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The objectives of this study were to investigate antibody responses to porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus (IAV), and<jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>(<jats:italic>M. hyopneumoniae<\/jats:italic>) from weaning to the end of the finisher stage on a subset of commercial swine farms in Ontario, Canada, and to examine the association between nursery diet and antibody responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, older pigs were more likely to test seropositive for PRRSV and less likely to test seropositive for<jats:italic>M. hyopneumoniae<\/jats:italic>(<jats:italic>p<\/jats:italic>\u2009&lt;\u2009\u00a00.001). Pigs were more likely to test seropositive for IAV at weaning and the end of the grower and finisher stages compared to the end of nursery (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009\u00a00.001). Pigs that were seropositive for IAV were more likely to test seropositive for both PRRSV and<jats:italic>M. hyopneumoniae<\/jats:italic>(<jats:italic>p<\/jats:italic>\u2009&lt;\u2009\u00a00.001). Two, 9, and 4 groups that had more than 20% of pigs seropositive to PRRSV, IAV, and<jats:italic>M. hyopneumoniae<\/jats:italic>, respectively, from the end of nursery to the end of finisher were classified as seropositive. Pigs fed a plant-based (low complexity) diet during nursery were more likely to be seropositive for PRRSV (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009\u00a00.001) but there were no significant differences in seropositivity to IAV or<jats:italic>M. hyopneumoniae<\/jats:italic>due to nursery diet complexity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study provides information regarding changes in serum antibody in pigs across different stages of production and highlights periods of vulnerability. Additionally, these findings may encourage further research into the effects of nursery diet complexity on disease susceptibility and immune response.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-021-02756-6","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T08:30:23Z","timestamp":1613637023000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antibody responses to porcine reproductive and respiratory syndrome virus, influenza A virus, and Mycoplasma hyopneumoniae from weaning to the end of the finisher stage in fourteen groups of pigs in Ontario, Canada"],"prefix":"10.1186","volume":"17","author":[{"given":"Elana","family":"Raaphorst","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9612-0263","authenticated-orcid":false,"given":"Abdolvahab","family":"Farzan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Friendship","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon N.","family":"Lillie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"2756_CR1","volume-title":"AASV Annual Meeting","author":"C Haden","year":"2012","unstructured":"Haden C, Painter T, Fangman T, Holtkamp D. 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