{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:17:39Z","timestamp":1794003459440,"version":"4.8.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,3,20]],"date-time":"2021-03-20T00:00:00Z","timestamp":1616198400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,3,20]],"date-time":"2021-03-20T00:00:00Z","timestamp":1616198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>A novel <jats:italic>Brucella<\/jats:italic> strain closely related to <jats:italic>Brucella<\/jats:italic> (<jats:italic>B<\/jats:italic>.) <jats:italic>melitensis<\/jats:italic> biovar (bv) 3 was found in Croatian cattle during testing within a brucellosis eradication programme.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Case presentation<\/jats:title>\n                <jats:p>Standardised serological, brucellin skin test, bacteriological and molecular diagnostic screening for <jats:italic>Brucella<\/jats:italic> infection led to positive detection in one dairy cattle herd. Three isolates from that herd were identified to species level using the Bruce ladder method. Initially, two strains were typed as <jats:italic>B. melitensis<\/jats:italic> and one as <jats:italic>B. abortus<\/jats:italic>, but multiplex PCR based on IS<jats:italic>711<\/jats:italic> and the Suis ladder showed that all of them to belong to <jats:italic>B. melitensis,<\/jats:italic> and the combination of whole-genome and multi-locus sequencing as well as Multi-Locus Variable numbers of tandem repeats Analysis (MLVA) highlighted a strong proximity within the phylogenetic branch of <jats:italic>B. melitensis<\/jats:italic> strains previously isolated from Croatia, Albania, Kosovo and Bosnia and Herzegovina. Two isolates were determined to be <jats:italic>B. melitensis<\/jats:italic> bv. 3, while the third showed a unique phylogenetic profile, growth profile on dyes and bacteriophage typing results. This isolate contained the 609-bp <jats:italic>omp31<\/jats:italic> sequence, but not the 723-bp <jats:italic>omp31<\/jats:italic> sequence present in the two isolates of <jats:italic>B. melitensis<\/jats:italic> bv. 3.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Identification of a novel <jats:italic>Brucella<\/jats:italic> variant in this geographic region is predictable given the historic endemicity of brucellosis. The emergence of a new variant may reflect a combination of high prevalence among domestic ruminants and humans as well as weak eradication strategies. The zoonotic potential, reservoirs and transmission pathways of this and other <jats:italic>Brucella<\/jats:italic> variants should be explored.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-021-02833-w","type":"journal-article","created":{"date-parts":[[2021,3,20]],"date-time":"2021-03-20T20:02:41Z","timestamp":1616270561000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["New Brucella variant isolated from Croatian cattle"],"prefix":"10.1186","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8317-5831","authenticated-orcid":false,"given":"Silvio","family":"Spicic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Zdelar-Tuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Ponsart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene S.","family":"Hendriksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Reil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Girault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pimlapas","family":"Leekitcharoenphon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vesna","family":"Rukavina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Rubin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Freddi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Duvnjak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,20]]},"reference":[{"key":"2833_CR1","unstructured":"Manual of Diagnostic Tests and Vaccinesfor Terrestrial Animals 2019 (OIE): Brucellosis (Brucella abortus, B. melitensis and B. suis) (infection with B. abortus, B. melitensis and B. suis): https:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/3.01.04_BRUCELLO-SIS.pdf. 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