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In contrast to human patients with heart failure (HF), iron deficiency (ID) prevalence in dogs with MMVD is weakly known. The study aimed to assess the usability of ID markers in serum and reticulocyte parameters from whole blood of dogs with MMVD to evaluate early ID symptoms.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Sixty-eight dogs (43 male and 25 female) were included in the study. MMVD dogs were assigned according to the 2019 ACVIM guidelines for groups B1 (<jats:italic>n<\/jats:italic>\u2009=\u20099), B2 (<jats:italic>n<\/jats:italic>\u2009=\u200910), C (<jats:italic>n<\/jats:italic>\u2009=\u200927) and D (<jats:italic>n<\/jats:italic>\u2009=\u200910). Groups were also combined into B1 and B2 as non-symptomatic HF and C with D as symptomatic HF. Healthy controls were 12 dogs. Serum iron concentration below the reference range in dogs with MMVD was 12.5%. Other ID indices, such as %SAT, UIBC, and TIBC were similar in the MMVD groups and healthy controls (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05 for all parameters). Statistical comparison between control group and 4 groups of different stages of MMVD showed that significant differences occur only in serum transferrin. The assessment of ferritin and soluble transferrin receptors using Western Blotting did not show differences between control (<jats:italic>n<\/jats:italic>\u2009=\u20097) and MMVD (<jats:italic>n<\/jats:italic>\u2009=\u200933) dogs. Study has shown positive correlation between ID parameters and echocardiographic indices such as LA\/Ao and LVIDdN, and some biochemical parameters. A significant increase in reticulocytes percentage, assessed manually, was observed in the HF group of animals (<jats:italic>p<\/jats:italic>\u2009=\u20090.027) compared to the control group.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Studies have shown that ID parameters in serum are not significantly different in dogs with MMVD compared to healthy dogs. However, there is a clear correlation between atrial size and normalised left ventricular size to body size and some biochemical parameters, including ID parameters and therefore the severity of MMVD.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-04071-2","type":"journal-article","created":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T04:01:46Z","timestamp":1716004906000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Iron parameters analysis in dogs with myxomatous mitral valve disease"],"prefix":"10.1186","volume":"20","author":[{"given":"Ewa","family":"Kumiega","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil","family":"A Kobak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Noszczyk-Nowak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Kasztura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,18]]},"reference":[{"key":"4071_CR1","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.cvsm.2010.03.008","volume":"40","author":"M Borgarelli","year":"2010","unstructured":"Borgarelli M, Haggstrom J. 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Animal Welfare Advisory Team of Faculty of Veterinary Medicine accepted research (approval no. 13\/2022). Ethical review and approval from the Local Ethical Committee for the study were not necessary because the blood for all analyses was taken during a routine veterinary examination of animals admitted to the University\u2019s Small Animal Veterinary Practice for preventive screening, and additional cardiac examination was offered to these individuals at no further cost. However, before enrolling a dog into the study, written informed consent for a full cardiac examination and blood sampling was obtained from the owner and a high standard of care was adhered to throughout each examination. The study was carried out in compliance with the ARRIVE guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"210"}}