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The development of novel vaccines for <jats:italic>SE<\/jats:italic> is necessary to eradicate this pathogen. Genetically engineered attenuated live vaccines are more immunogenic and safer. Thus, to develop a live attenuated <jats:italic>Salmonella<\/jats:italic> vaccine, we constructed a <jats:italic>cheV<\/jats:italic> gene deletion strain of <jats:italic>SE<\/jats:italic> (named \u0394<jats:italic>cheV<\/jats:italic>) and investigated the role of <jats:italic>cheV<\/jats:italic> in the virulence of <jats:italic>SE<\/jats:italic>. First, the ability to resist environmental stress in vitro, biofilm formation capacity, drug resistance and motility of \u0394<jats:italic>cheV<\/jats:italic> were analyzed. Secondly, the bacterial adhesion, invasion, intracellular survival assays were performed by cell model. Using a mouse infection model, an in vivo virulence assessment was conducted. To further evaluate the mechanisms implicated by the reduced virulence, qPCR analysis was utilized to examine the expression of the strain\u2019s major virulence genes. Finally, the immune protection rate of \u0394<jats:italic>cheV<\/jats:italic> was evaluated using a mouse model.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Compared to C50336, the \u0394<jats:italic>cheV<\/jats:italic> had significantly reduced survival ability under acidic, alkaline and thermal stress conditions, but there was no significant difference in survival under oxidative stress conditions. There was also no significant change in biofilm formation ability, drug resistance and motility. It was found that the adhesion ability of \u0394<jats:italic>cheV<\/jats:italic> to Caco-2 cells remained unchanged, but the invasion ability and survival rate in RAW264.7 cells were significantly reduced. The challenge assay results showed that the LD<jats:sub>50<\/jats:sub> values of C50336 and \u0394<jats:italic>cheV<\/jats:italic> were 6.3\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> CFU and 1.25\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> CFU, respectively. After the deletion of the <jats:italic>cheV<\/jats:italic> gene, the expression levels of <jats:italic>fimD<\/jats:italic>, <jats:italic>flgG<\/jats:italic>, <jats:italic>csgA<\/jats:italic>, <jats:italic>csgD<\/jats:italic>, <jats:italic>hflK<\/jats:italic>, <jats:italic>lrp<\/jats:italic>, <jats:italic>sipA<\/jats:italic>, <jats:italic>sipB<\/jats:italic>, <jats:italic>pipB<\/jats:italic>, <jats:italic>invH<\/jats:italic>, <jats:italic>mgtC<\/jats:italic>, <jats:italic>sodC<\/jats:italic>, <jats:italic>rfbH<\/jats:italic>, <jats:italic>xthA<\/jats:italic> and <jats:italic>mrr1<\/jats:italic> genes were significantly reduced. The live attenuated \u0394<jats:italic>cheV<\/jats:italic> provided 100% protection in mice against <jats:italic>SE<\/jats:italic> infection.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>All the results confirmed that the deletion of the <jats:italic>cheV<\/jats:italic> gene reduces the virulence of <jats:italic>SE<\/jats:italic> and provides significant immune protection in mice, indicating that \u0394<jats:italic>cheV<\/jats:italic> could be potential candidates to be explored as live-attenuated vaccines.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-03951-x","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T08:01:46Z","timestamp":1710144106000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["CheV enhances the virulence of Salmonella Enteritidis, and the Chev-deleted Salmonella vaccine provides immunity in mice"],"prefix":"10.1186","volume":"20","author":[{"given":"Lu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonglei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guixin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiumei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"issue":"14","key":"3951_CR1","doi-asserted-by":"publisher","first-page":"e00591","DOI":"10.1128\/AEM.00591-19","volume":"85","author":"RG Ferrari","year":"2019","unstructured":"Ferrari RG, Rosario DKA, Cunha-Neto A, Mano SB, Figueiredo EES, Conte-Junior CA. 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