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In recent years, the increasing prevalence of antimicrobial resistant <jats:italic>Salmonella<\/jats:italic> poses a threat to global public health. The purpose of this study is to the prevalence of antimicrobial resistant <jats:italic>Salmonella<\/jats:italic> in pig slaughterhouses in Hubei Province in China, and explore the effect of using lytic bacteriophages fighting against antimicrobial resistant <jats:italic>Salmonella<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We collected a total of 1289 samples including anal swabs of pigs (862\/1289), environmental swabs (204\/1289), carcass surface swabs (36\/1289) and environmental agar plates (187\/1289) from eleven slaughterhouses in seven cities in Hubei Province and recovered 106 <jats:italic>Salmonella<\/jats:italic> isolates. Antimicrobial susceptibility testing revealed that these isolates showed a high rate of antimicrobial resistance; over 99.06% (105\/106) of them were multidrug resistant. To combat these drug resistant <jats:italic>Salmonella<\/jats:italic>, we isolated 37 lytic phages using 106 isolates as indicator bacteria. One of them, designated ph\u20092\u20132, which belonged to the <jats:italic>Myoviridae<\/jats:italic> family, displayed good capacity to kill <jats:italic>Salmonella<\/jats:italic> under different adverse conditions (exposure to different temperatures, pHs, UV, and\/or 75% ethanol) and had a wide lytic spectrum. Evaluation in mouse models showed that ph\u20092\u20132 was safe and saved 80% (administrated by gavage) and 100% (administrated through intraperitoneal injection) mice from infections caused by <jats:italic>Salmonella Typhimurium<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The data presented herein demonstrated that <jats:italic>Salmonella<\/jats:italic> contamination remains a problem in some pig slaughter houses in China and <jats:italic>Salmonella<\/jats:italic> isolates recovered in slaughter houses displayed a high rate of antimicrobial resistance. In addition, broad-spectrum lytic bacteriophages may represent a good candidate for the development of anti-antimicrobial resistant <jats:italic>Salmonella<\/jats:italic> agents.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-022-03372-8","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T19:03:19Z","timestamp":1657652599000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of a broad-spectrum lytic Myoviridae bacteriophage using multidrug resistant Salmonella isolates from pig slaughterhouses as the indicator and its application in combating Salmonella infections"],"prefix":"10.1186","volume":"18","author":[{"given":"Mengfei","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangru","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanchun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5249-328X","authenticated-orcid":false,"given":"Zhong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"3372_CR1","unstructured":"CDC: Salmonella. https:\/\/www.cdc.gov\/salmonella\/. 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