{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T18:25:39Z","timestamp":1776709539930,"version":"3.51.2"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T00:00:00Z","timestamp":1720137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T00:00:00Z","timestamp":1720137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002386","name":"Cairo University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002386","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Senescence is accompanied by a progressive decrease in male reproductive performance, mainly due to oxidative stress and endothelial dysfunction. Alpha lipoic acid (ALA) is a potent antioxidant, that diffuses freely in aqueous and lipid phases, possessing anti-inflammatory and anti-apoptotic properties. This study aimed to examine the effects of supplemental dietary ALA on testicular hemodynamics (TH), circulating hormones, and semen quality in aged goats. Twelve Baladi bucks were divided into two groups (<jats:italic>n<\/jats:italic>\u2009=\u20096 each); the first fed a basic ration and served as a control group (CON), while the second received the basic ration supplemented with 600\u00a0mg ALA\/ kg daily for consecutive eight weeks (ALA).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>There were improvements in testicular blood flow in the ALA group evidenced by a lower resistance index (RI) and pulsatility index (PI) concurrent with higher pampiniform-colored areas\/pixel (W3-W6). There were increases in testicular volume and decreases in echogenicity (W3-W5; ALA vs. CON). Compared to the CON, ALA-bucks had higher serum concentrations of testosterone, estradiol, and nitric oxide (W3-W5). There were enhancements in semen traits (progressive motility, viability, morphology, and concentration, alanine aminotransferase enzyme) and oxidative biomarkers (catalase, total antioxidant capacity, and malondialdehyde).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>ALA dietary supplementation (600\u00a0mg\/kg diet) improved aged bucks\u2019 reproductive performance by enhancing the testicular volume, testicular hemodynamics, sex steroids, and semen quality.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-04134-4","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T05:01:47Z","timestamp":1720155707000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Outcomes of dietary alpha-lipoic acid on testicular vascularization, steroid hormones, and seminal quality in aged Baladi bucks"],"prefix":"10.1186","volume":"20","author":[{"given":"Elshymaa A.","family":"Abdelnaby","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Fathi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noha Y.","family":"Salem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman S.","family":"Ramadan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimaa G.","family":"Yehia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibrahim A.","family":"Emam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Salama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haney","family":"Samir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossam R.","family":"El-Sherbiny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"4134_CR1","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.1038\/nm.4000","volume":"21","author":"BG Childs","year":"2015","unstructured":"Childs BG, Durik M, Baker DJ, Van Deursen JM. Cellular senescence in aging and age-related disease: from mechanisms to therapy. Nat Med. 2015;21:1424\u201335.","journal-title":"Nat Med"},{"key":"4134_CR2","doi-asserted-by":"publisher","first-page":"e14309","DOI":"10.1111\/and.14309","volume":"54","author":"M Hedia","year":"2022","unstructured":"Hedia M, El-Shalofy A. Ageing affects plasma steroid concentrations and testicular volume, echotexture and haemodynamics in rams. Andrologia. 