{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:21:22Z","timestamp":1772166082134,"version":"3.50.1"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T00:00:00Z","timestamp":1610928000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T00:00:00Z","timestamp":1610928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Plan","award":["2016YFD0901102"],"award-info":[{"award-number":["2016YFD0901102"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31472203, 31172342"],"award-info":[{"award-number":["31472203, 31172342"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the National Science and Technology Support Program of China","award":["2013BAD12B05"],"award-info":[{"award-number":["2013BAD12B05"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vesicular stomatitis (VS) is an acute, highly contagious and economically important zoonotic disease caused by the vesicular stomatitis virus (VSV). There is a need for effective and safe stable recombinant vaccine for the control of the disease. The human type 5 replication-defective adenovirus expression vector is a good way to construct recombinant vaccines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity. G proteins were successfully expressed with good immunogenicity. The rAds could induce the production of VSV antibodies in mice, and VSV neutralizing antibodies in goats, respectively. The neutralizing antibody titers could reach 1:32 in mice and 1:64 in goats. The rAds induced strong lymphocyte proliferation in mice and goats, which was significantly higher compared to the negative control groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The three rAds constructed in the study expressed VSV-G proteins and induced both humoral and cellular immune responses in mice and goats. These results lay the foundation for further studies on the use of rAds in vaccines expressing VSV-G.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-020-02740-6","type":"journal-article","created":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T15:11:18Z","timestamp":1610982678000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Recombinant adenovirus expressing vesicular stomatitis virus G proteins induce both humoral and cell-mediated immune responses in mice and goats"],"prefix":"10.1186","volume":"17","author":[{"given":"Xiaojuan","family":"Xue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaorong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shangjin","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6077-9499","authenticated-orcid":false,"given":"Gang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"issue":"1\u20132","key":"2740_CR1","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.jviromet.2009.12.005","volume":"164","author":"EJ Heo","year":"2010","unstructured":"Heo EJ, Lee HS, Jeoung HY, Ko HR, Kweon CH, Ko YJ. Development of a blocking ELISA using a recombinant glycoprotein for the detection of antibodies to vesicular stomatitis New Jersey virus. J Virol Methods. 2010;164(1\u20132):96\u2013100.","journal-title":"J Virol Methods"},{"issue":"3","key":"2740_CR2","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/S0749-0720(02)00031-2","volume":"18","author":"B Schmitt","year":"2002","unstructured":"Schmitt B. Vesicular stomatitis. Vet Clin North Am Food Anim Pract. 2002;18(3):453\u20139.","journal-title":"Vet Clin North Am Food Anim Pract"},{"issue":"5","key":"2740_CR3","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1136\/vr.i4075","volume":"179","author":"P Timoney","year":"2016","unstructured":"Timoney P. Vesicular stomatitis. Vet Rec. 2016;179(5):119\u201320.","journal-title":"Vet Rec"},{"issue":"4","key":"2740_CR4","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1603\/033.046.0419","volume":"46","author":"DG Mead","year":"2009","unstructured":"Mead DG, Lovett KR, Murphy MD, Pauszek SJ, Smoliga G, Gray EW, Noblet R, Overmyer J, Rodriguez LL. Experimental transmission of vesicular stomatitis New Jersey virus from Simulium vittatum to cattle: clinical outcome is influenced by site of insect feeding. J Med Entomol. 