{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:17:38Z","timestamp":1773926258061,"version":"3.50.1"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan, R.O.C.","doi-asserted-by":"crossref","award":["MOST 107\u20132313-B0005\u2013045"],"award-info":[{"award-number":["MOST 107\u20132313-B0005\u2013045"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Feline injection-site sarcomas (FISSs) are malignant mesenchymal tumors of different histotypes. The pathogenesis of FISS has been correlated with chronic inflammation, resulting in neoplastic transformation. Activation of the Janus kinase-signal transducer and activator of transcription 3 (STAT3) have been demonstrated to play a critical role in tumor development by regulating signaling pathways involved in cell proliferation, survival, metastasis, and angiogenesis in human medicine. To characterize the role of STAT3 in FISS, we first detected STAT3 and phosphorylated STAT3 in formalin-fixed and paraffin-embedded (FFPE) FISS tissues using immunohistochemical staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>STAT3 was detected in 88.9% (40\/45) of FISS cases, and phosphorylated STAT3 was detected in 53.3% (24\/45) of cases. However, the expression levels of both forms of STAT3 were not correlated with tumor grade. To study the role of STAT3 in tumor survival, two primary cells derived from FISSs of two cats exhibiting consistent immunophenotypes with their parental FFPE tissues were established. A dose-dependent inhibitory effect on cell proliferation was observed in both primary FISS cells treated with the STAT3 inhibitor, 5-hydroxy-9,10-dioxo-9,10-dihydroanthracene-1-sulfonamide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The STAT 3 may play an important role in the tumorigenesis of FISS and be a potential molecular therapeutic target for FISS.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-022-03352-y","type":"journal-article","created":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T09:05:04Z","timestamp":1657789504000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Investigating the role of signal transducer and activator of transcription 3 in feline injection site sarcoma"],"prefix":"10.1186","volume":"18","author":[{"given":"Cheng-Hsin","family":"Shih","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen-Chen","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Chiang","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hue-Ying","family":"Chiou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,7,14]]},"reference":[{"issue":"4","key":"3352_CR1","doi-asserted-by":"publisher","first-page":"273","DOI":"10.3109\/07357909909040597","volume":"17","author":"MJ Hendrick","year":"1999","unstructured":"Hendrick MJ. Feline vaccine-associated sarcomas. Cancer Invest. 1999;17(4):273\u20137.","journal-title":"Cancer Invest"},{"issue":"8","key":"3352_CR2","first-page":"968","volume":"199","author":"MJ Hendrick","year":"1991","unstructured":"Hendrick MJ, Goldschmidt MH. Do injection site reactions induce fibrosarcomas in cats? J Am Vet Med Assoc. 1991;199(8):968.","journal-title":"J Am Vet Med Assoc"},{"issue":"2","key":"3352_CR3","doi-asserted-by":"crossref","first-page":"249","DOI":"10.2460\/javma.1997.210.02.249","volume":"210","author":"MJ Coyne","year":"1997","unstructured":"Coyne MJ, Reeves NC, Rosen DK. Estimated prevalence of injection-site sarcomas in cats during 1992. J Am Vet Med Assoc. 1997;210(2):249\u201351.","journal-title":"J Am Vet Med Assoc"},{"issue":"2","key":"3352_CR4","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/S0021-9975(96)80005-3","volume":"114","author":"FD Doddy","year":"1996","unstructured":"Doddy FD, Glickman LT, Glickman NW, Janovitz EB. Feline fibrosarcomas at vaccination sites and non-vaccination sites. J Comp Pathol. 