2022;54:e14309.","journal-title":"Andrologia"},{"key":"4134_CR3","doi-asserted-by":"publisher","first-page":"757","DOI":"10.2147\/CIA.S158513","volume":"13","author":"I Liguori","year":"2018","unstructured":"Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stressin, aging, and diseases. Clin Interv Aging. 2018;13:757.","journal-title":"Clin Interv Aging"},{"key":"4134_CR4","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1016\/j.psj.2020.10.076","volume":"100","author":"A Behnamifar","year":"2021","unstructured":"Behnamifar A, Rahimi S, Torshizi MAK, Sharafi M, Grimes J. Effects of dietary alpha-lipoic acid supplementation on the seminal parameters and fertility potential in aging broiler breeder roosters. Poult Sci. 2021;100:1221\u201338.","journal-title":"Poult Sci"},{"key":"4134_CR5","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.3390\/antiox10081283","volume":"10","author":"MN De Luca","year":"2021","unstructured":"De Luca MN, Colone M, Gambioli R, Stringaro A, Unfer V. Oxidative stress and male fertility: role of antioxidants and inositols. Antioxidants. 2021;10:1283.","journal-title":"Antioxidants"},{"key":"4134_CR6","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.3390\/antiox10071154","volume":"10","author":"E Pintus","year":"2021","unstructured":"Pintus E, Ros-Santaella JL. Impact of oxidative stress on male reproduction in domestic and wild animals. Antioxidants. 2021;10:1154.","journal-title":"Antioxidants"},{"key":"4134_CR7","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1080\/10495398.2019.1657126","volume":"32","author":"RR da Rosa Filho","year":"2021","unstructured":"da Rosa Filho RR, Angrimani DS, Brito MM, Nichi M, Vannucchi CI, Lucio CF. Susceptibility of epididymal sperm against reactive oxygen species in dogs. Animal Biotechnol. 2021;32:92\u20139.","journal-title":"Animal Biotechnol"},{"key":"4134_CR8","doi-asserted-by":"publisher","first-page":"1700","DOI":"10.1016\/j.theriogenology.2012.05.002","volume":"78","author":"H Guthrie","year":"2012","unstructured":"Guthrie H, Welch G. Effects of reactive oxygen species on sperm function. Theriogenology. 2012;78:1700\u20138.","journal-title":"Theriogenology"},{"key":"4134_CR9","doi-asserted-by":"publisher","first-page":"21","DOI":"10.3164\/jcbn.14-130","volume":"57","author":"S Silvestri","year":"2015","unstructured":"Silvestri S, Orlando P, Armeni T, Padella L, Brug\u00e8 F, Seddaiu G, et al. Coenzyme Q10 and \u03b1-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects. J Clin Biochem Nutr. 2015;57:21\u20136.","journal-title":"J Clin Biochem Nutr"},{"key":"4134_CR10","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1016\/S0531-5565(01)00118-8","volume":"36","author":"H Chen","year":"2001","unstructured":"Chen H, Cangello D, Benson S, Folmer J, Zhu H, Trush MA, et al. Age-related increase in mitochondrial superoxide generation in the testosterone-producing cells of Brown Norway rat testes: relationship to reduced steroidogenic function? Exp Gerontol. 2001;36:1361\u201373.","journal-title":"Exp Gerontol"},{"key":"4134_CR11","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/S1734-1140(11)70600-4","volume":"63","author":"A Gor\u0105ca","year":"2011","unstructured":"Gor\u0105ca A, Huk-Kolega H, Piechota A, Kleniewska P, Ciejka E, Skibska B. Lipoic acid\u2013biological activity and therapeutic potential. Pharmacol Rep. 2011;63:849\u201358.","journal-title":"Pharmacol Rep"},{"key":"4134_CR12","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1096\/fj.00-0176com","volume":"15","author":"JH SUH","year":"2001","unstructured":"SUH JH, Shigeno ET, Morrow JD, COX JB, ROCHA AE. Oxidative stress in the aging