2009;46(4):866\u201372.","journal-title":"J Med Entomol"},{"issue":"10","key":"2740_CR5","doi-asserted-by":"publisher","first-page":"2269","DOI":"10.1080\/21645515.2019.1649532","volume":"15","author":"A Fathi","year":"2019","unstructured":"Fathi A, Dahlke C, Addo MM. Recombinant vesicular stomatitis virus vector vaccines for WHO blueprint priority pathogens. Hum Vaccin Immunother. 2019;15(10):2269\u201385.","journal-title":"Hum Vaccin Immunother"},{"issue":"2","key":"2740_CR6","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.jviromet.2007.09.010","volume":"147","author":"J Fernandez","year":"2008","unstructured":"Fernandez J, Aguero M, Romero L, Sanchez C, Belak S, Arias M, Sanchez-Vizcaino JM. Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay. J Virol Methods. 2008;147(2):301\u201311.","journal-title":"J Virol Methods"},{"issue":"1\u20132","key":"2740_CR7","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.jviromet.2011.12.010","volume":"180","author":"NP Ferris","year":"2012","unstructured":"Ferris NP, Clavijo A, Yang M, Velazquez-Salinas L, Nordengrahn A, Hutchings GH, Kristersson T, Merza M. Development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples. J Virol Methods. 2012;180(1\u20132):96\u2013100.","journal-title":"J Virol Methods"},{"issue":"6","key":"2740_CR8","doi-asserted-by":"publisher","first-page":"3578","DOI":"10.1128\/JVI.79.6.3578-3585.2005","volume":"79","author":"I Martinez","year":"2005","unstructured":"Martinez I, Wertz GW. Biological differences between vesicular stomatitis virus Indiana and New Jersey serotype glycoproteins: identification of amino acid residues modulating pH-dependent infectivity. J Virol. 2005;79(6):3578\u201385.","journal-title":"J Virol"},{"issue":"5","key":"2740_CR9","doi-asserted-by":"publisher","first-page":"608","DOI":"10.1177\/1040638713497945","volume":"25","author":"BJ McCluskey","year":"2013","unstructured":"McCluskey BJ, Pelzel-McCluskey AM, Creekmore L, Schiltz J. Vesicular stomatitis outbreak in the southwestern United States, 2012. J Vet Diagn Invest. 2013;25(5):608\u201313.","journal-title":"J Vet Diagn Invest"},{"issue":"29\u201330","key":"2740_CR10","doi-asserted-by":"publisher","first-page":"4035","DOI":"10.1016\/j.vaccine.2004.03.065","volume":"22","author":"I Martinez","year":"2004","unstructured":"Martinez I, Barrera JC, Rodriguez LL, Wertz GW. Recombinant vesicular stomatitis (Indiana) virus expressing New Jersey and Indiana glycoproteins induces neutralizing antibodies to each serotype in swine, a natural host. Vaccine. 2004;22(29\u201330):4035\u201343.","journal-title":"Vaccine"},{"issue":"17\u201318","key":"2740_CR11","doi-asserted-by":"publisher","first-page":"1932","DOI":"10.1016\/S0264-410X(03)00008-2","volume":"21","author":"JA House","year":"2003","unstructured":"House JA, House C, Dubourget P, Lombard M. Protective immunity in cattle vaccinated with a commercial scale, inactivated, bivalent vesicular stomatitis vaccine. Vaccine. 2003;21(17\u201318):1932\u20137.","journal-title":"Vaccine"},{"issue":"4","key":"2740_CR12","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1016\/j.prevetmed.2009.11.003","volume":"93","author":"AM Perez","year":"2010","unstructured":"Perez AM, Pauszek SJ, Jimenez D, Kelley WN, Whedbee Z, Rodriguez LL. Spatial and phylogenetic analysis of vesicular stomatitis virus over-wintering in the United States. Prev Vet Med. 2010;93(4):258\u201364.","journal-title":"Prev Vet Med"},{"key":"2740_CR13","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.virol.2013.10.025","volume":"449","author":"L Velazquez-Salinas","year":"2014","unstructured":"Velazquez-Salinas L, Pauszek SJ, Zarate S, Basurto-Alcantara FJ, Verdugo-Rodriguez A, Perez AM, Rodriguez LL. Phylogeographic characteristics of vesicular stomatitis New Jersey viruses circulating in Mexico from 2005 to 2011 and their relationship to epidemics in the United States. Virology. 