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Hard ticks are obligate haematophagous ectoparasites that serve as vectors of <jats:italic>Borrelia burgdorferi<\/jats:italic>. Studies on the presence of Lyme borreliosis in Egyptian animals and associated ticks are scarce.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>This study was conducted to detect <jats:italic>B. burgdorferi<\/jats:italic> in different tick vectors and animal hosts. Three hundred animals (dogs=100, cattle=100, and camels=100) were inspected for tick infestation. Blood samples from 160 tick-infested animals and their associated ticks (<jats:italic>n<\/jats:italic>=1025) were collected and examined for the infection with <jats:italic>B. burgdorferi<\/jats:italic> by polymerase chain reaction (PCR) and sequencing of the <jats:italic>16S rRNA<\/jats:italic> gene. The identified tick species were characterized molecularly by PCR and sequencing of the ITS2 region.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The overall tick infestation rate among examined animals was 78.33% (235\/300). The rate of infestation was significantly higher in camels (90%), followed by cattle (76%) and dogs (69%); (<jats:italic>P<\/jats:italic> = 0.001). <jats:italic>Rhipicephalus sanguineus<\/jats:italic>, <jats:italic>Rhipicephalus (Boophilus) annulatus<\/jats:italic>, and both <jats:italic>Hyalomma dromedarii<\/jats:italic> and <jats:italic>Amblyomma variegatum,<\/jats:italic> were morphologically identified from infested dogs, cattle, and camels; respectively. Molecular characterization of ticks using the ITS2 region confirmed the morphological identification, as well as displayed high similarities of <jats:italic>R. sanguineus, H. dromedarii,<\/jats:italic> and <jats:italic>A. Variegatu<\/jats:italic> with ticks identified in Egypt and various continents worldwide. Just one dog (1.67%) and its associated tick pool of <jats:italic>R. sanguineus<\/jats:italic> were positive for <jats:italic>B. burgdorferi<\/jats:italic> infection. The <jats:italic>16S rRNA<\/jats:italic> gene sequence for <jats:italic>B. burgdorferi<\/jats:italic> in dog and <jats:italic>R. sanguineus<\/jats:italic> tick pool showed a 100% homology.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Analyzed data revealed a relatively low rate of <jats:italic>B. burgdorferi<\/jats:italic> infection, but a significantly high prevalence of tick infestation among domesticated animals in Egypt, which possesses a potential animal and public health risk. Additionally, molecular characterization of ticks using the ITS2 region was a reliable tool to discriminate species of ticks and confirmed the morphological identification.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-020-02733-5","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T11:06:46Z","timestamp":1611572806000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Evidence of the presence of Borrelia burgdorferi in dogs and associated ticks in Egypt"],"prefix":"10.1186","volume":"17","author":[{"given":"R.","family":"Elhelw","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Elhariri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Abuowarda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Ismael","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Farag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,25]]},"reference":[{"key":"2733_CR1","first-page":"192","volume":"8","author":"NM Pukhovskaya","year":"2019","unstructured":"Pukhovskaya NM, Morozova OV, Vysochina NP, Belozerova NB, Ivanov LI. 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(Institutional Animal Care and Use Committee), Vet CU. IACUC (VetCU1022019070).We had been given oral consent from the owners of the animals as we had just taken ticks and blood samples.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"49"}}