{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:36:52Z","timestamp":1789000612346,"version":"build-2803163510"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007726","name":"Shahid Bahonar University of Kerman","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Rickettsia<\/jats:italic> occurs worldwide and rickettsiosis is recognized as an emerging infection in several parts of the world. Ticks are reservoir hosts for pathogenic <jats:italic>Rickettsia<\/jats:italic> species in humans and domestic animals. Most pathogenic <jats:italic>Rickettsia<\/jats:italic> species belong to the spotted Fever Group (SFG). This study aimed to identify and diagnose tick fauna and investigate the prevalence of <jats:italic>Rickettsia<\/jats:italic> spp. in ticks collected from domestic animals and dogs in the rural regions of Kerman Province, Southeast Iran. In this study, tick species (fauna) were identified and 2100 ticks (350 pooled samples) from two genera and species including <jats:italic>Rhipicephalus linnaei<\/jats:italic> (1128) and <jats:italic>Hyalomma deteritum<\/jats:italic> (972) were tested to detect <jats:italic>Rickettsia<\/jats:italic> genus using Real-time PCR. The presence of the <jats:italic>Rickettsia<\/jats:italic> genus was observed in 24.9% (95%CI 20.28\u201329.52) of the pooled samples. Sequencing and phylogenetic analyses revealed the presence of <jats:italic>Rickettsia aeschlimannii<\/jats:italic> (48.98%), <jats:italic>Rickettsia conorii israelensis<\/jats:italic> (28.57%), <jats:italic>Rickettsia sibirica<\/jats:italic> (20.41%), and <jats:italic>Rickettsia helvetica<\/jats:italic> (2.04%) in the positive samples. The results showed a significant association between county variables and the following variables: tick spp. (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), <jats:italic>Rickettsia<\/jats:italic> genus infection in ticks (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and <jats:italic>Rickettsia<\/jats:italic> spp. (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In addition, there was a significant association between tick species and host animals (dogs and domestic animals) (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), <jats:italic>Rickettsia<\/jats:italic> spp infection in ticks (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and <jats:italic>Rickettsia<\/jats:italic> spp. (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). This study indicates a high prevalence of <jats:italic>Rickettsia<\/jats:italic> spp. (SFG) in ticks of domestic animals and dogs in rural areas of Kerman Province. The health system should be informed of the possibility of rickettsiosis and the circulating species of <jats:italic>Rickettsia<\/jats:italic> in these areas.<\/jats:p>","DOI":"10.1186\/s12917-024-04142-4","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T05:01:52Z","timestamp":1719464512000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Diversity of Rickettsia species in collected ticks from Southeast Iran"],"prefix":"10.1186","volume":"20","author":[{"given":"Ali","family":"Qorbani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Khalili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeidreza","family":"Nourollahifard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Mostafavi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrdad","family":"Farrokhnia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saber","family":"Esmaeili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"issue":"19","key":"4142_CR1","doi-asserted-by":"publisher","first-page":"4431","DOI":"10.3390\/molecules25194431","volume":"25","author":"V Blanda","year":"2020","unstructured":"Blanda V, et al. New real-time PCRs to differentiate Rickettsia spp. and Rickettsia conorii. Molecules. 2020;25(19):4431.","journal-title":"Molecules"},{"key":"4142_CR2","doi-asserted-by":"crossref","unstructured":"Mansueto P et al. New insight into immunity and immunopathology of Rickettsial diseases Clinical and Developmental Immunology, 2012. 2012.","DOI":"10.1155\/2012\/967852"},{"issue":"1","key":"4142_CR3","doi-asserted-by":"publisher","first-page":"83","DOI":"10.3390\/insects12010083","volume":"12","author":"N Perveen","year":"2021","unstructured":"Perveen N, Muzaffar SB, Al-Deeb MA. Ticks and tick-borne diseases of livestock in the Middle East and North Africa: a review. Insects. 2021;12(1):83.","journal-title":"Insects"},{"issue":"8","key":"4142_CR4","doi-asserted-by":"publisher","first-page":"e01728","DOI":"10.1128\/JCM.01728-17","volume":"56","author":"MY Abdad","year":"2018","unstructured":"Abdad MY, et al. A concise review of the epidemiology and diagnostics of rickettsioses: Rickettsia and Orientia spp. J Clin Microbiol. 2018;56(8):e01728\u201317.","journal-title":"J Clin Microbiol"},{"key":"4142_CR5","doi-asserted-by":"crossref","unstructured":"Afzalkhani A et al. Molecular detection and diversity of spotted fever group Rickettsia isolated from ticks in Iran 2022.","DOI":"10.21203\/rs.3.rs-1324430\/v1"},{"issue":"3","key":"4142_CR6","first-page":"256","volume":"12","author":"M Farrokhnia","year":"2020","unstructured":"Farrokhnia M, et al. Cases of Mediterranean spotted fever in southeast of Iran. Iran J Microbiol. 2020;12(3):256.","journal-title":"Iran J Microbiol"},{"key":"4142_CR7","unstructured":"Walker AR. Ticks of domestic animals in Africa: a guide to identification of species. Bioscience Reports Edinburgh; 2003."},{"key":"4142_CR8","doi-asserted-by":"crossref","unstructured":"\u0160lapeta J, et al. 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UK. VETMED. REC. 1399, 025) and all methods were performed under relevant guidance and regulations. The oral informed consent from the domestic animal owners was approved by the Ethics Committee of Shahid Bahonar University of Kerman. All methods were carried out according to the relevant guidelines and regulations. This study was approved by. The verbal permission of Informed consent was taken from the domestic animal owners for the collection of ticks.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"279"}}