1996;114(2):165\u201374.","journal-title":"J Comp Pathol"},{"issue":"1","key":"3352_CR5","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1177\/030098589403100121","volume":"31","author":"MJ Hendrick","year":"1994","unstructured":"Hendrick MJ, Brooks JJ. Postvaccinal sarcomas in the cat: histology and immunohistochemistry. Vet Pathol. 1994;31(1):126\u20139.","journal-title":"Vet Pathol"},{"issue":"9","key":"3352_CR6","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.2460\/javma.2003.223.1283","volume":"223","author":"PH Kass","year":"2003","unstructured":"Kass PH, Spangler WL, Hendrick MJ, McGill LD, Esplin DG, Lester S, et al. Multicenter case-control study of risk factors associated with development of vaccine-associated sarcomas in cats. J Am Vet Med Assoc. 2003;223(9):1283\u201392.","journal-title":"J Am Vet Med Assoc"},{"issue":"3","key":"3352_CR7","doi-asserted-by":"crossref","first-page":"396","DOI":"10.2460\/javma.1993.203.03.396","volume":"203","author":"PH Kass","year":"1993","unstructured":"Kass PH, Barnes WG Jr, Spangler WL, Chomel BB, Culbertson MR. Epidemiologic evidence for a causal relation between vaccination and fibrosarcoma tumorigenesis in cats. J Am Vet Med Assoc. 1993;203(3):396\u2013405.","journal-title":"J Am Vet Med Assoc"},{"issue":"1","key":"3352_CR8","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/S0195-5616(96)50009-4","volume":"26","author":"DW Macy","year":"1996","unstructured":"Macy DW, Hendrick MJ. The potential role of inflammation in the development of postvaccinal sarcomas in cats. Vet Clin North Am Small Anim Pract. 1996;26(1):103\u20139.","journal-title":"Vet Clin North Am Small Anim Pract"},{"issue":"12","key":"3352_CR9","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.2460\/ajvr.1999.60.12.1513","volume":"60","author":"BL Blagburn","year":"1999","unstructured":"Blagburn BL, Vaughan JL, Butler JM, Parks SC. Dose titration of an injectable formulation of lufenuron in cats experimentally infested with fleas. Am J Vet Res. 1999;60(12):1513\u20135.","journal-title":"Am J Vet Res"},{"issue":"12","key":"3352_CR10","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1016\/j.jfms.2011.07.015","volume":"13","author":"JS Munday","year":"2011","unstructured":"Munday JS, Banyay K, Aberdein D, French AF. Development of an injection site sarcoma shortly after meloxicam injection in an unvaccinated cat. J Feline Med Surg. 2011;13(12):988\u201391.","journal-title":"J Feline Med Surg"},{"issue":"10","key":"3352_CR11","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1177\/1098612X12449407","volume":"14","author":"M Martano","year":"2012","unstructured":"Martano M, Morello E, Iussich S, Buracco P. A case of feline injection-site sarcoma at the site of cisplatin injections. J Feline Med Surg. 2012;14(10):751\u20134.","journal-title":"J Feline Med Surg"},{"issue":"2","key":"3352_CR12","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.jfms.2007.10.011","volume":"10","author":"MK Daly","year":"2008","unstructured":"Daly MK, Saba CF, Crochik SS, Howerth EW, Kosarek CE, Cornell KK, et al. Fibrosarcoma adjacent to the site of microchip implantation in a cat. J Feline Med Surg. 2008;10(2):202\u20135.","journal-title":"J Feline Med Surg"},{"issue":"4","key":"3352_CR13","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1158\/1541-7786.MCR-05-0261","volume":"4","author":"H Lu","year":"2006","unstructured":"Lu H, Ouyang W, Huang C. Inflammation, a key event in cancer development. Mol Cancer Res. 2006;4(4):221\u201333.","journal-title":"Mol Cancer Res"},{"issue":"6","key":"3352_CR14","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1354\/vp.40-6-651","volume":"40","author":"A Nieto","year":"2003","unstructured":"Nieto A, Sanchez MA, Martinez E, Rollan E. Immunohistochemical expression of p53, fibroblast growth factor-b, and transforming growth factor-alpha in feline vaccine-associated sarcomas. Vet Pathol. 