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At present, there is still no effective commercial vaccine against PmA infection. In this study, a mutant strain (PmCQ2\u03944555\u20134580) with brand-new phenotypes was obtained after serially passaging at 42\u00a0\u00b0C. Whole genome resequencing and PCR analysis showed that PmCQ2\u03944555\u20134580 missed six genes, including PmCQ2_004555, PmCQ2_004560, PmCQ2_004565, PmCQ2_004570, PmCQ2_004575, and PmCQ2_004580. Importantly, the virulence of PmCQ2\u03944555\u20134580 was reduced by approximately 2.8\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> times in mice. Notably, live PmCQ2\u03944555\u20134580 could provide 100%, 100% and 40% protection against PmA, PmB and PmF, respectively; and inactivated PmCQ2\u03944555\u20134580 could provide 100% and 87.5% protection against PmA and PmB. Interestingly, immune protection-related proteins were significantly upregulated in PmCQ2\u03944555\u20134580 based on RNA-seq and bioinformatics analysis. Meaningfully, by in vitro expression, purification and in vivo immunization, 12 proteins had different degrees of immune protective effects. Among them, PmCQ2_008205, PmCQ2_010435, PmCQ2_008190, and PmCQ2_004170 had the best protective effect, the protection rates against PmA were 50%, 40%, 30%, and 30%, respectively, and the protective rates against PmB were 62.5%, 42.9%, 37.5%, and 28.6%, respectively. Collectively, PmCQ2\u03944555\u20134580 is a potential vaccine candidate for the prevention of Pasteurellosis involving in high expression of immune protective related proteins.<\/jats:p>","DOI":"10.1186\/s12917-024-03948-6","type":"journal-article","created":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T06:01:45Z","timestamp":1709964105000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Attenuated vaccine PmCQ2\u03944555\u20134580 effectively protects mice against Pasteurella multocida infection"],"prefix":"10.1186","volume":"20","author":[{"given":"Fang","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangfu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nengzhang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyi","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,9]]},"reference":[{"issue":"2","key":"3948_CR1","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1136\/vr.121.2.44","volume":"121","author":"MC De Alwis","year":"1987","unstructured":"De Alwis MC. 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