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Many<jats:italic>Enterobacteriaceae\u00a0<\/jats:italic>cannot be treated anymore with third generation cephalosporins (3GC) due to\u00a0the production of certain 3GC hydrolysing enzymes (extended spectrum beta-lactamases, ESBLs). The role of animals as carriers and vectors of multi-resistant bacteria in different geographical regions is poorly understood. Therefore, we investigated the occurrence and molecular characteristics of ESBL-producing<jats:italic>Escherichia coli<\/jats:italic>(<jats:italic>E. coli<\/jats:italic>) in wild birds and slaughtered cattle in Ibadan, Nigeria.<\/jats:p><jats:p>Cattle faecal samples (<jats:italic>n<\/jats:italic>= 250) and wild bird pooled faecal samples (cattle egrets,<jats:italic>Bubulcus ibis<\/jats:italic>,<jats:italic>n<\/jats:italic>= 28; white-faced whistling duck,<jats:italic>Dendrocygna viduata, n<\/jats:italic>= 24) were collected and cultured on cefotaxime-eosin methylene blue agar. Antimicrobial susceptibility was determined by agar diffusion assays and all\u00a03GC resistant isolates were genotypically characterised for\u00a0AMR genes, virulence associated genes (VAGs) and serotypes using DNA microarray-based assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 3GC resistant isolates were<jats:italic>E. coli<\/jats:italic>: cattle (<jats:italic>n<\/jats:italic>= 53), egrets (<jats:italic>n<\/jats:italic>= 87) and whistling duck (<jats:italic>n<\/jats:italic>= 4); cultured from 32\/250 (12.8%), 26\/28 (92.9%), 2\/24(8.3%), cattle, egrets and whistling duck faecal samples, respectively.<jats:italic>bla<\/jats:italic>CTX-M gene family was prevalent;<jats:italic>bla<\/jats:italic>CTX-M15 (83.3%) predominated over<jats:italic>bla<\/jats:italic>CTX-M9 (11.8%). All were susceptible to carbapenems. The majority of isolates were resistant to at least one of the other tested antimicrobials; multidrug resistance was highest in the isolates recovered from egrets.<\/jats:p><jats:p>The isolates harboured diverse repositories of other AMR genes (including<jats:italic>strB<\/jats:italic>and\u00a0<jats:italic>sul2<\/jats:italic>), integrons (predominantly class 1) and VAGs. The isolates recovered from egrets harboured more AMR genes; eight were unique to these isolates including<jats:italic>tetG<\/jats:italic>,<jats:italic>gepA<\/jats:italic>, and<jats:italic>floR<\/jats:italic>. The prevalent VAGs included<jats:italic>hemL<\/jats:italic>and<jats:italic>iss<\/jats:italic>; while 14 (including<jats:italic>sepA<\/jats:italic>) were unique to certain animal isolates<jats:italic>. E. coli<\/jats:italic>serotypes O9:H9, O9:H30 and O9:H4 predominated. An identical phenotypic microarray profile was detected in three isolates from egrets and cattle, indicative of a clonal relationship amongst these isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Wild birds and cattle harbour diverse ESBL-producing<jats:italic>E. coli<\/jats:italic>populations with potential of inter-species dissemination and virulence. Recommended guidelines to balance public health and habitat conservation should be implemented with continuous surveillance.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-020-02734-4","type":"journal-article","created":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T18:02:40Z","timestamp":1610992960000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Molecular characterisation of extended-spectrum \u00df-lactamase producing Escherichia coli in wild birds and cattle, Ibadan, Nigeria"],"prefix":"10.1186","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8580-8591","authenticated-orcid":false,"given":"Kayode","family":"Fashae","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Engelmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Monecke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha D.","family":"Braun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Ehricht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"key":"#cr-split#-2734_CR1.1","doi-asserted-by":"crossref","unstructured":"Coque TM, Baquero F, Canton R. 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Clearance by an ethics committee was not necessary. The specimens were obtained by non-invasive procedures as described in the materials and methods (i.e., rectal swabbing of cattle at abattoir and collection of bird faecal droppings from the environment without animal contact). No animal experiments were carried out for this study. Verbal informed consent of personnels, cattle owners and butchers (at the abattoir) was obtained to collect the faecal samples.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"33"}}