2003;40(6):651\u20138.","journal-title":"Vet Pathol"},{"issue":"1","key":"3352_CR15","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1177\/030098589002700111","volume":"27","author":"RR Dubielzig","year":"1990","unstructured":"Dubielzig RR, Everitt J, Shadduck JA, Albert DM. Clinical and morphologic features of post-traumatic ocular sarcomas in cats. Vet Pathol. 1990;27(1):62\u20135.","journal-title":"Vet Pathol"},{"issue":"7203","key":"3352_CR16","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1038\/nature07205","volume":"454","author":"A Mantovani","year":"2008","unstructured":"Mantovani A, Allavena P, Sica A, Balkwill F. Cancer-related inflammation. Nature. 2008;454(7203):436\u201344.","journal-title":"Nature"},{"key":"3352_CR17","first-page":"98","volume":"2","author":"G Multhoff","year":"2011","unstructured":"Multhoff G, Molls M, Radons J. Chronic inflammation in cancer development. Front Immunol. 2011;2:98.","journal-title":"Front Immunol"},{"issue":"3","key":"3352_CR18","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.cell.2004.07.013","volume":"118","author":"FR Greten","year":"2004","unstructured":"Greten FR, Eckmann L, Greten TF, Park JM, Li ZW, Egan LJ, et al. IKKbeta links inflammation and tumorigenesis in a mouse model of colitis-associated cancer. Cell. 2004;118(3):285\u201396.","journal-title":"Cell"},{"issue":"7007","key":"3352_CR19","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/nature02924","volume":"431","author":"E Pikarsky","year":"2004","unstructured":"Pikarsky E, Porat RM, Stein I, Abramovitch R, Amit S, Kasem S, et al. NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature. 2004;431(7007):461\u20136.","journal-title":"Nature"},{"issue":"1","key":"3352_CR20","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1186\/s12917-019-2100-9","volume":"15","author":"CS Hsueh","year":"2019","unstructured":"Hsueh CS, Wu CH, Shih CH, Yeh JL, Jeng CR, Pang VF, et al. Role of nuclear factor-kappa B in feline injection site sarcoma. BMC Vet Res. 2019;15(1):365.","journal-title":"BMC Vet Res"},{"issue":"7","key":"3352_CR21","doi-asserted-by":"publisher","first-page":"1877","DOI":"10.1038\/jid.2012.45","volume":"132","author":"M Sakaguchi","year":"2012","unstructured":"Sakaguchi M, Oka M, Iwasaki T, Fukami Y, Nishigori C. Role and regulation of STAT3 phosphorylation at Ser727 in melanocytes and melanoma cells. J Invest Dermatol. 2012;132(7):1877\u201385.","journal-title":"J Invest Dermatol"},{"issue":"1","key":"3352_CR22","volume":"2","author":"R Fagard","year":"2013","unstructured":"Fagard R, Metelev V, Souissi I, Baran-Marszak F. STAT3 inhibitors for cancer therapy: Have all roads been explored? Jakstat. 2013;2(1):e22882.","journal-title":"Jakstat"},{"issue":"2","key":"3352_CR23","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1038\/s41568-018-0090-8","volume":"19","author":"J Huynh","year":"2019","unstructured":"Huynh J, Chand A, Gough D, Ernst M. Therapeutically exploiting STAT3 activity in cancer - using tissue repair as a road map. Nat Rev Cancer. 2019;19(2):82\u201396.","journal-title":"Nat Rev Cancer"},{"issue":"6","key":"3352_CR24","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1038\/nri818","volume":"2","author":"WS Alexander","year":"2002","unstructured":"Alexander WS. Suppressors of cytokine signalling (SOCS) in the immune system. Nat Rev Immunol. 2002;2(6):410\u20136.","journal-title":"Nat Rev Immunol"},{"issue":"8","key":"3352_CR25","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1038\/nri1667","volume":"5","author":"K Shuai","year":"2005","unstructured":"Shuai K, Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat Rev Immunol. 2005;5(8):593\u2013605.","journal-title":"Nat Rev Immunol"},{"issue":"4","key":"3352_CR26","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1177\/0300985814559400","volume":"52","author":"K Hughes","year":"2015","unstructured":"Hughes K, Scase TJ, Foote AK. Estrogen Receptor and Signal Transducer and Activator of Transcription 3 Expression in Equine Mammary Tumors. Vet Pathol. 