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In this study, we characterized the dynamics of<jats:italic>T. cati<\/jats:italic>larvae migration in BALB\/c mice after inoculation with eggs and ensured the migration detecting the larval DNA by a PCR. To evaluate the dynamics of larval migration and distribution, twenty-four BALB\/c mice were orally inoculated with 2500\u00a0T<jats:italic>. cati<\/jats:italic>infective eggs and the visceral organs of the infected animals were examined by pepsin digestion and microscopic parasite counts, followed by PCR at day 1 to 28 post-inoculation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PCR assays were successfully used for detection of<jats:italic>T. cati<\/jats:italic>larvae in tissue samples and<jats:italic>T. cati<\/jats:italic>larvae and the DNAs were found in the liver, lungs, heart, kidneys and the brain. We detected<jats:italic>T. cati<\/jats:italic>in 92.2% of tissue samples by PCR, 30% higher than the conventional pepsin digestion technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings demonstrated that the PCR assay is a sensitive and specific for the detection of<jats:italic>T. cati<\/jats:italic>larvae. Therefore, it could become a useful tool for the investigation of the dynamics of larval migration and<jats:italic>Toxocara<\/jats:italic>infection in murine model.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12917-022-03366-6","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T06:02:54Z","timestamp":1657000974000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Larva migrans in BALB\/c mice experimentally infected with Toxocara cati ensured by PCR assay"],"prefix":"10.1186","volume":"18","author":[{"given":"Majid","family":"Naderbandi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2265-7460","authenticated-orcid":false,"given":"Mohammad","family":"Zibaei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3200-3981","authenticated-orcid":false,"given":"Ali","family":"Haniloo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzaneh","family":"Firoozeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Hatami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elham","family":"Shokri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensuke","family":"Taira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,7,5]]},"reference":[{"key":"3366_CR1","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.ijppaw.2020.02.011","volume":"11","author":"AF French","year":"2020","unstructured":"French AF, Castillo-Alcala F, Gedye KR, Roe WD, Gartrell BD. 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Informed consent was obtained from the owner or legal custodian of all animals described in this study for the procedures undertaken. The ethical statement of this study was approved and written by the Ethical Committee for Zanjan University of Medical Sciences with the code of IR.ZUMS.REC.1400.133. All methods were carried out in accordance with relevant guidelines and regulations. This project was partially supported by the Vice Chancellor for Research of Zanjan University of Medical Sciences by the code A-10\u201395-14.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"257"}}