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In order to elucidate the pathogenic mechanism and innate immune response of <jats:italic>P. multocida<\/jats:italic>, an infection experiment was carried out in this study.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Our results showed that the clinical symptoms of rabbits were severe dyspnoea and serous nasal fluid. During the course of the disease, the deaths peaked at 2\u2009days post infection (dpi) and mortality rate was 60%. The pathological changes of the lung, trachea, and thymus were observed. In particular, consolidation and abscesses appeared in lung. Histopathologic changes in rabbits showed edema, hemorrhage, and neutrophil infiltration in the lung. <jats:italic>P. multocida<\/jats:italic> can rapidly replicate in a variety of tissues, and the colonization in most of the tested tissues reached the maximum at 2 dpi and then decreased at 3 dpi. The number of <jats:italic>P. multocida<\/jats:italic> in lung and thymus remained high level at 3 dpi. Toll-like receptors 2 and 4 signaling pathways were activated after <jats:italic>P. multocida<\/jats:italic> infection. The expression of <jats:italic>Il1\u03b2<\/jats:italic>, <jats:italic>Il6<\/jats:italic>, <jats:italic>Il8<\/jats:italic>, and <jats:italic>Tnf-\u03b1<\/jats:italic> was significantly increased. The expression of most proinflammatory cytokines peaked at 2 dpi and decreased at 3 dpi, and the expression trend of cytokines was consistent with the colonization of <jats:italic>P. multocida<\/jats:italic> in rabbit tissues.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The <jats:italic>P. multocida<\/jats:italic> can rapidly replicate in various tissues of rabbit and cause bacteremia after infection. TLRs signaling pathways were activated after <jats:italic>P. multocida<\/jats:italic> infection, significantly inducing the expression of proinflammatory cytokines, which is might the main cause of respiratory inflammation and septicemia.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-022-03517-9","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T09:04:28Z","timestamp":1669712668000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Pathogenicity, colonization, and innate immune response to Pasteurella multocida in rabbits"],"prefix":"10.1186","volume":"18","author":[{"given":"Wenhao","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingtao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjiao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yantao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,26]]},"reference":[{"issue":"3","key":"3517_CR1","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1128\/CMR.00024-13","volume":"26","author":"BA Wilson","year":"2013","unstructured":"Wilson BA, Ho M. 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All experiments were approved by the Committee on the Ethics of Animal Experiments of Yangzhou University (202203180). This study was reported in accordance with the ARRIVE guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"416"}}