2015;52(4):631\u20134.","journal-title":"Vet Pathol"},{"issue":"6","key":"3352_CR27","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1007\/s11259-006-3335-z","volume":"30","author":"C Petterino","year":"2006","unstructured":"Petterino C, Ratto A, Arcicasa E, Podesta G, Drigo M, Pellegrino C. Expression of Stat3 in feline mammary gland tumours and its relation to histological grade. Vet Res Commun. 2006;30(6):599\u2013611.","journal-title":"Vet Res Commun"},{"issue":"2","key":"3352_CR28","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1177\/1040638717745575","volume":"30","author":"K Hughes","year":"2018","unstructured":"Hughes K, Scott VHL, Blanck M, Barnett TP, Spanner Kristiansen J, Foote AK. Equine renal hemangiosarcoma: clinical presentation, pathologic features, and pSTAT3 expression. J Vet Diagn Invest. 2018;30(2):268\u201374.","journal-title":"J Vet Diagn Invest"},{"key":"3352_CR29","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1186\/s12917-015-0505-7","volume":"11","author":"ME Brown","year":"2015","unstructured":"Brown ME, Bear MD, Rosol TJ, Premanandan C, Kisseberth WC, London CA. Characterization of STAT3 expression, signaling and inhibition in feline oral squamous cell carcinoma. BMC Vet Res. 2015;11:206.","journal-title":"BMC Vet Res"},{"issue":"1","key":"3352_CR30","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1111\/jvim.14860","volume":"32","author":"A Assumpcao","year":"2018","unstructured":"Assumpcao A, Jark PC, Hong CC, Lu Z, Ruetten HM, Heaton CM, et al. STAT3 Expression and Activity are Up-Regulated in Diffuse Large B Cell Lymphoma of Dogs. J Vet Intern Med. 2018;32(1):361\u20139.","journal-title":"J Vet Intern Med"},{"issue":"1","key":"3352_CR31","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.jcpa.2005.07.005","volume":"134","author":"C Petterino","year":"2006","unstructured":"Petterino C, Martano M, Cascio P, Cerruti F, Martini M, Morello E, et al. Immunohistochemical study of STAT3 expression in feline injection-site fibrosarcomas. J Comp Pathol. 2006;134(1):91\u2013100.","journal-title":"J Comp Pathol"},{"issue":"2","key":"3352_CR32","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/s11259-006-3330-4","volume":"31","author":"C Petterino","year":"2007","unstructured":"Petterino C, Podest\u00e0 G, Ratto A, Drigo M, Pellegrino C. Immunohistochemical study of phospho-Stat3-ser727 expression in feline mammary gland tumours. Vet Res Commun. 2007;31(2):173\u201384.","journal-title":"Vet Res Commun"},{"issue":"6","key":"3352_CR33","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1136\/jcp.2006.036970","volume":"60","author":"SF Yang","year":"2007","unstructured":"Yang SF, Wang SN, Wu CF, Yeh YT, Chai CY, Chunag SC, et al. Altered p-STAT3 (tyr705) expression is associated with histological grading and intratumour microvessel density in hepatocellular carcinoma. J Clin Pathol. 2007;60(6):642\u20138.","journal-title":"J Clin Pathol"},{"issue":"5","key":"3352_CR34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127356","volume":"10","author":"MX Li","year":"2015","unstructured":"Li MX, Bi XY, Huang Z, Zhao JJ, Han Y, Li ZY, et al. Prognostic Role of Phospho-STAT3 in Patients with Cancers of the Digestive System: A Systematic Review and Meta-Analysis. PLoS One. 2015;10(5):e0127356.","journal-title":"PLoS One"},{"issue":"4","key":"3352_CR35","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1002\/hed.21468","volume":"33","author":"MA Macha","year":"2011","unstructured":"Macha MA, Matta A, Kaur J, Chauhan SS, Thakar A, Shukla NK, et al. Prognostic significance of nuclear pSTAT3 in oral cancer. Head Neck. 2011;33(4):482\u20139.","journal-title":"Head Neck"},{"issue":"1","key":"3352_CR36","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1177\/0300985810388820","volume":"48","author":"MM Dennis","year":"2011","unstructured":"Dennis MM, McSporran KD, Bacon NJ, Schulman FY, Foster RA, Powers BE. Prognostic factors for cutaneous and subcutaneous soft tissue sarcomas in dogs. Vet Pathol. 