rat heart is reversed by dietary supplementation with (R)-\u03b1\u2010lipoic acid. FASEB J. 2001;15:700\u20136.","journal-title":"FASEB J"},{"key":"4134_CR13","first-page":"167","volume":"3","author":"SC Zicker","year":"2002","unstructured":"Zicker SC, Hagen TM, Joisher N, Golder C, Joshi DK, Miller EP. Safety of long-term feeding of dl-alpha-lipoic acid and its effect on reduced glutathione: oxidized glutathione ratios in beagles. Veterinary Therapeutics: Res Appl Veterinary Med. 2002;3:167\u201376.","journal-title":"Veterinary Therapeutics: Res Appl Veterinary Med"},{"key":"4134_CR14","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/j.exger.2004.01.007","volume":"39","author":"NW Milgram","year":"2004","unstructured":"Milgram NW, Head E, Zicker SC, Ikeda-Douglas C, Murphey H, Muggenberg BA, et al. Long-term treatment with antioxidants and a program of behavioral enrichment reduces age-dependent impairment in discrimination and reversal learning in beagle dogs. Exp Gerontol. 2004;39:753\u201365.","journal-title":"Exp Gerontol"},{"key":"4134_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s11250-021-02917-7","volume":"53","author":"H Zhang","year":"2021","unstructured":"Zhang H, Yang G, Li H, Wang L, Fu T, Li G, et al. Effects of dietary supplementation with alpha-lipoic acid on apparent digestibility and serum metabolome alterations of sheep in summer. Trop Anim Health Prod. 2021;53:1\u201313.","journal-title":"Trop Anim Health Prod"},{"key":"4134_CR16","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1080\/1828051X.2016.1263546","volume":"16","author":"D Wang","year":"2017","unstructured":"Wang D, Zhou L, Zhou H, Hou G, Shi L. Effects of dietary \u03b1-lipoic acid on carcass characteristics, antioxidant capability and meat quality in Hainan black goats. Italian J Anim Sci. 2017;16:61\u20137.","journal-title":"Italian J Anim Sci"},{"key":"4134_CR17","doi-asserted-by":"crossref","unstructured":"El-Sherbiny H, Abdelnaby E, El-Shahat K, Salem N, Ramadan E, Yehia S et al. Coenzyme Q10 Supplementation enhances testicular volume and hemodynamics, reproductive hormones, sperm quality, and seminal antioxidant capacity in goat bucks under summer hot humid conditions. Vet Res Commun 2022.","DOI":"10.1007\/s11259-022-09991-8"},{"key":"4134_CR18","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.theriogenology.2012.01.022","volume":"78","author":"L Brito","year":"2012","unstructured":"Brito L, Barth A, Wilde R, Kastelic J. Testicular ultrasonogram pixel intensity during sexual development and its relationship with semen quality, sperm production, and quantitative testicular histology in beef bulls. Theriogenology. 2012;78:69\u201376.","journal-title":"Theriogenology"},{"key":"4134_CR19","doi-asserted-by":"publisher","first-page":"85","DOI":"10.5958\/0973-9718.2022.00034.4","volume":"28","author":"H El-Sherbiny","year":"2022","unstructured":"El-Sherbiny H, Shahat A, Hedia M, El-Shalofy A. Effect of sexual maturation on testicular morphometry and echotexture and their association with intratesticular blood flow in ossimi rams. Indian J Small Ruminants (the). 2022;28:85\u201390.","journal-title":"Indian J Small Ruminants (the)"},{"key":"4134_CR20","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1111\/rda.14117","volume":"57","author":"EA Abdelnaby","year":"2022","unstructured":"Abdelnaby EA. Testicular haemodynamics, plasma testosterone and oestradiol concentrations, and serum nitric oxide levels in the Egyptian buffalo bull after a single administration of human chorionic gonadotropin. Reprod Domest Anim. 2022;57:754\u201360.","journal-title":"Reprod Domest Anim"},{"key":"4134_CR21","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1111\/rda.14096","volume":"57","author":"AM