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As an essential functional amino acid of poultry, arginine plays a critical role in anti-inflammatory and anti-oxidative stress.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In this study, the differential expression genes (DEGs) were screened by transcriptomic techniques, and the DEGs in gene networks of inflammatory response-induced by FAdV-4 in broiler\u2019s liver were analyzed by Kyoto encyclopedia of genes and genomes (KEGG) enrichment. The results showed that the cytokines pathway and JAK\/STAT pathway were significantly enriched, in which the DEGs levels of IL-6, IL-1\u03b2, IFN-\u03b1, JAK and STAT were significantly up-regulated after FAdV-4 infection. It was further verified with real-time fluorescence quantitative polymerase chain reaction (Real-time qPCR) and Western blotting (WB) in vitro and in vivo. The findings demonstrated that FAdV-4 induced inflammatory response and activated JAK2\/STAT3 pathway. Furthermore, we investigated whether arginine could alleviate the liver inflammation induced\u00a0by\u00a0FAdV-4. After treatment with 1.92% arginine level diet to broilers or 300\u00a0\u03bcg\/mL arginine culture medium to LMH cell line with FAdV-4 infection at the same time, we found that the mRNA levels of IL-6, IL-1\u03b2, IFN-\u03b1 and the protein levels of p-JAK2, p-STAT3 were down-regulated, compared with FAdV-4 infection group. Furthermore, we confirmed that the inflammation induced by FAdV-4 was ameliorated by pre-treatment with JAK inhibitor AG490 in LMH cells, and it was further alleviated in LMH cells treatment with AG490 and ARG.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>These above results provide new insight that arginine protects hepatocytes against inflammation induced by FAdV-4 through JAK2\/STAT3 signaling pathway.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-022-03282-9","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T05:02:39Z","timestamp":1652936559000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Arginine regulates inflammation response-induced by Fowl Adenovirus serotype 4 via JAK2\/STAT3 pathway"],"prefix":"10.1186","volume":"18","author":[{"given":"Silin","family":"Xiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiling","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changkang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanxi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"3282_CR1","doi-asserted-by":"publisher","first-page":"49","DOI":"10.3389\/fimmu.2018.00049","volume":"9","author":"R Li","year":"2018","unstructured":"Li R, Li G, Lin J, et al. 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The animal protocol used in this study was approved by the Research Ethics Committee of the College of Animal Science, Fujian Agriculture and Forestry University, China (permit number: PZCASFAFU2016004). The experiments were also carried out in compliance with the ARRIVE guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"189"}}