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Although\n                      <jats:italic>T. pyogenes<\/jats:italic>\n                      is the causative agent of the disease, the subsequently invaded\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      will predominate the infection by producing a substantial amount of quorum-sensing (QS)-controlled virulence factors, and co-infection of them usually creates serious difficulties for veterinary treatment. In order to find a potential compound that targets both\n                      <jats:italic>T. pyogenes<\/jats:italic>\n                      and\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      , the antibacterial and anti-virulence capacities of 55 compounds, which have similar core structure to the signal molecules of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      QS system, were tested in this study by performing a series of in vitro screening experiments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified that furazolidone could significantly reduce the cell densities of\n                      <jats:italic>T. pyogenes<\/jats:italic>\n                      in mono-culture or in the co-culture with\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      . Although the growth of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      could also be moderately inhibited by furazolidone, the results of phenotypic identification and transcriptomic analysis further revealed that sub-inhibitory furazolidone had remarkable inhibitory effect on the biofilm production, motility, and QS system of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      . Moreover, furazolidone could efficiently protect\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                      models from\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      infection under both fast-killing and slow-killing conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study reports the antibacterial and anti-virulence abilities of furazolidone on\n                      <jats:italic>T. pyogenes<\/jats:italic>\n                      and\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      , and provides a promising strategy and molecular basis for the development of novel anti-infectious drugs to dealing with forest musk deer purulent disease, or other diseases caused by\n                      <jats:italic>T. pyogenes<\/jats:italic>\n                      and\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      co-infection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-022-03216-5","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T18:03:56Z","timestamp":1648145036000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antibacterial and anti-virulence effects of furazolidone on Trueperella pyogenes and Pseudomonas aeruginosa"],"prefix":"10.1186","volume":"18","author":[{"given":"Qin","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heyue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanghua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balakrishnan","family":"Prithiviraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bisong","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,24]]},"reference":[{"key":"3216_CR1","first-page":"522","volume":"30","author":"KL Zhao","year":"2011","unstructured":"Zhao KL, Li XX, Palahati P, Zhang XY, Zeng B, Yue BS. 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