{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T13:31:42Z","timestamp":1780320702343,"version":"3.54.1"},"reference-count":12,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T00:00:00Z","timestamp":1581465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T00:00:00Z","timestamp":1581465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Porcilis\u00ae Ery+Parvo+Lepto is an octavalent inactivated ready-to-use vaccine that contains <jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic> (Ery), porcine parvovirus (PPV), and six serogroups of <jats:italic>Leptospira<\/jats:italic> (Lepto). The efficacy of Porcilis\u00ae Ery\u2009+\u2009Parvo+Lepto against reproductive problems associated with porcine parvovirus (PPV) infection was evaluated in pregnant gilts. For this, a group of ninegilts was vaccinated twice (at 5 and 6 months old) with Porcilis\u00ae Ery\u2009+\u2009Parvo+Lepto (Group 1), while a group of eight gilts was included as unvaccinated controls (Group 2). All pigs were artificially inseminated 4 weeks after the second vaccination. They were challenged during early gestation with PPV-27a, a virulent cluster D strain, and euthanized to collect their offspring by hysterectomy around day 90 in pregnancy. Antibody responses against PPV in gilts were measured, and the presence of PPV in progeny was also determined.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>No clinical signs were observed after vaccination. After PPV challenge, all foetuses from the vaccinated gilts were alive (132\/132), while in the unvaccinated group only 41% were alive (46\/112), 19.6% were dead and 39.4% of the offspring (44\/112) were mummified. PPV could be detected by qPCR in 14% of the progeny from vaccinated gilts at an average of 4.7 log<jats:sub>10<\/jats:sub>\/ml, whereas this was significantly higher in the control group, where 90% of the progeny were PPV positive, with titres of 9.8 log<jats:sub>10<\/jats:sub>\/ml on average.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The present study demonstrates that vaccination of gilts with Porcilis\u00ae Ery\u2009+\u2009Parvo+Lepto was safe and induced an immune response sufficient to protect progeny against PPV by reducing transplacental infection.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-020-2272-3","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T16:07:16Z","timestamp":1581523636000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["An octavalent vaccine provides pregnant gilts protection against a highly virulent porcine parvovirus strain"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0341-2874","authenticated-orcid":false,"given":"Erwin","family":"van den Born","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul P. M.","family":"van den Elzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"van Kilsdonk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu J. H.","family":"Hoeijmakers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruud P. A. M.","family":"Segers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,12]]},"reference":[{"key":"2272_CR1","doi-asserted-by":"publisher","first-page":"393","DOI":"10.3390\/v9120393","volume":"9","author":"I M\u00e9sz\u00e1ros","year":"2017","unstructured":"M\u00e9sz\u00e1ros I, Olasz F, Cs\u00e1gola A, Tijssen P, Z\u00e1dori Z. Biology of porcine parvovirus (ungulate parvovirus). Viruses. 2017;9:393.","journal-title":"Viruses"},{"key":"2272_CR2","first-page":"1768","volume-title":"Fields virology","author":"K Berns","year":"2013","unstructured":"Berns K, Parrish CR. Parvoviridae. In: Knipe DM, Howley PM, editors. Fields virology. 6th ed. Philadelphia: Lippincott Williams & Wilkins; 2013. p. 1768\u201391.","edition":"6"},{"key":"2272_CR3","doi-asserted-by":"publisher","first-page":"2628","DOI":"10.1099\/vir.0.033662-0","volume":"92","author":"AF Streck","year":"2011","unstructured":"Streck AF, Bonatto SL, Homeier T, Souza CK, Gon\u00e7alves KR, Gava D, Canal CW, Truyen U. High rate of viral evolution in the capsid protein of porcine parvovirus. J Gen Virol. 2011;92:2628\u201336.","journal-title":"J Gen Virol"},{"key":"2272_CR4","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1099\/vir.0.82302-0","volume":"88","author":"EJL Zeeuw","year":"2007","unstructured":"Zeeuw EJL, Leinecker N, Herwig V, Selbitz HJ, Truyen U. Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts. J Gen Virol. 