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Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g \u00b1 0.50<jats:italic>, n<\/jats:italic> = 240) were randomly allocated into 4 experimental groups (6 replicates\/group and 10 chicks\/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>During the starter period, dietary GLM and oregano EO did not show significant (<jats:italic>P &gt;<\/jats:italic> 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and relative growth rate (RGR), and a linear decrease in the FCR at 0.75% dietary level of GLM and oregano EO compared to the control. The broken-line regression model showed that the optimum dietary level of GLM and oregano EO blend was 0.58% based on final BW and FCR. The 0.45% or 0.15% dietary level of supplemented additives lowered (<jats:italic>P<\/jats:italic> &lt; 0.05) the AID% of threonine and arginine, respectively, with no change in the AID% of other assessed amino acids at all dietary levels. Muscle thickness in jejunum and ileum in all dietary supplemented groups was increased (<jats:italic>P<\/jats:italic> &lt; 0.05); however, such increase (<jats:italic>P<\/jats:italic> &lt; 0.05) in the duodenum was shown at 0.45 and 0.75% dietary levels. All GLM and oregano EO supplemented groups showed increased (<jats:italic>P<\/jats:italic> &lt; 0.05) duodenal, jejunal, and ileal villus height. The 0.15 and\/or 0.75% dietary levels of supplemented additives increased (<jats:italic>P<\/jats:italic> &lt; 0.05) the ileal and duodenal crypt depth, respectively, with a decreased (<jats:italic>P<\/jats:italic> &lt; 0.05) duodenal crypt depth at 0.15% dietary level. The goblet cell count in ileum decreased (<jats:italic>P<\/jats:italic> &lt; 0.05) in all GLM and oregano EO supplemented groups, but this decreased count (<jats:italic>P<\/jats:italic> &lt; 0.05) was detected in jejunum at 0.45 and 0.75% dietary levels. The GLM and oregano EO supplemented groups did not show significant (<jats:italic>P &gt;<\/jats:italic> 0.05) changes in the assessed metabolic and immune status parameters. Economically, the total return and performance index was increased at 0.75% dietary level.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Better growth performance was achieved at a 0.75 % dietary level of GLM and oregano EO by improving most intestinal morphometric measures. The optimum dietary level detected was 0.58%. The lack of influence of supplemented additives on chickens' immune and metabolic responses could indicate a lack of synergy between GLM and oregano EO.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-021-03022-5","type":"journal-article","created":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T16:02:25Z","timestamp":1632585745000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens"],"prefix":"10.1186","volume":"17","author":[{"given":"Shimaa A.","family":"Amer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samar A.","family":"Tolba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina M. 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