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The maximal load for a horse\u2019s back during riding has been suggested not to exceed 20% of the horses\u2019 body weight. The common prevalence of back problems in riding horses prompted the popularization of thermography of the thoracolumbar region. However, the analysis methods of thermographic images used so far do not distinguish loaded horses with body weight varying between 10 and 20%.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The superficial body temperature (SBT) of the thoracolumbar region of the horse\u2019s back was imaged using a non-contact thermographic camera before and after riding under riders with LBW (low body weight, 10%) and HBW (high body weight, 15%). Images were analyzed using six methods: five recent SBT analyses and the novel approach based on Gray Level Co-Occurrence Matrix (GLCM) and Gray Level Run Length Matrix (GLRLM). Temperatures of the horse\u2019s thoracolumbar region were higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) after then before the training, and did not differ depending on the rider\u2019s body weight (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05), regardless of used SBT analysis method. Effort-dependent differences (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) were noted for six features of GLCM and GLRLM analysis. The values of selected GLCM and GLRLM features also differed (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) between the LBW and HBW groups.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The GLCM and GLRLM analyses allowed the differentiation of horses subjected to a load of 10 and 15% of their body weights while horseback riding in contrast to the previously used SBT analysis methods. Both types of analyzing methods allow to differentiation thermal images obtained before and after riding. The textural analysis, including selected features of GLCM or GLRLM, seems to be promising tools in considering the quantitative assessment of thermographic images of horses\u2019 thoracolumbar region.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-021-02803-2","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T11:04:10Z","timestamp":1614683050000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A novel approach to thermographic images analysis of equine thoracolumbar region: the effect of effort and rider\u2019s body weight on structural image complexity"],"prefix":"10.1186","volume":"17","author":[{"given":"Malgorzata","family":"Masko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Borowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9436-1074","authenticated-orcid":false,"given":"Malgorzata","family":"Domino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Jasinski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukasz","family":"Zdrojkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zdzislaw","family":"Gajewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,2]]},"reference":[{"issue":"1","key":"2803_CR1","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/s0749-0739(17)30077-9","volume":"17","author":"TA Turner","year":"2001","unstructured":"Turner TA. 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The ethics approval was deemed unnecessary according to regulations of the II Local Ethical Committee on Animal Testing in Warsaw and the National Ethical Committees on Animal Testing, as well as according to Polish legal regulations (Ustawa z dnia 15 stycznia 2015 r. o ochronie zwierzat wykorzystywanych do cel\u00f3w naukowych lub edukacyjnych, Dz.U.2018.0.1207 (Resolution on the animals protection used for scientific and educational purposes), because all procedures in the study were non-invasive and did not cause distress and pain equal to or greater then a needlestick.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no conflicts of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"99"}}