Fadl","year":"2022","unstructured":"Fadl AM, Abdelnaby EA, El-Sherbiny HR. Supplemental dietary zinc sulphate and folic acid combination improves testicular volume and haemodynamics, testosterone levels and semen quality in rams under heat stress conditions. Reprod Domest Anim. 2022;57:567\u201376.","journal-title":"Reprod Domest Anim"},{"key":"4134_CR22","doi-asserted-by":"publisher","first-page":"860771","DOI":"10.3389\/fvets.2022.860771","volume":"9","author":"HR El-Sherbiny","year":"2022","unstructured":"El-Sherbiny HR, El-Shalofy AS, Samir H. Exogenous L-carnitine administration ameliorates the adverse effects of heat stress on testicular hemodynamics, echotexture, and total antioxidant capacity in rams. Front Veterinary Sci. 2022;9:860771.","journal-title":"Front Veterinary Sci"},{"key":"4134_CR23","doi-asserted-by":"publisher","first-page":"1442","DOI":"10.3390\/ani12111442","volume":"12","author":"NM Hashem","year":"2022","unstructured":"Hashem NM, El-Sherbiny HR, Fathi M, Abdelnaby EA. Nanodelivery System for Ovsynch Protocol improves ovarian response, ovarian blood Flow Doppler velocities, and Hormonal Profile of goats. Animals. 2022;12:1442.","journal-title":"Animals"},{"key":"4134_CR24","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1111\/rda.13696","volume":"55","author":"MG Hedia","year":"2020","unstructured":"Hedia MG, El-Belely MS, Ismail ST, Abo El\u2010Maaty AM. Seasonal variation in testicular blood flow dynamics and their relation to systemic and testicular oxidant\/antioxidant biomarkers and androgens in rams. Reprod Domest Anim. 2020;55:861\u20139.","journal-title":"Reprod Domest Anim"},{"key":"4134_CR25","doi-asserted-by":"publisher","first-page":"2188","DOI":"10.1016\/j.theriogenology.2006.05.020","volume":"66","author":"C Hansen","year":"2006","unstructured":"Hansen C, Vermeiden T, Vermeiden J, Simmet C, Day B, Feitsma H. Comparison of FACSCount AF system, Improved Neubauer hemocytometer, Corning 254 photometer, SpermVision, UltiMate and NucleoCounter SP-100 for determination of sperm concentration of boar semen. Theriogenology. 2006;66:2188\u201394.","journal-title":"Theriogenology"},{"key":"4134_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12917-022-03390-6","volume":"18","author":"EA Abdelnaby","year":"2022","unstructured":"Abdelnaby EA, Yasin NA, Abouelela YS, Rashad E, Daghash SM, El-Sherbiny HR. Ovarian, uterine, and luteal vascular perfusions during follicular and luteal phases in the adult cyclic female rabbits with special orientation to their histological detection of hormone receptor. BMC Vet Res. 2022;18:1\u201318.","journal-title":"BMC Vet Res"},{"key":"4134_CR27","doi-asserted-by":"publisher","first-page":"262","DOI":"10.15547\/bjvm.2020-0091","volume":"25","author":"H El-Sherbiny","year":"2022","unstructured":"El-Sherbiny H, El-Shahat K, Abo EL, Abdelnaby E. Ovarian and uterine haemodynamics and their relation to steroid hormonal levels in postpartum Egyptian buffaloes. Bulgarian J Veterinary Med. 2022;25:262\u201373.","journal-title":"Bulgarian J Veterinary Med"},{"key":"4134_CR28","doi-asserted-by":"crossref","unstructured":"Samir H, El-Shalofy A, El-Sherbiny HR. Effects of a single dose of long-acting FSH on testicular blood flow, testicular echotexture, and circulating testosterone, estradiol, and nitric oxide in rams during the non-breeding season. Domest Anim Endocrinol 2023:106765.","DOI":"10.1016\/j.domaniend.2022.106765"},{"key":"4134_CR29","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/S0891-5849(01)00551-2","volume":"31","author":"T Heitzer","year":"2001","unstructured":"Heitzer T, Finckh B, Albers S, Krohn K, Kohlsch\u00fctter A, Meinertz T. Beneficial effects of \u03b1-lipoic acid and ascorbic acid on endothelium-dependent, nitric oxide-mediated vasodilation in diabetic patients: relation to parameters of oxidative stress. Free Radic Biol Med. 2001;31:53\u201361.","journal-title":"Free Radic Biol Med"},{"key":"4134_CR30","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1248\/yakushi.131.739","volume":"131","author":"N Okudan","year":"2011","unstructured":"Okudan N, Atalik KN, G\u00f6kbel H, Canbilen A, Kara I. Alpha lipoic acid treatment improved endothelium-dependent relaxation in diabetic rat aorta. Yakugaku Zasshi. 