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At present, commercial inactivated BVDV vaccines may cause some adverse reactions during use. This study aims to develop a safer and more efficient inactivated BVDV vaccine.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Here, we described the generation and preclinical efficacy of a hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) inactivated BVDV type 1 vaccine in mice, and administered it separately with commercial vaccine (formaldehyde inactivated) in mice to study its efficacy.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The BVDV type 1 IgG, IFN- \u03b3, IL-4 and neutralizing antibody in the serum of the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> inactivated vaccine group can be maintained in mice for 70\u00a0days. The IgG level reached its maximum value of 0.67 on the 42nd day, significantly higher than the commercial formaldehyde inactivated BVDV type 1 vaccine. IFN- \u03b3 and IL-4 reached their maximum values on the 28th day after immunization, at 123.16\u00a0pg\/ml and 143.80\u00a0pg\/ml, respectively, slightly higher than commercial vaccines, but the effect was not significant. At the same time the BVDV\u20141 neutralizing antibody titer reached a maximum of 12 Nu on the 42nd day post vaccination.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> inactivated BVDV vaccine has good safety and immunogenicity, which provides a potential solution for the further development of an efficient and safe BVDV vaccine.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-03897-0","type":"journal-article","created":{"date-parts":[[2024,2,3]],"date-time":"2024-02-03T02:03:43Z","timestamp":1706925823000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Efficacy of H2O2 inactivated bovine virus diarrhoea virus (BVDV) type 1 vaccine in mice"],"prefix":"10.1186","volume":"20","author":[{"given":"Cunyuan","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinming","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxuan","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiping","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengwei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,2,3]]},"reference":[{"key":"3897_CR1","doi-asserted-by":"publisher","first-page":"114","DOI":"10.4149\/av_2014_02_114","volume":"58","author":"V Gupta","year":"2014","unstructured":"Gupta V, Mishra N, Pateriya A, Behera SP, Rajukumar K. 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All procedures were carried out in accordance with relevant guidelines (e.g., ARRIVE) and regulations when applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"43"}}