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The objectives of this study were to better understand antimicrobial use in pigs, beef cattle, and poultry on farms on Guadeloupe, French West Indies, and to acquire data on AMR in\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      in these food-producing animals. A cross-sectional survey was conducted at 45 farms on Guadeloupe, and practical use of antimicrobials was documented in declarative interviews between March and July 2018. A total of 216 fecal samples were collected between January 2018 and May 2019, comprising 124 from pigs, 75 from beef cattle, and 17 from poultry litter.\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates were obtained for further testing by isolation and identification from field samples. Antimicrobial susceptibility testing and screening for\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX-M<\/jats:sub>\n                      ,\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>TEM<\/jats:sub>\n                      ,\n                      <jats:italic>tet<\/jats:italic>\n                      A, and\n                      <jats:italic>tet<\/jats:italic>\n                      B resistance genes by polymerase chain reaction on extracted genomic DNA were performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The study showed rational use of antimicrobials, consisting of occasional use for curative treatment by veterinary prescription. Tetracycline was the most commonly used antimicrobial, but its use was not correlated to\n                      <jats:italic>E. coli<\/jats:italic>\n                      resistance. Extended-spectrum \u03b2-lactamase (ESBL)\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates were detected in 7.3% of pigs, 14.7% of beef cattle, and 35.3% of poultry.\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX-M-1<\/jats:sub>\n                      was the predominant gene found in ESBL-\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates (68.8%), followed by\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX-M-15<\/jats:sub>\n                      (31.3%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Despite rational use of antimicrobials, the rate of ESBL-\n                      <jats:italic>E. coli<\/jats:italic>\n                      in food-producing animals in Guadeloupe, although moderate, is a concern. Further studies are in progress to better define the genetic background of the ESBL-\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-021-02810-3","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T07:02:51Z","timestamp":1615186971000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Antimicrobial use and resistance in Escherichia coli from healthy food-producing animals in Guadeloupe"],"prefix":"10.1186","volume":"17","author":[{"given":"Ga\u00eblle","family":"Gruel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arantxa","family":"Sellin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Riveiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Pot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Breurec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Guyomard-Rabenirina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Talarmin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8258-5089","authenticated-orcid":false,"given":"S\u00e9verine","family":"Ferdinand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"2810_CR1","unstructured":"O\u2019Neill J. 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Furthermore, the project was considered to be outside the scope of the regulations on animal experimentation by the chairman of the Ethics Committee on animal experimentation of the Antilles and Guyane (registered with the French Ministry of Higher Education, Research and Innovation No. 69). Thus, according to French national law for the protection of animals (No. 2013\u2013118), which reproduces European directive 2010\/63\/UE on the protection of animals used for experimental and other scientific purposes, no ethics committee approval was deemed necessary according to Article 7.1 on recommendations for animal welfare and Article 7.8 on use of animals in research and education of the World Organization for Animal Health Terrestrial Animal Health Code, used in France. The entity responsible for the animals was the slaughterhouse, and authorization for sample collection was obtained from the director of the slaughterhouse. Sampling of pig, beef cattle, and chicken feces was authorized verbally by the owners, who are responsible for the animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"116"}}