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It is the most common cause of intramammary infections in dairy cows. Its public health importance increases inline to the continuous emergence of drug-resistant strains; such as Methicillin-resistant <jats:italic>S. aureus<\/jats:italic> (MRSA). Indeed, the recent emergence of human and veterinary adapted MRSA demands serious attention. The aim of this study was to determine the burden and drug resistance pattern of <jats:italic>S. aureus<\/jats:italic> in dairy farms in Mekelle and determine the molecular characteristics of MRSA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>This study was done on 385 lactating dairy cows and 71 dairy farmers. The ages of the cows and farmworkers were between 3 and 14 and 17\u201363\u2009years respectively. <jats:italic>S. aureus<\/jats:italic> was isolated from 12.5% of cows and 31% of farmworkers. Highest resistance was observed for penicillin (&gt;\u200990%) followed by tetracycline (32\u201335%) and trimethoprim-sulphamethoxazole (10\u201327%). But no resistance was observed for vancomycin, daptomycin, and rifampin. Only one isolate was MRSA both phenotypically and harboring <jats:italic>mecA<\/jats:italic>. This isolate was from nasal of a farmworker and was MRSA SCC<jats:italic>mec<\/jats:italic> Iva, <jats:italic>spa<\/jats:italic> type t064 of CC8. Multi-drug resistance was observed in 6.2% of cow isolates and 13.6% of nasal isolates.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>In this study, <jats:italic>S. aureus<\/jats:italic> infected 12.5% of dairy cows and colonized 31% of farmworkers. Except for penicillin, resistance to other drugs was rare. Although no MRSA was found from dairy cows the existence of the human and animal adapted and globally spread strain, MRSA SCC<jats:italic>mec<\/jats:italic> IVa spa t064, warrants for a coordinated action to tackle AMR in both human and veterinary in the country.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-020-2235-8","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T16:02:55Z","timestamp":1579708975000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Burden and antimicrobial resistance of S. aureus in dairy farms in Mekelle, Northern Ethiopia"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5170-4898","authenticated-orcid":false,"given":"Alem Abrha","family":"Kalayu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Asrat","family":"Woldetsadik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimtubezinash","family":"Woldeamanuel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wondwossen A.","family":"Gebreyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadesse","family":"Teferi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,22]]},"reference":[{"key":"2235_CR1","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1586\/ecp.10.121","volume":"3","author":"YC Lin","year":"2010","unstructured":"Lin YC, Peterson ML. 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