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Medication is a general approach for the control of <jats:italic>M. synoviae<\/jats:italic> infection, but antibiotics are sometimes ineffective in clinical practice. To investigate the sensitivity of <jats:italic>M. synoviae<\/jats:italic> to antimicrobials commonly used in the treatment of <jats:italic>M. synoviae<\/jats:italic> infection, the antibiotic susceptibility of 32\u2009<jats:italic>M. synoviae<\/jats:italic> strains isolated from China from 2016 to 2019 were determined using the minimum inhibitory concentration (MIC) method.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>All isolates had low MIC values for the combination of lincomycin and spectinomycin, pleuromutilin, and macrolides. However, the <jats:italic>M. synoviae<\/jats:italic> isolates displayed variance in MICs for doxycycline hydrochloride with a range of 0.25 to 8\u2009\u03bcg\/mL, and oxytetracycline hydrochloride with a range of 0.5 to 8\u2009\u03bcg\/mL. Three and one <jats:italic>M. synoviae<\/jats:italic> isolates showed intermediate MIC values to doxycycline hydrochloride and oxytetracycline hydrochloride, respectively. High MIC values for enrofloxacin were detected in all isolates with MICs ranging from 4 to 32\u2009\u03bcg\/mL. Furthermore, comparison of the <jats:italic>parC<\/jats:italic> QRDR identified a mutation at nucleotide position 254 (C254T) resulting in a Thr 85 Ile amino acid change in all <jats:italic>M. synoviae<\/jats:italic> isolates and the reference strain ATCC 25204 being resistant to enrofloxacin. Moreover, mutations at Glu 804 Gly and Thr 686 Ala of <jats:italic>gyrA<\/jats:italic> QRDR were identified in all <jats:italic>M. synoviae<\/jats:italic> isolates and ATCC 25204. The mutation in the QRDR of the <jats:italic>parE<\/jats:italic> gene resulted in amino acid changes at positions 197 (Pro to Ser) in 27\/32\u2009<jats:italic>M. synoviae<\/jats:italic> isolates.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Three nonsynonymous mutations in <jats:italic>gyrA<\/jats:italic> and <jats:italic>parE<\/jats:italic> were first identified to be related to enrofloxacin resistance. Our results showed that <jats:italic>M. synoviae<\/jats:italic> resistance to enrofloxacin is widespread<jats:italic>.<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-021-03104-4","type":"journal-article","created":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T06:02:41Z","timestamp":1641189761000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Antibiotic resistance of Mycoplasma Synoviae strains isolated in China from 2016 to 2019"],"prefix":"10.1186","volume":"18","author":[{"given":"Xiaorong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjiao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhong","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yantao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,3]]},"reference":[{"issue":"5","key":"3104_CR1","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.3382\/ps.0521909","volume":"52","author":"TH Vardaman","year":"1973","unstructured":"Vardaman TH, Reece FN, Deaton JW. 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