italic> (<jats:italic>S<\/jats:italic>.) <jats:italic>aureus<\/jats:italic> (MRSA), and rectal swabs were analyzed for the presence of <jats:italic>E. coli<\/jats:italic>. Antibiotic susceptibility was tested by broth micro-dilution. Information on the antibiotic usage and treatment regimens during the previous year was gathered using structured interviews and longitudinal data.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In <jats:italic>Staphylococcus<\/jats:italic> spp., resistance to penicillin (10\/19 isolates; 53%), fusidic acid (42%) and tetracycline (37%) were most commonly found. In <jats:italic>E. coli<\/jats:italic>, resistance to sulfamethoxazole (46\/52 isolates; 88%), tetracycline (54%) and trimethoprim (17%) was most frequent. Methicillin-resistant <jats:italic>S. aureus<\/jats:italic> was found in one sample (1\/50; 2%). Multi-drug resistant isolates of <jats:italic>Staphylococcus<\/jats:italic> spp. and <jats:italic>E. coli<\/jats:italic> were found in 54 and 47% of the herds, respectively. At the herd level, no associations could be made between antibiotic resistance and herd size or treatment regimens for either of the bacteria.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In conclusion, resistance to important antibiotics frequently used in animals in Uganda was common, and the presence of MRSA was demonstrated, in Ugandan pig herds.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-020-02727-3","type":"journal-article","created":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T18:02:40Z","timestamp":1610992960000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The presence of antibiotic-resistant Staphylococcus spp. and Escherichia coli in smallholder pig farms in Uganda"],"prefix":"10.1186","volume":"17","author":[{"given":"K.","family":"Ikwap","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4227-4830","authenticated-orcid":false,"given":"E.","family":"Gertzell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Hansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Dahlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Selling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Magnusson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Dione","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jacobson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"issue":"2","key":"2727_CR1","doi-asserted-by":"publisher","first-page":"96","DOI":"10.3109\/03009734.2014.901445","volume":"119","author":"B Bengtsson","year":"2014","unstructured":"Bengtsson B, Greko C. 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Written and oral informed consent was obtained from the farmers before the beginning of the study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"31"}}