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These infections have led to substantial economic losses in the Chinese swine industry.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>This study, constructed a novel recombinant vaccine strain with gI\/gE deletion (PRV-GD2013-\u0394gI\/gE) by overlapping PCR and homologous recombination technology. The growth curves and plaque morphology of the recombinant virus were similar to those of the parental strain. However, PRV-GD2013-\u0394gI\/gE infection was significantly attenuated in mice compared with that of PRV-GD2013. Two-week-old piglets had normal rectal temperatures and displayed no clinical symptoms after being inoculated with 10<jats:sup>5<\/jats:sup> TCID<jats:sub>50<\/jats:sub> PRV-GD2013-\u0394gI\/gE, indicating that the recombinant virus was avirulent in piglets. Piglets were immunized with different doses of PRV-GD2013-\u0394gI\/gE, or a single dose of Bartha-K61 or DMEM, and infected with PRV-GD2013 at 14\u2009days post-vaccination. Piglets given high doses of PRV-GD2013-\u0394gI\/gE showed no obvious clinical symptoms, and their antibody levels were higher than those of other groups, indicating that the piglets were completely protected from PRV-GD2013.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The PRV-GD2013-\u0394gI\/gE vaccine strain could be effective for immunizing Chinese swine herds against the pseudorabies virus (PRV) strain.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-021-02861-6","type":"journal-article","created":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T20:37:57Z","timestamp":1618432677000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant pseudorabies virus with gI\/gE deletion generated by overlapping polymerase chain reaction and homologous recombination technology induces protection against the PRV variant PRV-GD2013"],"prefix":"10.1186","volume":"17","author":[{"given":"Wenhui","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dijing","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengpo","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erpeng","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoming","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinding","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6960-9354","authenticated-orcid":false,"given":"Mingqiu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,14]]},"reference":[{"issue":"1\u20132","key":"2861_CR1","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0378-1135(96)01353-3","volume":"56","author":"A Jons","year":"1997","unstructured":"Jons A, Gerdts V, Lange E, Kaden V, Thomas C. 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All procedures involving animals were conducted in compliance with the guidelines of the Animal Welfare Act and the Guide for the Care and Use of Laboratory Animals of South China Agricultural University (Guangzhou, China, Approval No. SYXK [Yue] 2014\u20130136).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"164"}}