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At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant L. casei-OMP16-PEDVS strain expressing PEDVS protein of PEDV and OMP16 protein of<jats:italic>Brucella abortus<\/jats:italic>strain. To know the immunogenicity of the recombinant L. casei-OMP16-PEDVS candidate vaccine, it was compared with BL21-OMP16-PEDVS-F, BL21-OMP16-PEDVS, and BL21-PEDVS recombinant protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that we could detect higher levels of IgG, neutralizing antibody, IL-4, IL-10, and INF-\u03b3 in serum and IgA in feces of L. casei-OMP16-PEDVS immunized mice, which indicated that L. casei-OMP16-PEDVS candidate vaccine could induce higher levels of humoral immunity, cellular immunity, and mucosal immunity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Therefore, L. casei-OMP16-PEDVS is a promising candidate vaccine for prophylaxis of PEDV infection.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-021-02885-y","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T20:03:06Z","timestamp":1620158586000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice"],"prefix":"10.1186","volume":"17","author":[{"given":"Xiaowen","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingzhou","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dasheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"2885_CR1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.virusres.2016.05.026","volume":"226","author":"D Wang","year":"2016","unstructured":"Wang D, Fang L, S X. 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All procedures followed the instruction of the care and use of laboratory animals provided by Shandong provincial public service facilities. The authors state their agreement to publication ethics. This study does not include any experiment performed on human participants.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors have agreed to publish all the identifiable images and identifiers in the paper.Availability of data and materials: All data generated or analysed during this study are included in this published article and its supplementary information files.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors have declared that no competing financial interests exist.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"184"}}