2014;449:17\u201324.","journal-title":"Virology"},{"issue":"22","key":"2740_CR14","doi-asserted-by":"publisher","first-page":"2368","DOI":"10.1016\/S0264-410X(00)00007-4","volume":"18","author":"JD Cantlon","year":"2000","unstructured":"Cantlon JD, Gordy PW, Bowen RA. Immune responses in mice, cattle and horses to a DNA vaccine for vesicular stomatitis. Vaccine. 2000;18(22):2368\u201374.","journal-title":"Vaccine"},{"issue":"1","key":"2740_CR15","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S0304-4165(01)00195-7","volume":"1568","author":"H Mizuguchi","year":"2001","unstructured":"Mizuguchi H, Hayakawa T. Characteristics of adenovirus-mediated tetracycline-controllable expression system. Biochim Biophys Acta. 2001;1568(1):21\u20139.","journal-title":"Biochim Biophys Acta"},{"issue":"32","key":"2740_CR16","doi-asserted-by":"publisher","first-page":"3236","DOI":"10.1016\/j.vaccine.2013.05.016","volume":"31","author":"C Deal","year":"2013","unstructured":"Deal C, Pekosz A, Ketner G. Prospects for oral replicating adenovirus-vectored vaccines. Vaccine. 2013;31(32):3236\u201343.","journal-title":"Vaccine"},{"issue":"4","key":"2740_CR17","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.coviro.2011.07.009","volume":"1","author":"JC Small","year":"2011","unstructured":"Small JC, Ertl HC. Viruses - from pathogens to vaccine carriers. Curr Opin Virol. 2011;1(4):241\u20135.","journal-title":"Curr Opin Virol"},{"issue":"13","key":"2740_CR18","doi-asserted-by":"publisher","first-page":"4084","DOI":"10.1021\/bi952924i","volume":"35","author":"ME Mathieu","year":"1996","unstructured":"Mathieu ME, Grigera PR, Helenius A, Wagner RR. Folding, unfolding, and refolding of the vesicular stomatitis virus glycoprotein. Biochemistry. 1996;35(13):4084\u201393.","journal-title":"Biochemistry"},{"issue":"1\u20132","key":"2740_CR19","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.jviromet.2012.12.003","volume":"188","author":"C Sun","year":"2013","unstructured":"Sun C, Zhao K, Chen K, He W, Su G, Sun X, Wang L, Pan W, Zhang W, Gao F, Song D. Development of a convenient immunochromatographic strip for the diagnosis of vesicular stomatitis virus serotype Indiana infections. J Virol Methods. 2013;188(1\u20132):57\u201363.","journal-title":"J Virol Methods"},{"issue":"6869","key":"2740_CR20","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/415331a","volume":"415","author":"JW Shiver","year":"2002","unstructured":"Shiver JW, Fu TM, Chen L, Casimiro DR, Davies ME, Evans RK, Zhang ZQ, Simon AJ, Trigona WL, Dubey SA, Huang L, Harris VA, Long RS, Liang X, Handt L, Schleif WA, Zhu L, Freed DC, Persaud NV, Guan L, Punt KS, Tang A, Chen M, Wilson KA, Collins KB, Heidecker GJ, Fernandez VS, Perry HC, Joyce JG, Grimm KM, Cook JC, Keller PM, Kresock DS, Mach H, Troutman RD, Isopi LA, Williams DM, Xu Z, Bohannon KE, Volkin DB, Montefiori DC, Miura A, Krivulka GR, Lifton MA, Kuroda MJ, Schmitz JE, Letvin NL, Caulfield MJ, Bett AJ, Youil R, Kaslow DC, Emini EA. Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity. Nature. 2002;415(6869):331\u20135.","journal-title":"Nature"},{"issue":"11","key":"2740_CR21","doi-asserted-by":"publisher","first-page":"6305","DOI":"10.1128\/JVI.77.11.6305-6313.2003","volume":"77","author":"DR Casimiro","year":"2003","unstructured":"Casimiro DR, Chen L, Fu TM, Evans RK, Caulfield MJ, Davies ME, Tang A, Chen M, Huang L, Harris V, Freed DC, Wilson KA, Dubey S, Zhu DM, Nawrocki D, Mach H, Troutman R, Isopi L, Williams D, Hurni W, Xu Z, Smith JG, Wang S, Liu X, Guan L, Long R, Trigona W, Heidecker GJ, Perry HC, Persaud N, Toner TJ, Su Q, Liang X, Youil R, Chastain M, Bett AJ, Volkin DB, Emini EA, Shiver JW. Comparative immunogenicity in rhesus monkeys of DNA plasmid, recombinant vaccinia virus, and replication-defective adenovirus vectors expressing a human immunodeficiency virus type 1 gag gene. J Virol. 2003;77(11):6305\u201313.","journal-title":"J Virol"},{"key":"2740_CR22","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.vetmic.2017.10.020","volume":"212","author":"J Hernandez","year":"2017","unstructured":"Hernandez J, Rascon-Castelo E, Bray J, Lokhandwala S, Mwangi W. Immunogenicity of a recombinant adenovirus expressing porcine reproductive and respiratory syndrome virus polyepitopes. Vet Microbiol. 