2011;48(1):73\u201384.","journal-title":"Vet Pathol"},{"issue":"1","key":"3352_CR37","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/s00418-018-1742-1","volume":"151","author":"S Libard","year":"2019","unstructured":"Libard S, Cerjan D, Alafuzoff I. Characteristics of the tissue section that influence the staining outcome in immunohistochemistry. Histochem Cell Biol. 2019;151(1):91\u20136.","journal-title":"Histochem Cell Biol"},{"issue":"1","key":"3352_CR38","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.cytogfr.2009.11.005","volume":"21","author":"SI Grivennikov","year":"2010","unstructured":"Grivennikov SI, Karin M. Dangerous liaisons: STAT3 and NF-kappaB collaboration and crosstalk in cancer. Cytokine Growth Factor Rev. 2010;21(1):11\u20139.","journal-title":"Cytokine Growth Factor Rev"},{"issue":"11","key":"3352_CR39","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/nrc3818","volume":"14","author":"H Yu","year":"2014","unstructured":"Yu H, Lee H, Herrmann A, Buettner R, Jove R. Revisiting STAT3 signalling in cancer: new and unexpected biological functions. Nat Rev Cancer. 2014;14(11):736\u201346.","journal-title":"Nat Rev Cancer"},{"issue":"23","key":"3352_CR40","doi-asserted-by":"publisher","first-page":"8150","DOI":"10.1073\/pnas.0501643102","volume":"102","author":"L Liu","year":"2005","unstructured":"Liu L, McBride KM, Reich NC. STAT3 nuclear import is independent of tyrosine phosphorylation and mediated by importin-alpha3. Proc Natl Acad Sci U S A. 2005;102(23):8150\u20135.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"3352_CR41","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1159\/000448524","volume":"84","author":"H Bekki","year":"2017","unstructured":"Bekki H, Kohashi K, Yamada Y, Iura K, Ishii T, Maekawa A, et al. Phosphorylation of STAT3 in Undifferentiated Pleomorphic Sarcoma Is Correlated with a Favorable Prognosis. Pathobiology. 2017;84(3):161\u20139.","journal-title":"Pathobiology"},{"issue":"2","key":"3352_CR42","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1177\/0300985814543198","volume":"52","author":"SW Mills","year":"2015","unstructured":"Mills SW, Musil KM, Davies JL, Hendrick S, Duncan C, Jackson ML, et al. Prognostic value of histologic grading for feline mammary carcinoma: a retrospective survival analysis. Vet Pathol. 2015;52(2):238\u201349.","journal-title":"Vet Pathol"},{"issue":"4","key":"3352_CR43","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1177\/1098612X18774709","volume":"21","author":"CS Carneiro","year":"2019","unstructured":"Carneiro CS, de Queiroz GF, Pinto AC, Dagli ML, Matera JM. Feline injection site sarcoma: immunohistochemical characteristics. J Feline Med Surg. 2019;21(4):314\u201321.","journal-title":"J Feline Med Surg"},{"issue":"1","key":"3352_CR44","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.tvjl.2009.07.019","volume":"186","author":"C Giudice","year":"2010","unstructured":"Giudice C, Stefanello D, Sala M, Cantatore M, Russo F, Romussi S, et al. Feline injection-site sarcoma: recurrence, tumour grading and surgical margin status evaluated using the three-dimensional histological technique. Vet J. 2010;186(1):84\u20138.","journal-title":"Vet J"},{"issue":"2","key":"3352_CR45","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1177\/0300985816677148","volume":"54","author":"I Porcellato","year":"2017","unstructured":"Porcellato I, Menchetti L, Brachelente C, Sforna M, Reginato A, Lepri E, et al. Feline Injection-Site Sarcoma. Vet Pathol. 