2011;131:739\u201344.","journal-title":"Yakugaku Zasshi"},{"key":"4134_CR31","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.diabres.2014.11.005","volume":"107","author":"M Dworacka","year":"2015","unstructured":"Dworacka M, Iskakova S, Krzy\u017cag\u00f3rska E, Weso\u0142owska A, Kurmambayev Y, Dworacki G. Alpha-lipoic acid modifies circulating angiogenic factors in patients with type 2 diabetes mellitus. Diabetes Res Clin Pract. 2015;107:273\u20139.","journal-title":"Diabetes Res Clin Pract"},{"key":"4134_CR32","first-page":"23","volume":"50","author":"SB Budin","year":"2009","unstructured":"Budin SB, Othman F, Louis S, Bakar MA, Radzi M, Osman K, et al. Effect of alpha lipoic acid on oxidative stress and vascular wall of diabetic rats. Rom J Morphol Embryol. 2009;50:23\u201330.","journal-title":"Rom J Morphol Embryol"},{"key":"4134_CR33","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.theriogenology.2022.05.020","volume":"188","author":"HR El-Sherbiny","year":"2022","unstructured":"El-Sherbiny HR, Fathi M, Samir H, Abdelnaby EA. Supplemental dietary curcumin improves testicular hemodynamics, testosterone levels, and semen quality in Baladi bucks in the non-breeding season. Theriogenology. 2022;188:100\u20137.","journal-title":"Theriogenology"},{"key":"4134_CR34","doi-asserted-by":"publisher","first-page":"e13085","DOI":"10.1111\/and.13085","volume":"50","author":"E Shaygannia","year":"2018","unstructured":"Shaygannia E, Tavalaee M, Akhavanfarid GR, Rahimi M, Dattilo M, Nasr-Esfahani MH. Alpha\u2010lipoic acid improves the testicular dysfunction in rats induced by varicocele. Andrologia. 2018;50:e13085.","journal-title":"Andrologia"},{"key":"4134_CR35","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.theriogenology.2020.10.017","volume":"159","author":"N Ye","year":"2021","unstructured":"Ye N, Lv Z, Dai H, Huang Z, Shi F. Dietary alpha-lipoic acid supplementation improves spermatogenesis and semen quality via antioxidant and anti-apoptotic effects in aged breeder roosters. Theriogenology. 2021;159:20\u20137.","journal-title":"Theriogenology"},{"key":"4134_CR36","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1007\/s00011-017-1079-6","volume":"66","author":"D Tibullo","year":"2017","unstructured":"Tibullo D, Li Volti G, Giallongo C, Grasso S, Tomassoni D, Anfuso CD, et al. Biochemical and clinical relevance of alpha lipoic acid: antioxidant and anti-inflammatory activity, molecular pathways and therapeutic potential. Inflamm Res. 2017;66:947\u201359.","journal-title":"Inflamm Res"},{"key":"4134_CR37","first-page":"e13990","volume":"53","author":"EE Guzel","year":"2021","unstructured":"Guzel EE, Tektemur NK, Tektemur A. Alpha-lipoic acid may ameliorate testicular damage by targeting dox-induced altered antioxidant parameters, mitofusin-2 and apoptotic gene expression. Andrologia. 2021;53:e13990.","journal-title":"Andrologia"},{"key":"4134_CR38","doi-asserted-by":"publisher","first-page":"107741","DOI":"10.1016\/j.intimp.2021.107741","volume":"96","author":"S Sheikholeslami","year":"2021","unstructured":"Sheikholeslami S, Khodaverdian S, Dorri-Giv M, Hosseini SM, Souri S, Abedi-Firouzjah R, et al. The radioprotective effects of alpha-lipoic acid on radiotherapy-induced toxicities: a systematic review. Int Immunopharmacol. 