2017;212:7\u201315.","journal-title":"Vet Microbiol"},{"issue":"1","key":"2740_CR23","doi-asserted-by":"publisher","first-page":"112","DOI":"10.3382\/ps\/pey325","volume":"98","author":"AP Wang","year":"2019","unstructured":"Wang AP, Liu L, Gu LL, Guo CM, Wu S, Feng Q, Xia WL, Wu Z, Zhu SY. Protection against duck hepatitis a virus type 1 conferred by a recombinant avian adeno-associated virus. Poult Sci. 2019;98(1):112\u20138.","journal-title":"Poult Sci"},{"key":"2740_CR24","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.virol.2019.07.022","volume":"536","author":"X Wang","year":"2019","unstructured":"Wang X, Fang Z, Xiong J, Yang K, Chi Y, Tang X, Ma L, Zhang R, Deng F, Lan K, Zhou D. A chimpanzee adenoviral vector-based rabies vaccine protects beagle dogs from lethal rabies virus challenge. Virology. 2019;536:32\u20138.","journal-title":"Virology"},{"key":"2740_CR25","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.antiviral.2017.09.009","volume":"147","author":"Y Wu","year":"2017","unstructured":"Wu Y, Yang D, Xu B, Liang W, Sui J, Chen Y, Yang H, Chen H, Wei P, Qiao C. Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice. Antiviral Res. 2017;147:29\u201336.","journal-title":"Antiviral Res"},{"issue":"14","key":"2740_CR26","doi-asserted-by":"publisher","first-page":"8039","DOI":"10.1128\/JVI.77.14.8039-8047.2003","volume":"77","author":"I Martinez","year":"2003","unstructured":"Martinez I, Rodriguez LL, Jimenez C, Pauszek SJ, Wertz GW. Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host. J Virol. 2003;77(14):8039\u201347.","journal-title":"J Virol"},{"key":"2740_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetimm.2013.05.002","volume":"154","author":"Y Wang","year":"2013","unstructured":"Wang Y, Liu G, Chen Z, Li C, Shi L, Li W, Huang H, Tao C, Cheng C, Xu B, Li G. Recombinant adenovirus expressing F and H fusion proteins of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in goats. Vet Immunol Immunopathol. 2013;154:1\u20132):1\u20137.","journal-title":"Vet Immunol Immunopathol"},{"issue":"4","key":"2740_CR28","first-page":"406","volume":"55","author":"GP Boivin","year":"2016","unstructured":"Boivin GP, Bottomley MA, Grobe N. Responses of Male C57BL\/6N Mice to Observing the Euthanasia of Other Mice. J Am Assoc Lab Anim Sci. 2016;55(4):406\u201311.","journal-title":"J Am Assoc Lab Anim Sci"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-020-02740-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12917-020-02740-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-020-02740-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T15:11:27Z","timestamp":1610982687000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-020-02740-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,18]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["2740"],"URL":"https:\/\/doi.org\/10.1186\/s12917-020-02740-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-16879\/v1","asserted-by":"object"},{"id-type":"doi","id":"10.21203\/rs.3.rs-16879\/v2","asserted-by":"object"}]},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,18]]},"assertion":[{"value":"12 December 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 December 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 January 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All of the animal protocols were performed in accordance with the \u2018Guidelines for Experimental Animals\u2019 of the Ministry of Science and Technology (Beijing, China), and the study was approved by the Research Ethics Committee of the Chinese Academy of Agricultural Sciences (permit number: 2015\u2013014). No specific permits were required for these locations and activities. The owners gave their written consent for sample collection, and the locations where samples were collected are not privately owned or protected in any way.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"36"}}