2007;88:420\u20137.","journal-title":"J Gen Virol"},{"key":"2272_CR5","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1099\/vir.0.81086-0","volume":"87","author":"P Zimmermann","year":"2006","unstructured":"Zimmermann P, Ritzmann M, Selbitz HJ, Heinritzi K, Truyen U. VP1 sequences of German porcine parvovirus isolates define two genetic lineages. J Gen Virol. 2006;87:295\u2013301.","journal-title":"J Gen Virol."},{"key":"2272_CR6","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.meegid.2015.10.007","volume":"36","author":"AF Streck","year":"2015","unstructured":"Streck AF, Canal CW, Truyen U. Molecular epidemiology and evolution of porcine parvoviruses. Infect Genet Evol. 2015;36:300\u20136.","journal-title":"Infect Genet Evol"},{"key":"2272_CR7","first-page":"475","volume-title":"Diseases of Swine","author":"RL Wood","year":"1992","unstructured":"Wood RL. Erysipelas. In: Leman AD, Straw BE, Mengeling WL, D\u2019Allaire S, Taylor DJ, editors. Diseases of Swine. 7th ed. Iowa State: Univ. Press, Ames, IA; 1992. p. 475\u201386.","edition":"7"},{"key":"2272_CR8","volume-title":"In: straw BE, Zimmerman JJ, D'Allaire S, Taylor DJ, editors. Leptospirosis, chapter 41, diseases of swine. 9th ed","author":"WA Ellis","year":"2006","unstructured":"Ellis WA. In: straw BE, Zimmerman JJ, D'Allaire S, Taylor DJ, editors. Leptospirosis, chapter 41, diseases of swine. 9th ed. Ames, IA: Blackwell Publishing; 2006."},{"key":"2272_CR9","doi-asserted-by":"publisher","first-page":"3963","DOI":"10.1016\/j.vaccine.2015.06.048","volume":"33","author":"AAC Jacobs","year":"2015","unstructured":"Jacobs AAC, Harks F, Hoeijmakers M, Collell M, Segers RPAM. Safety and efficacy of a new octavalent combined erysipelas, Parvo and Leptospira vaccine in gilts against Leptospira interrogans serovars Pomona associated disease and foetal death. Vaccine. 2015;33:3963\u20139.","journal-title":"Vaccine."},{"key":"2272_CR10","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s40813-015-0011-0","volume":"1","author":"AAC Jacobs","year":"2015","unstructured":"Jacobs AAC, Harks F, Hoeijmakers M, Segers RPAM. A novel octavalent combined erysipelas, Parvo and Leptospira vaccine provides (cross) protection against infection following challenge of pigs with 9 different Leptospira interrogans serovars. Porcine Health Manag. 2015;1:16.","journal-title":"Porcine Health Manag"},{"key":"2272_CR11","doi-asserted-by":"publisher","first-page":"2437","DOI":"10.1099\/vir.0.012054-0","volume":"90","author":"A J\u00f3\u017awik","year":"2009","unstructured":"J\u00f3\u017awik A, Manteufel J, Selbitz HJ, Truyen U. Vaccination against porcine parvovirus protects against disease, but does not prevent infection and virus shedding after challenge infection with a heterologous virus strain. J Gen Virol. 2009;90:2437\u201341.","journal-title":"J Gen Virol."},{"key":"2272_CR12","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.1099\/jgv.0.000446","volume":"97","author":"T Foerster","year":"2016","unstructured":"Foerster T, Streck AF, Speck S, Selbitz HJ, Lindner T, Truyen U. An inactivated whole-virus porcine parvovirus vaccine protects pigs against disease but does not prevent virus shedding even after homologous virus challenge. J Gen Virol. 2016;97:1408\u201313.","journal-title":"J Gen Virol."}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-020-2272-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12917-020-2272-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-020-2272-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T09:35:07Z","timestamp":1613036107000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-020-2272-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,12]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["2272"],"URL":"https:\/\/doi.org\/10.1186\/s12917-020-2272-3","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,12]]},"assertion":[{"value":"5 July 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 February 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study was approved after ethical evaluation by the Animal Welfare Body of MSD Animal Health. The committee\u2019s reference number for this study is EPD 14.053.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All authors are employed by MSD Animal Health.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"55"}}