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All strains were tested for their antibiotic susceptibility by disk diffusion method. Simplex and multiplex PCR were performed to identify the \u03b2-lactams antibiotic resistance genes (<jats:italic>bla<\/jats:italic><jats:sub>NMD<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>OXA\u221248<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>VIM<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>IMP<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>CTX\u2212M<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>), sulfonamides (Sul1, Sul2), tetracyclines (TetA, TetB, TetC, TetD, TetM), aminoglycosides (aac3Ia), vancomycin (VanA, VanB, VanC), macrolides (ermA, ermB, ermC) as well as the plasmid-mediated quinolone resistance (PMQR) markers (QnrA, QnrB, QnrS), and class 1\u00a0integrons (Int1, drfA1-aadA1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The<jats:italic>Enterococcus<\/jats:italic>spp. isolates exhibited phenotypic resistance to vancomycin (35\u2009%) and macrolides (erythromycin) (75\u2009%); from the vancomycin \u2013\u00a0resistant strains, 5\u2009% harboured VanA (<jats:italic>E. faecalis<\/jats:italic>), while the erythromycin resistant isolates were positive for the ermA gene (<jats:italic>E. faecalis<\/jats:italic>\u2212\u200910\u2009%,<jats:italic>E. faecium<\/jats:italic>\u2212\u20095\u2009%). The Gram- negative rods (GNR) exhibited a high level of resistance to \u03b2-lactams: cefuroxime (63\u2009%), cefazolin (42\u2009%), ceftriaxone (8\u2009%), ceftazidime and aztreonam (4\u2009% each). The genetic determinants for beta-lactam resistance were represented by<jats:italic>bla<\/jats:italic><jats:sub>CTX\u2212M\u2212like<\/jats:sub>(33\u2009%),<jats:italic>bla<\/jats:italic><jats:sub>NDM\u2212like<\/jats:sub>and<jats:italic>bla<\/jats:italic><jats:sub>IMP\u2212like<\/jats:sub>(8.33\u2009%) genes. The resistance to non-\u03b2-lactam antibiotics was ascertained to the following genes: quinolones (QnrS \u2212\u20094.16\u2009%); sulfonamides (Sul1\u201375\u2009%, Sul2\u20134.16\u2009%); aminoglycosides (aac3Ia \u2212\u20094.16\u2009%); tetracyclines (tetA \u2013 25\u2009%, tetC \u2212\u200915\u2009%). The integrase gene was found in more than 50\u2009% of the studied strains (58.33\u2009%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The cultivable aquatic microbiota from fishery lakes is dominated by enterococci and<jats:italic>Enterobacterales<\/jats:italic>strains. The GNR strains exhibited high levels of \u03b2-lactam resistance mediated by extended spectrum beta-lactamases and metallo-\u03b2-lactamases. The<jats:italic>Enterococcus<\/jats:italic>sp. isolates were highly resistant to macrolides and vancomycin. The high level and diversity of resistance markers, correlated with a high frequency of integrons is suggesting that this environment could act as an important reservoir of antibiotic resistance genes with a great probability to be horizontally transmitted to other associated species from the aquatic sediments microbiota, raising the potential zoonotic risk for fish consumers.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-021-02770-8","type":"journal-article","created":{"date-parts":[[2021,1,26]],"date-time":"2021-01-26T12:18:32Z","timestamp":1611663512000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antibiotic resistance profiles in cultivable microbiota isolated from some romanian natural fishery lakes included in Natura 2000 network"],"prefix":"10.1186","volume":"17","author":[{"given":"Veronica","family":"Laz\u0103r","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Gheorghe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Curutiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana","family":"Savin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florica","family":"Marinescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violeta Corina","family":"Cristea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dumitru","family":"Dobre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Loredana","family":"Popa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Carmen","family":"Chifiriuc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mircea Ioan","family":"Popa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,26]]},"reference":[{"key":"2770_CR1","doi-asserted-by":"crossref","unstructured":"Zhang XX, Zhang T, Fang HHP. 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