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Physicochemical parameters of water were analyzed, and heavy metal levels were measured. The fish samples were subjected to clinical, bacteriological, Enterobacterial Repetitive Intergenic Consensus (ERIC) fingerprinting, and hematoxylin and Eosin histopathological staining.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The results revealed significant variations in the water quality parameters over different seasons, in addition to an increase in heavy metals. Naturally infected fish showed external signs and postmortem lesions that were relevant to bacterial infection. Two dominant <jats:italic>Vibrio<\/jats:italic> subspecies of bacteria were identified: <jats:italic>V. alginolyticus<\/jats:italic> (205 isolates) and <jats:italic>V. fluvialis<\/jats:italic> (87 isolates). PCR confirmed <jats:italic>the presence of V. alginolyticus<\/jats:italic> using the species-specific primer collagenase at 737 bp. The highest prevalence of <jats:italic>V. alginolyticus<\/jats:italic> was detected during the summer season (57.72%), and the lowest prevalence was observed in autumn (39.75%). The correlation analysis revealed a positive relationship between <jats:italic>V. alginolyticus<\/jats:italic> and water temperature (<jats:italic>r<\/jats:italic>\u2009=\u20090.69). On the other hand, <jats:italic>V. fluvialis<\/jats:italic> showed a high prevalence during the autumn season (25.30%) and the lowest prevalence during the summer season (10.56%), where it was negatively correlated with water temperatures (<jats:italic>r<\/jats:italic>\u2009=\u20140.03). ERIC fingerprinting showed genetic variation within the <jats:italic>Vibrio<\/jats:italic> isolates. Antimicrobial susceptibility testing revealed sensitivity to ciprofloxacin and doxycycline, and resistance to amoxicillin and erythromycin. The multiple antibiotic resistance (MAR) index values for <jats:italic>V. alginolyticus<\/jats:italic> and <jats:italic>V. fluvialis<\/jats:italic> ranged from 0.3 to 0.7, with a multi-drug resistance pattern to at least three antibiotics. Histopathological alterations in the affected tissues revealed marked hemorrhage, vascular congestion, and hemosiderosis infiltration.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study provides insights into the potential propagation of waterborne diseases and antibiotic resistance in the environment. Ensuring that the environment does not serve as a reservoir for virulent and contagious <jats:italic>Vibrio<\/jats:italic> species is a critical concern for regional aquaculture industries. Therefore, we recommend implementing environmental context-specific monitoring and surveillance tools for microbial resistance.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-03978-0","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T12:02:16Z","timestamp":1711972936000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prevalence, genetic diversity, and antimicrobial susceptibility of Vibrio spp. infected gilthead sea breams from coastal farms at Damietta, Egypt"],"prefix":"10.1186","volume":"20","author":[{"given":"Esraa Tawfeek","family":"Ismail","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai A. 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