{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T05:05:20Z","timestamp":1792213520201,"version":"4.4.0"},"reference-count":9,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T00:00:00Z","timestamp":1641168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T00:00:00Z","timestamp":1641168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100009947","name":"merck sharp and dohme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Mannheimia haemolytica<\/jats:italic> is commonly associated with respiratory disease in cattle worldwide as a cause of fibrinous pneumonia, bronchopneumonia and pleuritis. <jats:italic>M. haemolytica<\/jats:italic> is further subdivided into 12 serovars, however not all are considered to be pathogenic in cattle. The study aim was to determine the most common serovars of <jats:italic>M. haemolytica<\/jats:italic> associated with respiratory disease in cattle in Great Britain, which is currently unknown and could be useful information for clinicians when considering preventative strategies.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>One hundred four\u2009<jats:italic>M. haemolytica<\/jats:italic> isolates isolated from bovine clinical pathology and post-mortem samples from pneumonia cases between 2016 and 2018 were tested using a multiplex PCR assay to identify <jats:italic>M. haemolytica<\/jats:italic> serovars A1, A2 and A6. 46 isolates (44.2%) typed as <jats:italic>M. haemolytica<\/jats:italic> serovar A1, 31 (29.8%) as <jats:italic>M. haemolytica<\/jats:italic> serovar A2 and 18 isolates (17.3%) as <jats:italic>M. haemolytica<\/jats:italic> serovar A6. Nine isolates (8.7%) were not A1, A2 or A6 so were considered to belong to other serovars or were not typable.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study highlights the importance of <jats:italic>M. haemolytica<\/jats:italic> serovars other than A1 which may be responsible for respiratory disease in cattle and could help guide the veterinarian when making choices on preventative vaccination programmes.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-021-03121-3","type":"journal-article","created":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T07:03:20Z","timestamp":1641193400000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Mannheimia haemolytica serovars associated with respiratory disease in cattle in Great Britain"],"prefix":"10.1186","volume":"18","author":[{"given":"Colin","family":"Mason","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Errington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Foster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Thacker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Grace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9476-7322","authenticated-orcid":false,"given":"Katharine","family":"Baxter-Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,3]]},"reference":[{"key":"3121_CR1","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1017\/S1466252307001375","volume":"8","author":"JA Rice","year":"2008","unstructured":"Rice JA, Carrasco-Medina L, Hodgins DC, Shewen PE. Mannheimia haemolytica and bovine respiratory disease. Anim Health Res Rev. 2008;8:117\u201328.","journal-title":"Anim Health Res Rev"},{"key":"3121_CR2","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1099\/00207713-49-1-67","volume":"49","author":"\u00d8 Angen","year":"1999","unstructured":"Angen \u00d8, Mutters R, Caugant DA, Olsen JE, Bisguard M. Taxonomic relationships of the (Pasteurella haemolytica) complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. Nov., comb. nov., Mannheimia granulomatis sp. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov., and Mannheimia varigena sp. nov. Int J Syst Bacteriol. 1999;49:67\u201386.","journal-title":"Int J Syst Bacteriol"},{"key":"3121_CR3","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1099\/ijs.0.64521-0","volume":"57","author":"PJ Blackall","year":"2007","unstructured":"Blackall PJ, Bojesen AM, Christensen H, Bisguaard M. Reclassification of (Pasteurella trehalosi) as Bibersteinia trehalosi. Int J Syst Evol Microbiol. 2007;57:666\u201374.","journal-title":"Int J Syst Evol Microbiol"},{"key":"3121_CR4","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1177\/104063870001200617","volume":"12","author":"GM Al-Ghamdi","year":"2000","unstructured":"Al-Ghamdi GM, Ames TR, Baker JC, Walker R, Chase CCL, Frank GH, et al. Serotyping of Mannheimia (Pasteurella) haemolytica isolates from the upper Midwest United States. J Vet Diag Invest. 2000;12:576\u20138.","journal-title":"J Vet Diag Invest"},{"key":"3121_CR5","first-page":"38","volume":"78","author":"CL Klima","year":"2014","unstructured":"Klima CL, Alexander TW, Hendrick S, McAllister TA. Characterization of Mannheimia haemolytica isolated from feedlot cattle that were healthy or treated for bovine respiratory disease. Can J Vet Res. 2014;78:38\u201345.","journal-title":"Can J Vet Res"},{"key":"3121_CR6","first-page":"466","volume":"31","author":"L Fodor","year":"1984","unstructured":"Fodor L, Varga J, Hajtos I, Szemeredi GY. Serotypes of Pasteurella haemolytica isolated from sheep, goats and calves. Zoonoses Public Health. 1984;31:466\u20139.","journal-title":"Zoonoses Public Health"},{"key":"3121_CR7","doi-asserted-by":"publisher","first-page":"109121","DOI":"10.1016\/j.vetmic.2021.109121","volume":"258","author":"JL Arnal","year":"2021","unstructured":"Arnal JL, Fern\u00e1ndez A, Vela AI, Sanz C, Fern\u00e1ndez-Garyz\u00e1bal JF. Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain. Vet Microbiol. 2021;258:109121.","journal-title":"Vet Microbiol"},{"key":"3121_CR8","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.mimet.2017.05.010","volume":"139","author":"CL Klima","year":"2007","unstructured":"Klima CL, Zaheer R, Briggs RE, McAllister TA. A multiplex PCR assay for molecular capsular serotyping of Mannheimia haemolytica serotypes 1, 2, and 6. J Microbiol Methods. 2007;139:155\u201360.","journal-title":"J Microbiol Methods"},{"key":"3121_CR9","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.rvsc.2018.12.021","volume":"124","author":"S Andr\u00e9s-Lasheras","year":"2019","unstructured":"Andr\u00e9s-Lasheras S, Zaheer R, Klima C, Sanderson H, Polo RO, Milani MRM, et al. Serotyping and antimicrobial resistance of Mannheimia haemolytica strains from European cattle with bovine respiratory disease. Res in Vet Sci. 2019;124:10\u20132.","journal-title":"Res in Vet Sci"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-021-03121-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-021-03121-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-021-03121-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T19:12:17Z","timestamp":1641237137000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-021-03121-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,3]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["3121"],"URL":"https:\/\/doi.org\/10.1186\/s12917-021-03121-3","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,3]]},"assertion":[{"value":"11 August 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 December 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 January 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This paper refers to testing of samples of <i>M haemolytica<\/i> isolates from cattle pneumonia cases that were previously collected by the Scottish Rural College (SRUC) staff during disease surveillance work, where consent for using the samples for research purposes was given. This study was approved by SRUC\u2019s Animal Ethical Review Body (AWERB).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Author KBS is an employee of MSD Animal Health, the funding body of this study. The other authors declare they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"5"}}