2021;96:107741.","journal-title":"Int Immunopharmacol"},{"key":"4134_CR39","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.theriogenology.2022.09.029","volume":"194","author":"H El-Sherbiny","year":"2022","unstructured":"El-Sherbiny H, Abdelnaby E, Samir H, Fathi M. Addition of autologous platelet rich plasma to semen extender enhances cryotolerance and fertilizing capacity of buffalo bull spermatozoa. Theriogenology. 2022;194:104\u20139.","journal-title":"Theriogenology"},{"key":"4134_CR40","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1590\/S1807-59322008000400022","volume":"63","author":"SF Ibrahim","year":"2008","unstructured":"Ibrahim SF, Osman K, Das S, Othman AM, Majid NA, Rahman MPA. A study of the antioxidant effect of alpha lipoic acids on sperm quality. Clinics. 2008;63:545\u201350.","journal-title":"Clinics"},{"key":"4134_CR41","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.fertnstert.2015.05.014","volume":"104","author":"HK Haghighian","year":"2015","unstructured":"Haghighian HK, Haidari F, Mohammadi-Asl J, Dadfar M. Randomized, triple-blind, placebo-controlled clinical trial examining the effects of alpha-lipoic acid supplement on the spermatogram and seminal oxidative stress in infertile men. Fertil Steril. 2015;104:318\u201324.","journal-title":"Fertil Steril"},{"key":"4134_CR42","doi-asserted-by":"publisher","first-page":"100","DOI":"10.4103\/2305-0500.341117","volume":"11","author":"AM Fadl","year":"2022","unstructured":"Fadl AM, Abdelnaby EA, El-Sherbiny HR. INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages. Asian Pac J Reprod. 2022;11:100\u20134.","journal-title":"Asian Pac J Reprod"},{"key":"4134_CR43","first-page":"259","volume":"12","author":"A Fadl","year":"2022","unstructured":"Fadl A, Abdelnaby E, El-seadawy I, Kotp M, El-Maaty AMA, El-Sherbiny H. Eco-friendly synthesized zinc Oxide nanoparticles Improved Frozen-thawed semen quality and antioxidant capacity of rams. J Adv Veterinary Res. 2022;12:259\u201364.","journal-title":"J Adv Veterinary Res"},{"key":"4134_CR44","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1002\/jnr.23289","volume":"92","author":"S Grasso","year":"2014","unstructured":"Grasso S, Bramanti V, Tomassoni D, Bronzi D, Malfa G, Traini E, et al. Effect of lipoic acid and \u03b1-glyceryl\u2010phosphoryl\u2010choline on astroglial cell proliferation and differentiation in primary culture. J Neurosci Res. 2014;92:86\u201394.","journal-title":"J Neurosci Res"},{"issue":"2\u20133","key":"4134_CR45","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.smallrumres.2011.04.006","volume":"99","author":"H Ma","year":"2011","unstructured":"Ma H, Quan F, Chen D, Zheng Y, Zhang B, Wang Y, Zhang Y. Protective function of alpha-lipoic acid on sperm motility and mitochondrial function during goat sperm-mediated gene transfer. Small Ruminant Res. 2011;99(2\u20133):191\u20138.","journal-title":"Small Ruminant Res"},{"key":"4134_CR46","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.cryobiol.2018.08.006","volume":"84","author":"F Ren","year":"2018","unstructured":"Ren F, Feng T, Dai G, Wang Y, Zhu H, Hu J. Lycopene and alpha-lipoic acid improve semen antioxidant enzymes activity and cashmere goat sperm function after cryopreservation. Cryobiology. 2018;84:27\u201332.","journal-title":"Cryobiology"},{"key":"4134_CR47","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/j.psj.2020.10.007","volume":"100","author":"A Najafi","year":"2021","unstructured":"Najafi A, Kia HD, Hamishehkar H. Does alpha-lipoic acid\u2013loaded nanostructured lipid carriers improve post-thawed sperm quality and ameliorate apoptosis-related genes of rooster sperm? Poult Sci. 2021;100:357\u201365.","journal-title":"Poult Sci"},{"key":"4134_CR48","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1002\/j.1939-4640.2000.tb03420.x","volume":"21","author":"J Baumber","year":"2000","unstructured":"Baumber J, Ball BA, Gravance CG, Medina V, Davies-Morel MC. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J Androl. 2000;21:895\u2013902.","journal-title":"J Androl"},{"key":"4134_CR49","first-page":"835","volume":"67","author":"A Bidmeshkipour","year":"2010","unstructured":"Bidmeshkipour A, Hosseinzadeh Colagar A, Gholinezhad Chari M, Biparva P. Seminal plasma total antioxidant capacity and vitamin-C levels in asthenozoospermia: a case-control study. Tehran Univ Med J. 2010;67:835\u201342.","journal-title":"Tehran Univ Med J"},{"key":"4134_CR50","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1017\/S135772980005517X","volume":"71","author":"T Taha","year":"2000","unstructured":"Taha T, Abdel-Gawad E, Ayoub M. Monthly variations in some reproductive parameters of Barki and Awassi rams throughout 1 year under subtropical conditions 2. Biochemical and enzymatic properties of seminal plasma. Anim Sci. 2000;71:325\u201332.","journal-title":"Anim Sci"},{"issue":"1","key":"4134_CR51","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1186\/s12917-023-03692-3","volume":"19","author":"EA Abdelnaby","year":"2023","unstructured":"Abdelnaby EA, Alhaider AK, El-Maaty AMA, Ragab RSA, Seida AA, El-Badry DA. Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation. BMC Vet Res. 2023;19(1):205. https:\/\/doi.org\/10.1186\/s12917-023-03692-3. PMID: 37833782; PMCID: PMC10571355.","journal-title":"BMC Vet Res"},{"issue":"1","key":"4134_CR52","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1186\/s12917-023-03745-7","volume":"19","author":"H Madbouly","year":"2023","unstructured":"Madbouly H, El-Shahat KH, Fathi M, Abdelnaby EA. Hemodynamic changes in late advanced pregnant Zaraibi goats during the peripartum period. BMC Vet Res. 2023;19(1):194. https:\/\/doi.org\/10.1186\/s12917-023-03745-7. PMID: 37803319; PMCID: PMC10559465.","journal-title":"BMC Vet Res"},{"issue":"1","key":"4134_CR53","doi-asserted-by":"publisher","first-page":"100855","DOI":"10.1016\/j.repbio.2024.100855","volume":"24","author":"EA Abdelnaby","year":"2024","unstructured":"Abdelnaby EA, Alhaider AK, Ghoneim IM, Salem NY, Ramadan ES, Farghali HA, Khattab MS, AbdElKader NA, Emam IA. Effect of pyometra on vascularity alterations, oxidative stress, histopathology and inflammatory molecules in feline. Reprod Biol. 2024;24(1):100855. Epub 2024 Jan 22. PMID: 38262266.","journal-title":"Reprod Biol"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-024-04134-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-024-04134-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-024-04134-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T06:05:41Z","timestamp":1720159541000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-024-04134-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,5]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["4134"],"URL":"https:\/\/doi.org\/10.1186\/s12917-024-04134-4","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,5]]},"assertion":[{"value":"19 January 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animals were treated after the acceptance of the ethical approval from the Veterinary Medicine Cairo University Institutional Animal Care (Vet CU 2009-2022-492). All methods were performed in accordance with relevant guidelines and regulations. All methods are reported in accordance with ARRIVE guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not Applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All authors declare any conflict of interest.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"293"}}