2017;54(2):204\u201311.","journal-title":"Vet Pathol"},{"issue":"8","key":"3352_CR46","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.5858\/arpa.2016-0417-RA","volume":"141","author":"S Wei","year":"2017","unstructured":"Wei S, Henderson-Jackson E, Qian X, Bui MM. Soft Tissue Tumor Immunohistochemistry Update: Illustrative Examples of Diagnostic Pearls to Avoid Pitfalls. Arch Pathol Lab Med. 2017;141(8):1072\u201391.","journal-title":"Arch Pathol Lab Med"},{"issue":"1","key":"3352_CR47","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/nri1995","volume":"7","author":"H Yu","year":"2007","unstructured":"Yu H, Kortylewski M, Pardoll D. Crosstalk between cancer and immune cells: role of STAT3 in the tumour microenvironment. Nat Rev Immunol. 2007;7(1):41\u201351.","journal-title":"Nat Rev Immunol"},{"issue":"1","key":"3352_CR48","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/cr.2010.183","volume":"21","author":"G He","year":"2011","unstructured":"He G, Karin M. NF-kappaB and STAT3 - key players in liver inflammation and cancer. Cell Res. 2011;21(1):159\u201368.","journal-title":"Cell Res"},{"issue":"1","key":"3352_CR49","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1593\/neo.91196","volume":"12","author":"L Lin","year":"2010","unstructured":"Lin L, Hutzen B, Li PK, Ball S, Zuo M, DeAngelis S, et al. A novel small molecule, LLL12, inhibits STAT3 phosphorylation and activities and exhibits potent growth-suppressive activity in human cancer cells. Neoplasia. 2010;12(1):39\u201350.","journal-title":"Neoplasia"},{"key":"3352_CR50","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1186\/1746-6148-8-244","volume":"8","author":"JI Couto","year":"2012","unstructured":"Couto JI, Bear MD, Lin J, Pennel M, Kulp SK, Kisseberth WC, et al. Biologic activity of the novel small molecule STAT3 inhibitor LLL12 against canine osteosarcoma cell lines. BMC Vet Res. 2012;8:244.","journal-title":"BMC Vet Res"},{"issue":"8","key":"3352_CR51","doi-asserted-by":"publisher","first-page":"1229","DOI":"10.1016\/j.yexcr.2005.12.011","volume":"312","author":"P Fang","year":"2006","unstructured":"Fang P, Hwa V, Rosenfeld RG. Interferon-gamma-induced dephosphorylation of STAT3 and apoptosis are dependent on the mTOR pathway. Exp Cell Res. 2006;312(8):1229\u201339.","journal-title":"Exp Cell Res"},{"issue":"3","key":"3352_CR52","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s00418-008-0474-z","volume":"130","author":"JW Mandell","year":"2008","unstructured":"Mandell JW. Immunohistochemical assessment of protein phosphorylation state: the dream and the reality. Histochem Cell Biol. 2008;130(3):465\u201371.","journal-title":"Histochem Cell Biol"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-022-03352-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-022-03352-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-022-03352-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T03:47:26Z","timestamp":1727581646000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-022-03352-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,14]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["3352"],"URL":"https:\/\/doi.org\/10.1186\/s12917-022-03352-y","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,14]]},"assertion":[{"value":"6 January 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 June 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All procedures involving animal sample collection were performed in accordance with guidelines of ARRIVE () and Institutional Animal Care and Use Committee of National Taiwan University (NTU; Taiwan, Republic of China) and National Chung Hsing University (NCHU) and carried out under the regulation and permission of the IACUC protocol (NTU107-EL- 00165) at NTU and (107-063) at NCHU, respectively.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"<!--Emphasis Type='Bold' removed-->Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"<!--Emphasis Type='Bold' removed-->Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"276"}}